83 research outputs found
Nearly strain-free heteroepitaxial system for fundamental studies of pulsed laser deposition: EuTiO3 on SrTiO3
High quality epitaxial thin-films of EuTiO3 have been grown on the (001)
surface of SrTiO3 using pulsed laser deposition. In situ x-ray reflectivity
measurements reveal that the growth is two-dimensional and enable real-time
monitoring of the film thickness and roughness during growth. The film
thickness, surface mosaic, surface roughness, and strain were characterized in
detail using ex situ x-ray diffraction. The thicnkess and composition were
confirmed with Rutherford Backscattering. The EuTiO3 films grow
two-dimensionally, epitaxially, pseudomorphically, with no measurable in-plane
lattice mismatch.Comment: 7 pages, 6 figure
Dynamics of oscillating scalar field in thermal environment
There often appear coherently oscillating scalar fields in particle physics
motivated cosmological scenarios, which may have rich phenomenological
consequences. Scalar fields should somehow interact with background thermal
bath in order to decay into radiation at an appropriate epoch, but introducing
some couplings to the scalar field makes the dynamics complicated. We
investigate in detail the dynamics of a coherently oscillating scalar field,
which has renormalizable couplings to another field interacting with thermal
background. The scalar field dynamics and its resultant abundance are
significantly modified by taking account of following effects : (1) thermal
correction to the effective potential, (2) dissipation effect on the scalar
field in thermal bath, (3) non-perturbative particle production events and (4)
formation of non-topological solitons. There appear many time scales depending
on the scalar mass, amplitude, couplings and the background temperature, which
make the efficiencies of these effects non-trivial.Comment: 45 pages, 6 figures; v2: several typos corrected; v3: minor
corrections and references added; v4: minor corrections to reflect the
published version; v5: minor correction
Two-dimensional capillary zone electrophoresis–mass spectrometry for the characterization of intact monoclonal antibody charge variants, including deamidation products.
Capillary zone electrophoresis (CZE) is a powerful tool that is progressively being applied for the separation of monoclonal antibody (mAb) charge variants. Mass spectrometry (MS) is the desired detection method concerning identification of mAb variants. In biopharmaceutical applications, there exist optimized and validated electrolyte systems for mAb variant quantification. However, these electrolytes interfere greatly with the electrospray ionization (ESI) process. Here, a heart-cut CZE–CZE–MS setup with an implemented mechanical four-port valve interface was developed that used a generic ε-aminocaproic acid based background electrolyte in the first dimension and acetic acid in the second dimension. Interference-free, highly precise mass data (deviation less than 1 Da) of charge variants of trastuzumab, acting as model mAb system, were achieved. The mass accuracy obtained (low parts per million range) is discussed regarding both measured and calculated masses. Deamidation was detected for the intact model antibody, and related mass differences were significantly confirmed on the deglycosylated level. The CZE–CZE–MS setup is expected to be applicable to a variety of antibodies and electrolyte systems. Thus, it has the potential to become a compelling tool for MS characterization of antibody variants separated in ESI-interfering electrolytes
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