A modified pulsed-field gel electrophoresis (PFGE) protocol was developed and applied to clinical isolates of Mycobacterium tuberculosis complex to reduce the cost of using lyticase. This protocol reduces the expense of PFGE typing of Mycobacterium tuberculosis complex as it removes the use of lyticase during the spheroplast formation from these bacteri

Darban Sarokhalil, D.

Feizabadi, M.

Fooladi, A.

Ghodousi, A.

Khosaravi, A.

Omrani, M.

Vatani, S.

PubMed Central

Archivio istituzionale della ricerca - Università di Palermo

DNA polymorphisms in strains of Mycobacterium tuberculosis analyzed by pulsed-field gel electrophoresis: a tool for epidemiology.

Genomic analysis of Mycobacterium bovis and other members of the Mycobacterium tuberculosis complex by isoenzyme analysis and pulsed-field gel electrophoresis.

GIobal epidemiology of tuberculosis : morbidity and mortality of a worldwide epidemic. JAMA

Improved preparation of high molecular weight DNA for pulsed-field gel electrophoresis from mycobacteria.

Physical mapping of Mycobacterium bovis BCG Pasteur reveals differences from the genome map of

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Shahcheraghi F: A novel DNA extraction method for PFGE analysis of Staphylococcus aureus and enterococci without using lysostaphin.

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Development of a new DNA extraction protocol for PFGE typing of Mycobacterium tuberculosis complex.