Profile of paediatric low vision population: a retrospective study from Nepal.

BACKGROUND: Childhood blindness and low vision have become major public health problems in developing countries. The purpose of this study was to categorise the causes of visual impairment according to aetiology and provide detailed local information on visually impaired children seeking low-vision services in a tertiary eye centre in Nepal. METHODS: A retrospective study was conducted of all visually impaired children (visual acuity of less than 6/18 in the better eye), aged less than 17 years seen in the low-vision clinic at the Sagarmatha Chaudhary Eye Hospital in Lahan between January 1, 2012 and December 31, 2013. RESULTS: Of the 558 visually impaired children, the majority were males, 356 (63.7 per cent). More than half (56.5 per cent) of the children were in the 11 to 16 years age group. Many of the low-vision children (52.9 per cent) were identified as having moderate visual impairment (visual acuity less than 6/18 to 6/60). Most children were diagnosed with childhood (36.2 per cent) or genetic (35.5 per cent) aetiology, followed by prenatal (22.2 per cent) and perinatal (6.1 per cent) aetiologies. Refractive error and amblyopia (20.1 per cent), retinitis pigmentosa (14.9 per cent) and macular dystrophy (13.4 per cent) were the most common causes of paediatric visual impairment. Nystagmus (50.0 per cent) was the most common cause of low vision in the one to five years age group, whereas refractive error and amblyopia were the major causes in the six to 10 and 11 to 16 years age group (17.6 and 22.9 per cent, respectively). Many of the children (86.0 per cent) were prescribed low-vision aids and 72.0 per cent of the low-vision aid users showed an improvement in visual acuity either at distance or near. CONCLUSION: Paediatric low vision has a negative impact on the quality of life in children. Data from this study indicate that knowledge about the local characteristics and aetiological categorisation of the causes of low vision are essential in tackling paediatric visual impairment. The findings also signify the importance of early intervention to ensure a better quality of life

1st International Conference on Bioresource Technology for Bioenergy, Bioproducts & Environmental Sustainability (BIORESTEC)

With growing global interest in bioenergy, biobased product and environmental sustainability, the first International Conference on Bioresource Technology for Bioenergy, Bioproducts & Environmental Sustainability (BIORESTEC) was organized from October 2326, 2016 in Sitges, Barcelona in Spain. The conference was organized in association with Elseviers premier journal Bioresource Technology (BITE), with an aim to provide a shared forum for researchers, academicians, industries, and policymakers, to discuss the current state-of-the-art and the emerging trends in biotechnology, bioenergy, and biobased products. The 1st BIORESTEC conference received tremendous response from all over the globe with 754 abstracts submitted. The scientific committee consisted of 13 eminent scientists from 11 countries. The committee then screened and selected 54 abstracts for oral and 166 abtsracts for poster presentations. Besides, there were 19 invited speakers from 14 countries. Apart from the scientific presentations, a workshop on How to write a scientific paper and get published was also organized for early career researchers by Elsevier. This special issue of the journal contain 29 papers (all presented at the BIORESTEC conference) after peer-review process. These papers broadly cover areas such as biomass pretreatment, algal and lignocellulose biorefinery, biological waste treatment, white biotechnology and biomass policies, LCA and techno-economics and classified as below.info:eu-repo/semantics/publishedVersio

Ultrasonication of Sugary -2 Corn for Enhanced Enzymatic Hydrolysis

This study investigates the potential application of high powered ultrasonics as a liquefaction pretreatment of sugary-2 corn slurry. Ground sugary-2 corn ( Zea Mays L. ) slurry was treated with ultrasonics at 20kHz and amplitudes of 192-320 µ m pp (peak-to-peak) for 5, 10, 15, 20 and 40 seconds. After sonication, enzymes (Stargen TM 001) were added to the samples to hydrolyze the starch into fermentable sugars. It was found that the reducing sugar released in the treated samples were 6-fold higher than in the non-treated samples. Scanning electron microscopy images revealed that the sugary starch was partially gelatinized during sonication. This observation was confirmed by polarized-light microscopic images, where deformed “Maltese crosses” were found. The swelling rate of sonicated samples was nearly 66 times higher than when applying conventional heating. This result confirms better gelatinization capability of ultrasonics compared to conventional heating. The maximum relative net energy gain (additional chemically released energy) of the sonicated samples was at 5s of sonication time with a power setting between 248-330W. The findings in this study indicated ultrasonics as a promising pretreatment step in sugary-2 corn hydrolysis

Enzyme Production by Wood-Rot and Soft-Rot Fungi Cultivated on Corn Fiber Followed by Simultaneous Saccharification and Fermentation

This research aims at developing a biorefinery platform to convert lignocellulosic corn fiber into fermentable sugars at a moderate temperature (37 °C) with minimal use of chemicals. White-rot (Phanerochaete chrysosporium), brown-rot (Gloeophyllum trabeum), and soft-rot (Trichoderma reesei) fungi were used for in situ enzyme production to hydrolyze cellulosic and hemicellulosic components of corn fiber into fermentable sugars. Solid-substrate fermentation of corn fiber by either white- or brown-rot fungi followed by simultaneous saccharification and fermentation (SSF) with coculture of Saccharomyces cerevisiae has shown a possibility of enhancing wood rot saccharification of corn fiber for ethanol fermentation. The laboratory-scale fungal saccharification and fermentation process incorporated in situ cellulolytic enzyme induction, which enhanced overall enzymatic hydrolysis of hemi/cellulose components of corn fiber into simple sugars (mono-, di-, and trisaccharides). The yeast fermentation of the hydrolyzate yielded 7.8, 8.6, and 4.9 g ethanol per 100 g corn fiber when saccharified with the white-, brown-, and soft-rot fungi, respectively. The highest ethanol yield (8.6 g ethanol per 100 g initial corn fiber) is equivalent to 35% of the theoretical ethanol yield from starch and cellulose in corn fiber. This research has significant commercial potential to increase net ethanol production per bushel of corn through the utilization of corn fiber. There is also a great research opportunity to evaluate the remaining biomass residue (enriched with fungal protein) as animal feed

Solid-Substrate Fermentation of Corn Fiber by Phanerochaete chrysosporium and Subsequent Fermentation of Hydrolysate into Ethanol

The goal of this study was to develop a fungal process for ethanol production from corn fiber. Laboratory-scale solid-substrate fermentation was performed using the white-rot fungusPhanerochaete chrysosporium in 1 L polypropylene bottles as reactors via incubation at 37 °C for up to 3 days. Extracellular enzymes produced in situ by P. chrysosporium degraded lignin and enhanced saccharification of polysaccharides in corn fiber. The percentage biomass weight loss and Klason lignin reduction were 34 and 41%, respectively. Anaerobic incubation at 37 °C following 2 day incubation reduced the fungal sugar consumption and enhanced the in situ cellulolytic enzyme activities. Two days of aerobic solid-substrate fermentation of corn fiber with P. chrysosporium, followed by anaerobic static submerged-culture fermentation resulted in 1.7 g of ethanol/100 g of corn fiber in 6 days, whereas yeast (Saccharomyces cerevisiae) cocultured with P. chrysosporium demonstrated enhanced ethanol production of 3 g of ethanol/100 g of corn fiber. Specific enzyme activity assays suggested starch and hemi/cellulose contribution of fermentable sugar

Ultrasonic Pretreatment of Corn Slurry in Batch and Continuous Systems

The effects of ultrasonication of corn slurry, on particle size distribution and enzymatic hydrolysis was studied for the dry-grind mill ethanol industry. Two independent ultrasonic experiments were conducted at a frequency of 20 kHz; in batch and continuous systems. The ground corn slurry (33% m/v) was pumped at flow rates 10-28 L/min in continuous flow experiments, and sonicated at constant amplitude (20µmpeak-to-peak(p-p)). Ultrasonic batch experiments were conducted at varying amplitudes of 192-320µmp-p. After ultrasonication, StargenTM001 enzyme was added to the samples and a short 3h hydrolysis followed. The treated samples were found to yield 2-3 times more reducing sugar compared to the control (untreated) samples. In terms of energy density, the batch ultrasonic system was found to deliver 25-times more energy than the continuous flow systems. Although the experiments conducted in continuous system released less reducing sugar than the batch system, the continuous system was more energy efficient. The particle size of the sonicated corn slurry (both batch and continuous) was reduced relative to the controls (without treatment). The reduction of particle size was directly proportional to the energy input during sonication. The study suggests that both batch and continuous flow ultrasonic systems enhances enzymatic hydrolysis yield, reduces particle size of corn slurry and could be a potential effective pretreatment for corn slurry