Well-posedness, ill-posedness, and traveling waves for models of pulsatile flow in viscoelastic vessels

We study dispersive models of fluid flow in viscoelastic vessels, derived in the study of blood flow. The unknowns in the models are the velocity of the fluid in the axial direction and the displacement of the vessel wall from rest. We prove that one such model has a well-posed initial value problem, while we argue that a related model instead has an ill-posed initial value problem; in the second case, we still prove the existence of solutions in analytic function spaces. Finally we prove the existence of some periodic traveling waves.Comment: 28 pages, 1 figur

Phenomenological examinations of delirium in advanced cancer patients: exploratory structural equation modelling and latent profile analysis

Background This study examined phenomenological manifestations of delirium in advanced cancer patients by examining the factor structure of the Delirium Rating Scale-Revised-98 (DRS-R-98) and profiles of delirium symptoms. Methods Ninety-three patients with advanced cancer admitted to inpatient palliative care units in South Korea were examined by psychiatrists using the DRS-R-98 and the Confusion Assessment Method (CAM). The factor structure of the DRS-R-98 was examined by exploratory structural equation modelling analysis (ESEM) and profiles of delirium were examined by latent profile analysis (LPA). Results CAM-defined delirium was present in 66.6% (n = 62) of patients. Results from the ESEM analysis confirmed applicability of the core and noncore symptom factors of the DRS-R-98 to advanced cancer patients. LPA identified three distinct profiles of delirium characterizing the overall severity of delirium and its core and noncore symptoms. Class 1 (n = 55, 59.1%) showed low levels of all delirium symptoms. Class 2 (n = 17, 18.3%) showed high levels of core symptoms only, whereas Class 3 (n = 21, 22.6%) showed high levels of both core and noncore symptoms except motor retardation. Conclusions Clinical care for delirium in advanced cancer patients may benefit from consideration of the core and noncore symptom factor structure and the three distinct phenomenological profiles of delirium observed in the present study.This study was supported by the National Research and Development program for Cancer Control (HA17C0050), administered by the Ministry of Health & Welfare, Republic of Korea. The funding body had no role in the design of the study, the collection, analysis, and interpretation of data and in writing the manuscrip

An Interleukin-4 and Interleukin-13 Induced Atopic Dermatitis Human Skin Equivalent Model by a Skin-On-A-Chip

Currently, the mechanism of progression of atopic dermatitis (AD) is not well understood because there is no physiologically appropriate disease model in terms of disease complexity and multifactoriality. Type 2 inflammation, mediated by interleukin (IL)-4 and IL-13, plays an important role in AD. In this study, full-thickness human skin equivalents consisting of human-derived cells were fabricated from pumpless microfluidic chips and stimulated with IL-4 and IL-13. The morphological properties, gene expression, cytokine secretion and protein expression of the stimulated human skin equivalent (HSE) epidermis were investigated. The results showed epidermal and spongy formations similar to those observed in lesions in AD, and decreased expression of barrier-related filaggrin, loricrin and involucrin genes and proteins induced by IL-4Rα signaling. In addition, we induced the expression of carbonic anhydrase II (CAII), a gene specifically expressed in the epidermis of patients with AD. Thus, AD human skin equivalents can be used to mimic the key pathological features of atopic dermatitis, overcoming the limitations of existing studies that rely solely on mouse models and have been unable to translate their effects to humans. Our results will be useful for future research on the development of therapeutic agents for atopic dermatitis

Effects of Indole-3-Lactic Acid, a Metabolite of Tryptophan, on IL-4 and IL-13-Induced Human Skin-Equivalent Atopic Dermatitis Models

Indole-3-lactic acid (I3LA) is a well-known metabolite involved in tryptophan metabolism. Indole derivatives are involved in the differentiation of immune cells and the synthesis of cytokines via the aryl hydrocarbon receptors for modulating immunity, and the indole derivatives may be involved in allergic responses. I3LA was selected as a candidate substance for the treatment of atopic dermatitis (AD), and its inhibitory effect on AD progression was investigated. Full-thickness human skin equivalents (HSEs) consisting of human-derived cells were generated on microfluidic chips and stimulated with major AD-inducing factors. The induced AD-HSEs were treated with I3LA for 7 days, and this affected the AD-associated genetic biomarkers and increased the expression of the major constituent proteins of the skin barrier. After the treatment for 14 days, the surface became rough and sloughed off, and there was no significant difference between the increased AD-related mRNA expression and the skin barrier protein expression. Therefore, the short-term use of I3LA for approximately one week is considered to be effective in suppressing AD

Current Development and Applications of Super-Resolution Ultrasound Imaging

Abnormal changes of the microvasculature are reported to be key evidence of the development of several critical diseases, including cancer, progressive kidney disease, and atherosclerotic plaque. Super-resolution ultrasound imaging is an emerging technology that can identify the microvasculature noninvasively, with unprecedented spatial resolution beyond the acoustic diffraction limit. Therefore, it is a promising approach for diagnosing and monitoring the development of diseases. In this review, we introduce current super-resolution ultrasound imaging approaches and their preclinical applications on different animals and disease models. Future directions and challenges to overcome for clinical translations are also discussed

Current Development and Applications of Super-Resolution Ultrasound Imaging

Abnormal changes of the microvasculature are reported to be key evidence of the development of several critical diseases, including cancer, progressive kidney disease, and atherosclerotic plaque. Super-resolution ultrasound imaging is an emerging technology that can identify the mi-crovasculature noninvasively, with unprecedented spatial resolution beyond the acoustic diffraction limit. Therefore, it is a promising approach for diagnosing and monitoring the development of diseases. In this review, we introduce current super-resolution ultrasound imaging approaches and their preclinical applications on different animals and disease models. Future directions and challenges to overcome for clinical translations are also discussed. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.TRU

Effects of the Curing Conditions on the Carbonation Curing Efficiency of Ordinary Portland Cement and a Belite-Rich Cement Mortar

In the present study, the efficiency of five different carbonation and/or water curing conditions on the properties of belite-rich cement mortar and ordinary Portland cement mortar was investigated. The hybrid curing of samples was carried out by submerging samples at different levels in water or in a lime-saturated solution kept under carbonation curing conditions. The compressive strength was measured to compare the physical properties of the cement mortars, and X-ray diffraction and thermogravimetric analysis results were analyzed to compare the physicochemical properties. The results revealed that the supply of additional moisture during carbonation curing tends to decrease carbonation curing efficiency and that the hydration products of cement paste are predominantly affected by the depth at which the specimen was immersed in the liquid rather than the type of liquid used

Characteristics of Preplaced Aggregate Concrete Fabricated with Alkali-Activated Slag/Fly Ash Cements

This study assesses the characteristics of preplaced aggregate concrete prepared with alkali-activated cement grout as an adhesive binder. Various binary blends of slag and fly ash without fine aggregate as a filler material were considered along with different solution-to-solid ratios. The properties of fresh and hardened grout along with the properties of hardened preplaced concrete were investigated, as were the compressive strength, ultrasonic pulse velocity, density, water absorption and total voids of the preplaced concrete. The results indicated that alkali-activated cement grout has better flowability characteristics and compressive strength than conventional cement grout. As a result, the mechanical performance of the preplaced aggregate concrete was significantly improved. The results pertaining to the water absorption and porosity revealed that the alkali-activated preplaced aggregate concrete is more resistant to water permeation. The filling capacity based on the ultrasonic pulse velocity value is discussed to comment on the wrapping ability of alkali-activated cement grout

Rhamnetin, a Natural Flavonoid, Ameliorates Organ Damage in a Mouse Model of Carbapenem-Resistant Acinetobacter baumannii-Induced Sepsis

In sepsis, the persistence of uncontrolled inflammatory response of infected host cells eventually leads to severe lung and organ failure and, ultimately, death. Carbapenem-resistant Acinetobacter baumannii (CRAB), causative bacteria of sepsis and lung failure in acute cases, belongs to a group of critical pathogens that cannot be eradicated using the currently available antibiotics. This underlines the necessity of developing new modes of therapeutics that can control sepsis at the initial stages. In this study, we investigated the anti-inflammatory activities in vitro and in vivo and the antiseptic effects of rhamnetin, a naturally occurring flavonoid. We found that among its isoforms, the potency of rhamnetin was less explored but rhamnetin possessed superior anti-inflammatory activity with least cytotoxicity. Rhamnetin showed significant anti-inflammatory effects in lipopolysaccharide-, CRAB-, and Escherichia coli (E. coli)-stimulated mouse macrophages by inhibiting the release of interleukin-6 and nitric oxide. In a mouse model of sepsis infected with clinically isolated CRAB or E. coli, rhamnetin significantly reduced the bacterial burden in the organs. In addition, normalized pro-inflammatory cytokine levels in lung lysates and histological analysis of lung tissue indicated alleviation of lung damage. This study implies that a potent natural product such as rhamnetin could be a future therapeutic for treating carbapenem-resistant gram-negative sepsis

Antioxidant and Anti-Inflammatory Effects of <i>Agarum cribrosum</i> Extract and Its Fractions in LPS-Induced RAW 264.7 Macrophages

Excessive oxidative stress and chronic inflammation are implicated in the pathogenesis of chronic inflammation diseases. The purpose of this study was to determine whether the antioxidant and anti-inflammatory effects of Busan-grown Agarum cribrosum ethanol extract (ACE) and its organic solvent five fractions are exhibited in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. ACE and its five fractions from ACE evaluated the total antioxidant activity and the effect of pro-inflammatory cytokines and antioxidant genes expression in LPS-induced RAW 264.7 macrophages. ACE and its ethyl acetate (EtOAc) fraction showed a high total phenolic content and total antioxidant capacity by decreasing free radicals scavenging activity. ACE and its EtOAc fraction significantly repressed LPS-induced tumor necrosis factor α and interleukin-1β gene expression. Additionally, ACE and its EtOAc fraction significantly diminished the LPS-stimulated gene expression of inducible nitric oxide synthase and cyclooxygenase 2 genes with a concomitant decrease in their protein levels in the macrophages. The gene expression of NADPH oxidase 2 was significantly abolished by ACE and its EtOAc fraction in LPS-induced macrophages, while other antioxidant genes showed minimal effects. The results suggest that ACE and its EtOAc fraction exert inhibitory effects on LPS-stimulated inflammation and oxidative stress in macrophages accompanied by total antioxidant activity