Classical swine fever and foot-and-mouth disease in Lao PDR

Approximately 75% of the population of Lao PDR is engaged in agriculture and the vast majority (approximately 90%) of these producers are in the smallholder sector. Livestock are an important contributor to national, agricultural and village economies and are relied on for food security. The pig population has increased over the past 5 years at an annual average increase of 4.7% at the national herd level and up to 20% in some provinces. Cattle and buffalo populations have grown at more modest rates of 1–2% (Figure 1). Disease, including foot-and-mouth disease (FMD) and classical swine fever (CSF), is a major constraint to efficient and sustainable livestock production. Up to 80–90% of pigs and 99% of cattle and buffalo are produced in the smallholder sector using low input practices; as such, there is limited private sector input. Disease reporting, diagnosis, control and prevention are addressed by the Lao Government through the National Department of Livestock and Fisheries (DLF) and local agriculture and forestry offices at provincial and district government levels. These activities are supported by international partners such as the Australian Centre for International Agricultural Research (ACIAR), Commonwealth Scientific and Investigation Research Organisation (CSIRO), Japanese International Cooperation Association (JICA), Food and Agriculture Organization (FAO), European Union (EU) and Office International des Epizooties (OIE). Disease reporting and communication are passive and reports are made from villages through government administrations at district and provincial levels and then to the national level—the DLF and the National Animal Health Centre (NAHC). Communication of FMD-related information at regional and international levels is coordinated by the OIE South-East Asian FMD regional coordination unit (SEAFMD RCU), where reports are submitted monthly. Disease reporting for CSF is less well coordinated and information is provided to the OIE

Improving Trade in Large Ruminants and Products by Transboundary Animal Disease Control in Lao PDR

Within the Greater Mekong Subregion (GMS) the nation of Lao PDR has a small population of ~6.3 million people and a relatively large population of large ruminants (cattle and buffalo) at ~2.7 million head. With the growing demand for red meat in South-East Asia driven by a rising middle class and the associated changes in dietary intake, Lao smallholder farmers have the opportunity to satisfy this demand provided key constraints are addressed. Recent research has highlighted a series of best practice interventions directed at the smallholder level to improve animal health and production. Animal movement and trade have been identified as a major risk factor involved in transboundary animal disease (TAD) transmission including foot and mouth disease. Hence, understanding the supply chain is important for effective TAD control. The results of a survey of 32 large ruminant traders in northern Laos in 2011 were matched to a longitudinal production survey from 6 villages in northern Laos to develop a value chain analysis. The 32 traders provided details on 8,796 large ruminant trades, operating locations, large ruminant purchase prices, transport methods, major costs, livestock destinations and trader views on major constraints to development of the large ruminant market. The 2011 farm gate value of the national large ruminant herd was estimated as USD 835.8 million based on trader purchase price and village herd production data. As improvement of large ruminant production has been linked to reducing regional rural poverty and food insecurity in smallholder communities through opportunities for business development and rural employment, addressing both TADs and the underdeveloped market in the GMS is important. Whilst control of TADs will need to remain a medium term priority, further research is needed to ensure that market development remains aligned with disease control efforts

A Bayesian network model to explore practice change by smallholder rice farmers in Lao PDR

© 2018 A Bayesian Network model has been developed that synthesizes findings from concurrent multi-disciplinary research activities. The model describes the many factors that impact on the chances of a smallholder farmer adopting a proposed change to farming practices. The model, when applied to four different proposed technologies, generated insights into the factors that have the greatest influence on adoption rates. Behavioural motivations for change are highly dependent on farmers' individual viewpoints and are also technology dependent. The model provides a boundary object that provides an opportunity to engage experts and other stakeholders in discussions about their assessment of the technology adoption process, and the opportunities, barriers and constraints faced by smallholder farmers when considering whether to adopt a technology

Seroprevalence of major bovine-associated zoonotic infectious diseases in the Lao People's Democratic Republic

Bovine-associated zoonotic infectious diseases pose a significant threat to human health in the Lao People's Democratic Republic (Lao PDR). In all, 905 cattle and buffalo serum samples collected in northern Lao PDR in 2006 were used to determine seroprevalence of five major bovine zoonotic infectious diseases that included Taenia saginata cysticercosis, bovine tuberculosis, Q-fever, bovine brucellosis, and bovine leptospirosis. Five enzyme-linked immunosorbent assays (ELISAs) were used to test for the presence of antibodies to the diseases, except Taenia saginata, for which we tested for the presence of Taenia metacestode circulating antigens. The overall highest prevalence was for T. saginata (46.4%), with lower prevalence for Q-fever (4%), leptospirosis (3%), tuberculosis (1%), and brucellosis (0.2%). Although there were no significant differences in the proportion of seroprevalence between sex and age of the animals sampled, there were significant differences between the provincial distributions. Further studies are required to determine the seroprevalence of these infections in other locations in Lao PDR, as well as other animal species including humans, in order to develop effective prevention and control strategies. This is the first study to investigate the prevalence of bovine zoonotic infectious agents in the Lao PDR. Positivity was demonstrated for all diseases investigated, with the highest prevalence for T. saginata antigen and Coxiella burnetti antibodies. For T. saginata, there were significant differences in the provincial distribution. Approximately 16% seroprevalence of Coxiella burnetti was noted in Xayabuly Province; however, there are no clear reasons why this was the case, and further studies are required to determine risk factors associated with this observation

Recommended vaccine programs for village-based pig production systems in Lao PDR

Classical swine fever (CSF) virus is endemic in Lao PDR, with major outbreaks each year resulting in significant production losses in all farming systems, including smallholder, semi-intensive and intensive farms. CSF is a vaccine-preventable disease and there are many vaccines, both live attenuated and subunit, commercially available. In Lao PDR a variety of livestock vaccines are produced at the National Vaccine Production Centre (NVPC), which is located at Nongteng village 15 km from Vientiane. The NVPC was established in 1980 and produces vaccines for CSF, haemorrhagic septicaemia, Newcastle disease, fowl cholera, infectious bronchitis, fowl pox and duck plague. CSF vaccine is produced from the live attenuated C-strain virus from homogenised rabbit spleen and mesenteric lymph nodes freeze dried in the presence of stabilisers in a rubber stoppered vial and stored at –20 °C

A simple and inexpensive container for the transport of biological specimens in limited resource situations.

We describe a diagnostic specimen transport container that is appropriate for limited resource or emergency settings. The transport container is constructed from polyvinyl chloride (PVC) plumbing pipe, which is readily available and inexpensive (US$1-2, depending on size) and has wide flexibility of size due to the range of PVC pipe dimensions available. The PVC transporters are durable, water-resistant and may be easily decontaminated. They have been adapted for the transport of blood culture bottles from provincial hospitals in Laos, where, during a 2-year period, 380 PVC tubes containing blood culture bottles were transported without any leakage or breakage. We have found the PVC transporter to be a useful and cost-efficient durable alternative that meets IATA Packing Instruction 650 biological transport container requirements

Classical swine fever virus vaccine stability in Lao PDR

Classical swine fever (CSF) virus is a highly contagious but vaccine-preventable disease of swine. A locally produced lapinised C-strain vaccine is used to control CSF in Lao PDR; however, vaccine failure has been reported. The CSF vaccine is produced at the National Vaccine Production Centre (NVPC) as a freeze-dried rabbit spleen homogenate in a rubber stoppered glass vial and stored at –20 °C with a recommended shelf life of 1 year. This paper describes two studies to (i) determine the stability of the locally produced vaccine when stored at 4 °C and –20 °C and (ii) determine if the vaccine elicits a protective immune response when delivered to village pigs under good transport conditions. The vaccine was found to be stable for only 4 months when stored at –20 °C and for less than 3 months when stored at 4 °C. Under field conditions, vaccine stored at –20 °C for 2 months and transported at temperatures less than 1 °C elicited an immune response in 89% of vaccinated pigs by day 35 and 100% of pigs by day 70 post vaccination