Indole-3-acetic acid is produced by Emiliania huxleyi coccolith-bearing cells and triggers a physiological response in bald cells

© 2016 Labeeuw, Khey, Bramucci, Atwal, de la Mata, Harynuk and Case. Indole-3-acetic acid (IAA) is an auxin produced by terrestrial plants which influences development through a variety of cellular mechanisms, such as altering cell orientation, organ development, fertility, and cell elongation. IAA is also produced by bacterial pathogens and symbionts of plants and algae, allowing them to manipulate growth and development of their host. They do so by either producing excess exogenous IAA or hijacking the IAA biosynthesis pathway of their host. The endogenous production of IAA by algae remains contentious. Using Emiliania huxleyi, a globally abundant marine haptophyte, we investigated the presence and potential role of IAA in algae. Homologs of genes involved in several tryptophan-dependent IAA biosynthesis pathways were identified in E. huxleyi. This suggests that this haptophyte can synthesize IAA using various precursors derived from tryptophan. Addition of L-tryptophan to E. huxleyi stimulated IAA production, which could be detected using Salkowski's reagent and GC × GC-TOFMS in the C cell type (coccolith bearing), but not in the N cell type (bald). Various concentrations of IAA were exogenously added to these two cell types to identify a physiological response in E. huxleyi. The N cell type, which did not produce IAA, was more sensitive to it, showing an increased variation in cell size, membrane permeability, and a corresponding increase in the photosynthetic potential quantum yield of Photosystem II (PSII). A roseobacter (bacteria commonly associated with E. huxleyi) Ruegeria sp. R11, previously shown to produce IAA, was co-cultured with E. huxleyi C and N cells. IAA could not be detected from these co-cultures, and even when stimulated by addition of L-tryptophan, they produced less IAA than axenic C type culture similarly induced. This suggests that IAA plays a novel role signaling between different E. huxleyi cell types, rather than between a bacteria and its algal host

Fighting America’s Highest Incarceration Rates with Offender Programming: Process Evaluation Implications from the Louisiana 22nd Judicial District Reentry Court

Reentry programs, when adequately funded and delivered with fidelity, can render recidivism reduction and other positive outcomes such as abstinence and employment stability. This paper reports process evaluation findings for the Louisiana 22nd Judicial District Reentry Court program, a joint SAMHSA/BJA-sponsored multiphase programming intervention for high-risk/high-need offenders featuring job readiness training in the Louisiana State Penitentiary at Angola and transition services during reentry, including program engagement, job placement, and treatment services continuation in the community under strict judicial supervision. Research procedures entailed 1) observation of court appearances, treatment team meetings, educational activities, and counseling sessions, 2) review of all program participant case files enabling progress tracking, and 3) in-depth and focus group interviews with program stakeholders both at Angola and post-release in community settings. Findings relate the evidence based nature and quality of services delivery to date, as well as fidelity demonstrated across major programmatic domains. Program improvement opportunities, outcome evaluation implications, and performance measures signaling early success center discussion around vanguard elements of the court and evaluation design, respectively

An Implementation and Process Evaluation of the Louisiana 22nd Judicial District’s Behavioral Health Court

The national offender reentry movement, Second Chance Act, and the widespread transfer of offender programming to community corrections have coalesced to substantially increase treatment for mental health and substance disorders within the criminal justice system. Intervention commonly entails program evaluation for accountability and empirical evidence by which to specify what works. Though mixed methods evaluation is preferable to a singular qualitative or quantitative approach, process steps are commonly overlooked. This paper relates an implementation and process design and evaluation midpoint findings for the Louisiana 22nd Judicial District’s Behavioral Health Court program, a post-conviction treatment initiative for mental health offenders. Interview guides and a fidelity instrument facilitated site visit data collection. Findings inform program implementation intensity, performance, improvement opportunities, and related fidelity research

Geminiviruses : genome organization and protein functions

International audienc

Single crystal structure, solid state characterization and dissolution rate of terbinafine hydrochloride

Terbinafine hydrochloride (TH), a poorly water soluble antifungal agent, was characterized by solid state techniques including differential scanning calorimetry, thermogravimetry, X-ray powder diffraction, optical and electron microscopies, Fourier transform infrared, Raman and solid-state nuclear magnetic resonance spectroscopies and intrinsic dissolution rate (IDR). A colorless single crystal of TH was grown from an ethanol:water solution and its crystalline structure was determined through X-ray single crystal diffraction. Also, a new crystal habit of TH was obtained through the slow solvent evaporation technique revealing a needle-like shape. A comparison between the IDR results for the TH raw material and TH needle-like crystal revealed lower values for the new crystal habit, which can be attributed to the preferential orientation of the crystals in the compressed disks.Fil: Kuminek, Gislaine. Universidade Federal de Santa Catarina; BrasilFil: Rauber, Gabriela Schneider. Universidade Federal de Santa Catarina; BrasilFil: Riekes, Manoela Klüppel. Universidade Federal de Santa Catarina; BrasilFil: Campos, Carlos Eduardo Maduro de. Universidade Federal de Santa Catarina; BrasilFil: Monti, Gustavo Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Física Enrique Gaviola. Universidad Nacional de Córdoba. Instituto de Física Enrique Gaviola; ArgentinaFil: Bortoluzzi, Adailton João. Universidade Federal de Santa Catarina; BrasilFil: Cuffini, Silvia Lucia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidade Federal de Santa Catarina; BrasilFil: Cardoso, Simone Goncalves. Universidade Federal de Santa Catarina; Brasi

Geminiviruses : genome organization and protein functions

International audienc