A születési súly hatása az ivarérésre és a szaporodásbiológiai teljesítményre keresztezett Moo Lath x Duroc kocasüldőknél

This study aims to evaluate the effects of the birth weight of crossbred Moo Lath x Duroc (CMD) gilts on their puberty and first mating age, including the body condition and morphology. The litter size and birth weight of their offspring were also evaluated. Eighteen (18) CMD gilts were selected after weaning and kept in an individual pen 1.5 x 2 x 1 m after puberty. The gilts were grouped into three groups based on birth weight: groups A, B, and C (with 0.9 kg, respectively). 15 mature purebred Moo Lath (PML) gilts reared by farmers were also involved in this study to compare the morphology between the CMD and PML at first mating. There was no difference in the age and body weight of CMD gilts at puberty and first mating among the studied groups. However, the gilts in group A showed a lower mean age at puberty, and marginally lighter body weight than those in groups B and C. Birth weight showed a significant influence on the backfat thickness at puberty and first mating (P0.9 kg). Tizenöt (15) tenyészérett Moo Lath (PML) farmon nevelt süldőt is értékeltünk a vizsgálatban, hogy összehasonlítsuk az először filat CMD illetve PML süldők küllemét. Korban és testsúlyban nem volt szignifikáns különbség a vizsgált CMD süldők között ivarérettség idején, illetve az első termékenyítéskor. Ugyanakkor az A csoport egyedeinek valamivel alacsonyabb volt az átlagéletkora és súlya ivaréréskor mint a B és C csoport süldőinek. A születési súly szignifikánsan befolyásolta ivaréréskor és az első termékenyítés idején a hátszalonna vastagságot (P<0.039 és 0.031). A CMD süldők első termékenyítése 193 naposan történt a harmadik-negyedik ivarzásuk után, körülbelül 40 kg os súlyban, 38 mm-es hátszlonna vastagságnál, 90 cm-es övmérettel, 100 cm-es testhossznál, és 51 cm-es marmagasságnál. A CMD süldők születési súlya nem befolyásolta az alomméretüket, de hatással volt a malacaik születési súlyára. Az első termékenyítéskori testsúlyban nem volt szignifikáns különbség a CMD és PML süldők között, ugyanakkoraz átlagos testhosszuk és övméretük a CMD süldőknek valamivel nagyobb volt

Preliminary results of the recombinase polymerase amplification technique for the detection of Haemonchus contortus from Hungarian field samples

Haemonchus contortus is a parasitic nematode of small ruminants responsible for significant economic losses and animal health concerns globally. Detection of gastrointestinal nematode (GIN) infection in veterinary practice typically relies on microscopy-based methods such as the faecal egg count and morphological identification of larval culture. However, mixed co-infections are common and species-specific identification is typically time-consuming and expertise-intensive. Compounded by increasing anthelmintic resistance, there is an urgent need to implement the molecular diagnosis of GIN in the livestock industry, preferably in field settings. Advances in isothermal amplification techniques including recombinase polymerase amplification (RPA) assays could improve this. Yet, constraints in RPA kit availability and amplicon detection systems limit the use of this technology in point of care settings. In this study, we present an early-stage, proof-of-concept demonstration of RPA targeting the internal transcribed spacer (ITS2) region of H. contortus. Having tested against eight closely related nematodes and also against five farm isolates in Eastern Hungary, preliminary results derived from a comparative analysis of 3 primer sets showed the assay detects H. contortus DNA and has a limit of detection of 10 ng/μl. We also tested an end-result naked eye detection system using various DNA binding dyes, of which EvaGreen® dye was successful for a qualitative RPA detection that could be adaptable at farm sites. [Abstract copyright: Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.

Point of care colourimetric and lateral flow LAMP assay for the detection of

In this study, we present an optimised colourimetric and a lateral flow LAMP assay for the detection of Haemonchus contortus in small ruminant faecal samples. Using a previously published LAMP primer set, we made use of commercially available colourimetric LAMP and lateral flow kits and combined this into an optimised diagnostic assay which was then tested on field faecal samples from Eastern and South-Eastern Hungary as well as a pure H. contortus egg faecal sample from Košice, Slovakia. Both assays showed no conflicts in visual detection of the results. Additionally, we modified and tested several centrifuge-free DNA extraction methods and one bead-beating egg lysis DNA extraction method to develop a true point of care protocol, as the source of the starting DNA is the main rate-limiting step in farm-level molecular diagnosis. Out of the various methods trialed, promising results were obtained with the magnetic bead extraction method. Sample solutions from the Fill-FLOTAC® technique were also utilised, which demonstrated that it could be efficiently adapted for field-level egg concentration to extract DNA. This proof of concept study showed that isothermal amplification technologies with a colourimetric detection or when combined with a lateral flow assay could be an important step for a true point of care molecular diagnostic assay for H. contortus