Rola komórek Langerhansa w immunopatogenezie atopowego zapalenia skóry

Wprowadzenie: Atopowe zapalenie skóry (AZS) jest często występującą,przewlekłą chorobą zapalną, w której stwierdza się liczne nieprawidłowościimmunologiczne, m.in.: wysokie stężenie IgE, nadmierną aktywnośćkomórek Langerhansa (KL) oraz zwiększoną ekspresję receptoraCD23. Komórki Langerhansa są komórkami dendrytycznymi pochodzeniaszpikowego. Stanowią one 3–8% wszystkich komórek naskórka,a u osób z chorobami alergicznymi jest ich znacznie więcej. Komórki Langerhansasą zdolne wychwytywać antygeny egzogenne, takie jak haptenyczy antygeny wirusowe, przetwarzać je i prezentować limfocytom T.Komórki Langerhansa na swojej powierzchni mają antygeny HLA-DR,antygen CD4, receptory FcεRIα i CD23, receptory dla przeciwciała IgEi CD1a, a także cząs teczki kostymulujące CD 80/B7-1, CD 86/B7-2. Cel pracy: Ustalenie liczby i cech morfologicznych komórek Langerhansaw naskórku chorych na AZS przy użyciu przeciwciał przeciwantygenom CD1a i HLA-DR w porównaniu z osobami zdrowymi orazoznaczenie receptorów powierzchniowych FcεRIα, CD4, CD23,CD80/B7-1, CD86/B7-2, IgE. Materiał i metodyka: Przebadano 18 wycinków pochodzących od osóbchorych na atopowe zapalenie skóry. Próbę kontrolną stanowiły wycinkiod 10 zdrowych osób pobrane z pośladków lub z grzbietu ręki. Biopsjęu osób chorych pobierano ze zmian chorobowych. Markery KL (CD1a,HLA-DR, FcεRIα, CD4, CD23, CD80/B7-1, CD86/B7-2, IgE) wykrywano,stosując podwójne barwienie immunofluorescencyjne. Wyniki: U pacjentów z AZS stwierdzono w naskórku znacznie więcejKL CD1a+ niż u zdrowych. Wygląd KL w naskórku różnił się u osóbchorych i zdrowych. W naskórku chorych na powierzchni KL stwierdzonoobecność wszystkich badanych receptorów, a u zdrowych tylkoCD1a, HLA-DR, FcεRIα. Wnioski: Rola KL zależy od obecności receptorów na ich powierzchni,a ich morfologia w naskórku osób chorych na AZS i w naskórku osóbzdrowych znacznie się różni. Bardzo duża liczba KL w naskórkuchorych na AZS świadczy o stanie zapalnym

Vitamin D status in patients with rheumatoid arthritis: a correlation analysis with disease activity and progression, as well as serum IL-6 levels

Objectives. Recent epidemiological studies suggested an association between a poor vitamin D [25(OH)D] status, inflammatory mediators, and rheumatoid arthritis (RA). We have recently proposed that pro-inflammatory interleukin 6 (IL-6) may represent a good marker for disease activity of RA. The aim of this study was to investigate the relationship between serum 25(OH)D levels and disease activity, joint damage, as well as serum IL-6 levels in a Polish RA population. Materials and Methods. Serum 25(OH)D levels were measured in 35 female RA patients and 38 age- and gender-matched healthy controls. Statistical correlations between 25(OH)D levels and the disease activity score 28 (DAS 28), joint damage based on the Steinbrocker criteria, as well as serum IL-6 levels were performed. Results. There was no statistically significant difference between levels of 25(OH)D in RA (16.89±8.57 ng/ml) and healthy controls (14.12±7.51 ng/ml), and the vitamin D deficiency (<20 ng/ml) was found in 71.43% of RA patients and 73.68 % of healthy controls. While vitamin D status did not correlate with DAS 28 (r=0.265, p=0.149) and joint damage based on the Steinbrocker criteria (r=0.367, p=0.065), a positive correlation between 25(OH)D and IL-6 (r=0.537, p=0.002) was observed in RA. Conclusion. Although further studies on a larger group of patients will be needed to confirm the data presented here, it seems that hypovitaminosis D is common in the RA patients and middle-aged non-RA healthy women in the Polish population. 25(OH)D levels were similar in the RA patients and age- and gender-matched healthy controls, and were not associated with joint damage and disease activity in patients

Vitamin D status in patients with rheumatoid arthritis: a correlation analysis with disease activity and progression, as well as serum IL-6 levels

Objectives. Recent epidemiological studies suggested an association between a poor vitamin D [25(OH)D] status, inflammatory mediators, and rheumatoid arthritis (RA). We have recently proposed that pro-inflammatory interleukin 6 (IL-6) may represent a good marker for disease activity of RA. The aim of this study was to investigate the relationship between serum 25(OH)D levels and disease activity, joint damage, as well as serum IL-6 levels in a Polish RA population. Materials and Methods. Serum 25(OH)D levels were measured in 35 female RA patients and 38 age- and gender-matched healthy controls. Statistical correlations between 25(OH)D levels and the disease activity score 28 (DAS 28), joint damage based on the Steinbrocker criteria, as well as serum IL-6 levels were performed. Results. There was no statistically significant difference between levels of 25(OH)D in RA (16.89±8.57 ng/ml) and healthy controls (14.12±7.51 ng/ml), and the vitamin D deficiency (<20 ng/ml) was found in 71.43% of RA patients and 73.68 % of healthy controls. While vitamin D status did not correlate with DAS 28 (r=0.265, p=0.149) and joint damage based on the Steinbrocker criteria (r=0.367, p=0.065), a positive correlation between 25(OH)D and IL-6 (r=0.537, p=0.002) was observed in RA. Conclusion. Although further studies on a larger group of patients will be needed to confirm the data presented here, it seems that hypovitaminosis D is common in the RA patients and middle-aged non-RA healthy women in the Polish population. 25(OH)D levels were similar in the RA patients and age- and gender-matched healthy controls, and were not associated with joint damage and disease activity in patients