Inheritance of plumage colour in Japanese quails

A complete 4 × 4 diallel cross involving 4 quail populations, 2 of which possessed Pharao, plumage and other 2, White breasted and White plumage pattern was performed and the plumage colour of progeny in different crosses was recorded. The results indicated dominant nature of White breasted and the recessive nature of White feather colour mutations. The inheritance of plumage colours of Pharao, White breasted and White could be explained on the basis of two autosomal plumage color loci

Chitosan nanoparticles: a versatile platform for biomedical applications

Chitosan is a biodegradable and biocompatible natural polymer that has been extensively explored in recent decades. The Food and Drug Administration has approved chitosan for wound treatment and nutritional use. Furthermore, chitosan has paved the way for advancements in different biomedical applications including as a nanocarrier and tissue-engineering scaffold. Its antibacterial, antioxidant, and haemostatic properties make it an excellent option for wound dressings. Because of its hydrophilic nature, chitosan is an ideal starting material for biocompatible and biodegradable hydrogels. To suit specific application demands, chitosan can be combined with fillers, such as hydroxyapatite, to modify the mechanical characteristics of pH-sensitive hydrogels. Furthermore, the cationic characteristics of chitosan have made it a popular choice for gene delivery and cancer therapy. Thus, the use of chitosan nanoparticles in developing novel drug delivery systems has received special attention. This review aims to provide an overview of chitosan-based nanoparticles, focusing on their versatile properties and different applications in biomedical sciences and engineering.info:eu-repo/semantics/publishedVersio

The impact of pumpkin seed-derived silver nanoparticles on corrosion and cytotoxicity: a molecular docking study of the simulated AgNPs

ABSTRACTGreen-synthesized nanoparticles from pumpkin (Cucurbita pepo L.) seed extracts are economical and eco-friendly. Silver nanoparticles (AgNPs) and their selective cytotoxicity towards HCT116 and African Green Monkey Kidney, Vero cells were investigated. Chemical fingerprinting, heat stability, and 2D-images of nanoparticle size and morphology were determined using Fourier-transform infrared spectroscopy (FTIR), Thermogravimetric Analysis (TGA), Transmission Electron Microscopy (TEM), and Scanning electron microscopy-energy dispersive X-ray (SEM-EDX) on AgNPs. UV-vis examination shows surface plasmons in the wide peak at 417 nm, indicating polydisperse nanoparticles. Small silver nanoparticles (AgNPs) below 2 µm demonstrated a rod-like form and a tendency to agglomerate. SEM-EDX element analysis and fingerprinting confirmed the material as AgNPs. TEM indicated that the nanoparticles were generally spherical or ellipsoidal, equally dispersed, and averaged 26.08 nm in diameter with negligible aggregation. The AgNPs are also stable at a temperature of 220°C, indicating that the green material is quite robust at 150°C to 400°C. According to cytotoxic studies, AgNPs are toxic to cancer cells (HCT 116 cells), however they have no effect on Vero cells. AgNPs and tubulin (TUB) domain active sites have a significant affinity, according to molecular docking analysis. In an electrochemical investigation, biogenic AgNPs effectively prevented mild steel from corroding in a 1.0 M HCl solution

Design, Synthesis, and Biological Evaluation of Benzimidazole-Derived Biocompatible Copper(II) and Zinc(II) Complexes as Anticancer Chemotherapeutics

Herein, we have synthesized and characterized a new benzimidazole-derived “BnI” ligand and its copper(II) complex, [Cu(BnI)2], 1, and zinc(II) complex, [Zn(BnI)2], 2, using elemental analysis and various spectroscopic techniques. Interaction of complexes 1 and 2 with the biomolecules viz. HSA (human serum albumin) and DNA were studied using absorption titration, fluorescence techniques, and in silico molecular docking studies. The results exhibited the significant binding propensity of both complexes 1 and 2, but complex 1 showed more avid binding to HSA and DNA. Also, the nuclease activity of 1 and 2 was analyzed for pBR322 DNA, and the results obtained confirmed the potential of the complexes to cleave DNA. Moreover, the mechanistic pathway was studied in the presence of various radical scavengers, which revealed that ROS (reactive oxygen species) are responsible for the nuclease activity in complex 1, whereas in complex 2, the possibility of hydrolytic cleavage also exists. Furthermore, the cytotoxicity of the ligand and complexes 1 and 2 were studied on a panel of five different human cancer cells, namely: HepG2, SK-MEL-1, HT018, HeLa, and MDA-MB 231, and compared with the standard drug, cisplatin. The results are quite promising against MDA-MB 231 (breast cancer cell line of 1), with an IC50 value that is nearly the same as the standard drug. Apoptosis was induced by complex 1 on MDA-MB 231 cells predominantly as studied by flow cytometry (FACS). The adhesion and migration of cancer cells were also examined upon treatment of complexes 1 and 2. Furthermore, the in vivo chronic toxicity profile of complexes 1 and 2 was also studied on all of the major organs of the mice, and found them to be less toxic. Thus, the results warrant further investigations of complex 1

Evaluation of Transition Metal Complexes of Benzimidazole-Derived Scaffold as Promising Anticancer Chemotherapeutics

Three new transition metal complexes, Cu(II) 1, Co(II) 2, and Zn(II) 3 with ligand “bimnap” derived from 1-methyl-2-aminobenzimidazole and 2-hydroxynapthaldehyde were synthesized and characterized. The structure of the ligand was determined by single X-ray crystallography. All the three complexes, 1–3, were examined for the mode of interaction with biomolecule viz., calf thymus-DNA (CT-DNA) using various spectroscopic methods. The nuclease activity was performed against pBR322 DNA that exhibited concentration-dependent degradation of the nucleic acid. The mechanism of DNA cleavage was studied by the electrophoretic pattern in the presence of the radical scavengers. Also, the complexes 1–3 were analyzed for groove binding affinity. Moreover, in vitro cytotoxicities of the complexes 1–3 were tested against the five human cancer cell lines, i.e., HeLa, SK-MEL-1, HepG2, HT108, and MDA-MB 231. Also, the cell adhesion and migration properties upon treatment of cell lines with complexes 1–3, and consequently, their cell death pathway via apoptosis and necrosis were analyzed. Further, complexes 1–3 were studied in vivo for their toxicities and tolerabilities in mice. In sum, the complexes 1–3 showed merits of an effective anticancer agent in cell lines–based study while minor side effects were observed in vivo

Feasibility and Product Maturity of Compost Developed from Poultry Waste in Temperate Agroclimate of Kashmir Region

Present work was carried out in the Department of LPM, FVSc and AH (SKUAST- Kashmir) to assess the feasibility and compost maturity in terms of temperature and bio-mineral (Nitrogen, Phosphorus and Potassium) changes due to composting of poultry waste under the temperate agro-climatic conditions of Kashmir Valley. Poultry waste in the form of poultry carcass (including feathers) and litter manure was used for this study. Four treatment groups with four replicates each were formulated as: G1: Poultry carcass; litter manure, G2: Poultry carcass; litter manure; Paddy straw, G3: Poultry carcass; litter manure; effective microbes (Lactobacillus plantarum, Lactobacillus casei, Saccharomyces cerevisiae and Rhodopseudomonas palustris) and G4: Poultry carcass; litter manure; Paddy straw; effective microbes. During the primary stage of composting the group G4 had attained a significantly (P<0.05) highest peak temperature (0C) of 59.0±5.04 and 59.50±5.04 respectively during winter and summer seasons. Significantly (P<0.05) highest nitrogen content of 24.7 g/Kg was observed in group G4 at the end of secondary stage during summer season. The phosphorus content in end product of composting was significantly (P<0.05) highest (1.0 g/Kg) in group G4 during summer season. Similarly significantly (P<0.05) highest K content of 10.23 g/Kg was observed in group G4 during summer season. It was concluded that the addition of paddy straw and effective microbes assistant the composting process and yield better bio-mineral values and higher temperature gain

One-Pot Synthesis of Benzopyrano-Pyrimidine Derivatives Catalyzed by P-Toluene Sulphonic Acid and Their Nematicidal and Molecular Docking Study

A cost-effective and environmentally benign benzopyrano-pyrimidine derivative synthesis has been established with the condensation of different salicylaldehyde derivatives, piperidine/morpholine with malononitrile, in the presence of a catalyst containing p-toluene sulphonic acid (PTSA) at 80 °C temperature. This procedure offers a new and enriched approach for synthesizing benzopyrano-pyrimidine derivatives with high yields, a straightforward experimental method, and short reaction times. The synthesized compounds were investigated for their nematocidal activity, and the result shows that among the four compounds, compounds 4 and 5 showed strong nematocidal activity against egg hatching and J2s mortality. The nematocidal efficacy of the compounds might be due to the toxicity of chemicals which are soluble in ethanol. The nematocidal effectiveness was directly related to the concentration of ethanolic dilutions of the compounds, i.e., the maximum treatment concentration, the higher the nematocidal action, or the higher the mortality and egg hatching inhibition. In the present study, with support from docking analysis, the relation between chemical reactivity and nematocidal activity of compound 4 was inferred

One-Pot Synthesis of Benzopyrano-Pyrimidine Derivatives Catalyzed by P-Toluene Sulphonic Acid and Their Nematicidal and Molecular Docking Study

A cost-effective and environmentally benign benzopyrano-pyrimidine derivative synthesis has been established with the condensation of different salicylaldehyde derivatives, piperidine/morpholine with malononitrile, in the presence of a catalyst containing p-toluene sulphonic acid (PTSA) at 80 °C temperature. This procedure offers a new and enriched approach for synthesizing benzopyrano-pyrimidine derivatives with high yields, a straightforward experimental method, and short reaction times. The synthesized compounds were investigated for their nematocidal activity, and the result shows that among the four compounds, compounds 4 and 5 showed strong nematocidal activity against egg hatching and J2s mortality. The nematocidal efficacy of the compounds might be due to the toxicity of chemicals which are soluble in ethanol. The nematocidal effectiveness was directly related to the concentration of ethanolic dilutions of the compounds, i.e., the maximum treatment concentration, the higher the nematocidal action, or the higher the mortality and egg hatching inhibition. In the present study, with support from docking analysis, the relation between chemical reactivity and nematocidal activity of compound 4 was inferred

Comparative anticancer and antimicrobial activity of aerial parts of Acacia salicina, Acacia laeta, Acacia hamulosa and Acacia tortilis grown in Saudi Arabia

The standardized ethanol extract (EE) of aerial parts of four Acacia species [A. salicina (ASEE), A. laeta (ALEE), A. hamulosa (AHEE), and A. tortilis (ATEE)] were examined in order to compare their cytotoxic and antimicrobial activities. All the extracts were standardized by UPLC- PDA method using rutin as standard compound. The extracts ALEE, AHEE and ATEE were found to contain rutin along with several other phytoconstituents while rutin was absent in ASEE. All the extracts showed varying level of antimicrobial activity with zone of inhibition ranged from 11 to 21 mm against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Candida albicans. The ALEE and ATEE showed relatively high antimicrobial potency (MIC = 0.2 to 1.6 mg mL−1) in comparison to other extracts. All the extracts were found to reduce the biofilm of P. aeruginosa PAO1 strain significantly in comparison to the untreated control. The cytotoxic property of ASEE, ALEE, AHEE, ATEE were evaluated against HepG2 (Liver), HEK-293 (Kidney), MCF-7 (Breast) and MDA-MB 231 (Breast) cancer cells. Of these, ALEE, AHEE and ATEE exhibited moderate cytotoxic property against human liver carcinoma cells (HepG2; IC50 = 46.2, 39.2 and 42.3 μg mL−1, respectively) and breast cancer cell lines (MCF-7; IC50 = 57.2, 55.3 and 65.7 μg mL−1, respectively). The ATEE and ALEE showed moderate cytotoxicity against HEK-293 (kidney) cells with IC50 = 49.1 and 53.5 μg mL−1, respectively. Since, Acacia species (A. laeta and A. hamulosa) contains numerous polyphenols which might prove to be highly cytotoxic and antimicrobial agents, we suggest that these species can be further subjected to the isolation of more cytotoxic and antimicrobial compounds. Keywords: Acacia, Antimicrobial, Cytotoxicity, Rutin, Standardizatio