Učinci konjske rotkvice (Moringa oleifera) kao dodatka prehrani na antioksidacijski status junica križanki različitih pasmina

The experiment was conducted to evaluate the anti-oxidant effect of Moringa oleifera meal-supplementation in the total mixed ration, replacing the compounded concentrate mixture for crossbred heifer calves. A total of twenty-one crossbred heifer calves were selected and grouped into three treatments based on body weight and age. The leaves (84%) and soft twigs (16%) of Moringa oleifera were mixed to prepare the Moringa oleifera meal. Compounded concentrate mixture, ground nut straw, hybrid napier grass, mineral mixture and common salt were used at 50, 23, 25, 1 and 1%, respectively to formulate the control total mixed ration (T1). The compounded concentrate mixture was replaced with the Moringa oleifera meal at 5.0 and 7.5 % to formulate total mixed rations T2 and T3. The whole blood was collected from each calves in the morning before feeding and watering at the beginning (0 day), middle (at 60 day) and end (at 126 day) of the experiment. The total leukocyte counts, total erythrocyte counts, haematocrit and platelet count of crossbred heifer calves differed non-significantly (P>0.05) between the treatment groups, and were within normal physiological range. The serum albumin, glucose, creatinine, urea, alkaline phosphatase, alanine amino transaminase, calcium and phosphorus differed non-significantly between the treatment groups. However, feeding with Moringa oleifera resulted in higher (P0.05) higher after feeding with the 5.0 and 7.5% Moringa oleifera meals. Feeding with 5.0 and 7.5% Moringa oleifera meal in the total mixed ration, replacing the high protein compounded concentrate mixture, to growing crossbred heifer calves had no adverse effect on haematological and biochemical constituents, and resulted in a non-significant (P>0.05) higher antioxidant capacity in the crossbred heifer calves.Ovo je istraživanje provedeno kako bi se procijenio antioksidacijski učinak konjske rotkvice (Moringa oleifera) kao zamjene za mješavinu koncentrata u hranidbi junica križanki različitih pasmina. Na temelju tjelesne mase i dobi, junice (n=21) su razvrstane u tri pokusne skupine. Lišće (84%) i mekše grančice (16%) konjske rotkvice izmiješane su kako bi se pripremio obrok za istraživane životinje. U formulaciji mješavine (T1) upotrijebljena je mješavina koncentrata u količini od 50%, mljeveni orašasti plodovi 23%, Hybrid Napier 25%, mineralna mješavina 1 % i obična sol 1 %. Pripreljena smjesa koncentrata zamijenjena je obrokom s konjskom rotkvicom u omjeru 5,0% i 7,5% za skupine T2 i T3. Od svake je junice uzet uzorak pune krvi ujutro prije hranjenja i pojenja na početku (0. dan), sredinom (60. dan) i na kraju pokusa (126. dan). Nije bilo statistički znakovite razlike (P>0,05) u ukupnom broju leukocita i eritrocita, hematokritu te broju trombocita među pokusnim skupinama i vrijednosti su bile unutar fiziološkog raspona. Ni u vrijednostima serumskog albumina, glukoze, kreatinina, ureje, alkalne fosfataze, alanin-aminotransaminaze, kalcija i fosfora nije bilo statistički znakovite razlike među pokusnim skupinama. Dodatak prehrani konjske rotkvice međutim rezultirao je porastom proteina u serumu (P0,05) pri hranjenju obrocima s dodatkom konjske rotkvice. Prehrana s 5,0 i 7,5% dodatka konjske rotkvice koja je zamijenila visokoproteinsku koncntriranu mješavinu u obrocima junica nije utjecala na njihove hematološke i biokemnijske pokazatelja kao ni na njihov antioksidacijski kapacitet čije neznatno povećanje nije bilo statistički znakovito (P>0,05)