A Recombinant Avian Infectious Bronchitis Virus Expressing a Heterologous Spike Gene Belonging to the 4/91 Serotype

We have shown previously that replacement of the spike (S) gene of the apathogenic IBV strain Beau-R with that from the pathogenic strain of the same serotype, M41, resulted in an apathogenic virus, BeauR-M41(S), that conferred protection against challenge with M41 [1]. We have constructed a recombinant IBV, BeauR-4/91(S), with the genetic backbone of Beau-R but expressing the spike protein of the pathogenic IBV strain 4/91(UK), which belongs to a different serogroup as Beaudette or M41. Similar to our previous findings with BeauR-M41(S), clinical signs observations showed that the S gene of the pathogenic 4/91 virus did not confer pathogenicity to the rIBV BeauR-4/91(S). Furthermore, protection studies showed there was homologous protection; BeauR-4/91(S) conferred protection against challenge with wild type 4/91 virus as shown by the absence of clinical signs, IBV RNA assessed by qRT-PCR and the fact that no virus was isolated from tracheas removed from birds primarily infected with BeauR-4/91(S) and challenged with IBV 4/91(UK). A degree of heterologous protection against M41 challenge was observed, albeit at a lower level

Magnetic field generation in plasma waves driven by copropagating intense twisted lasers

We present a new magnetic field generation mechanism in underdense plasmas driven by the beating of two, copropagating, Laguerre-Gaussian orbital angular momentum laser pulses with different frequencies and also different twist indices. The resulting twisted ponderomotive force drives up an electron plasma wave with a helical rotating structure. To second order, there is a nonlinear rotating current leading to the onset of an intense, static axial magnetic field, which persists over a long time in the plasma (ps scale) after the laser pulses have passed by. The results are confirmed in three-dimensional particle-in-cell simulations and also theoretical analysis. For the case of 300 fs duration, 3.8×1017  W/cm2 peak laser intensity we observe magnetic field of up to 0.4 MG. This new method of magnetic field creation may find applications in charged beam collimation and microscale pinch