Variables affecting the combustion efficiency of a clinical waste incineration process

Pembakaran merupakan satu kaedah pelupusan dianggap terbaik bagi pembuangan sisa klinikal terutamanya sisa buangan patologi. Operasi yang cekap bagi sesuatu pembakar itu penting bagi memastikan keberkesanan pemusnahan sisa buangan tersebut. Pembolehubah seperti kadar kemasukan sisa (sisa), kedua-dua suhu pembakaran bagi kebuk pembakaran primer (PCC) dan kebuk pembakaran sekunder (SCC), bahan api dan udara bagi pembakaran memainkan peranan penting dalam mempengaruhi prestasi proses pembakaran. Kecekapan pembakaran iaitu salah satu petunjuk prestasi proses pembakaran yang ditentukan oleh kewujudan emisi karbon monoksida dan karbon dioksida selepas SCC perlulah sentiasa diawasi. Justeru itu, satu kajian meninjau hubungan di antara pembolehubah yang mempengaruhi kecekapan pembakaran seperti sisa, suhu dan bahan api telah dijalankan menggunakan analisis regresi yang ditentusahkan menggunakan ANOVA. Keputusan kajian menunjukkan bahawa sisa, suhu pembakaran bagi kebuk pembakaran primer dan kebuk pembakaran sekunder menyumbang kepada kecekapan pembakaran bagi proses ini. Korelasi di antara pembolehubah ini juga dikaji dan dibincangkan

Evolving accelerated amidation by SpyTag/SpyCatcher to analyze membrane dynamics

SpyTag is a peptide that forms a spontaneous amide bond with its protein partner SpyCatcher. This protein superglue is a broadly useful tool for molecular assembly, locking together biological building blocks efficiently and irreversibly in diverse architectures. We initially developed SpyTag and SpyCatcher by rational design, through splitting a domain from a Gram-positive bacterial adhesin. Here we establish a phage display platform able to select for specific amidation, achieving an order of magnitude acceleration for interaction of the SpyTag002 variant with the SpyCatcher002 variant. We show that the 002 pair bonds rapidly under a wide range of conditions and at either protein terminus. SpyCatcher002 was fused to an intimin derived from enterohemorrhagic Escherichia coli. SpyTag002 reaction enabled specific and covalent decoration of intimin, for live cell fluorescent imaging of the dynamics of the bacterial outer membrane as cells divide

Evolving accelerated amidation by SpyTag/SpyCatcher to analyze membrane dynamics

SpyTag is a peptide that forms a spontaneous amide bond with its protein partner SpyCatcher. This protein superglue is a broadly useful tool for molecular assembly, locking together biological building blocks efficiently and irreversibly in diverse architectures. We initially developed SpyTag and SpyCatcher by rational design, through splitting a domain from a Gram-positive bacterial adhesin. Here we establish a phage display platform able to select for specific amidation, achieving an order of magnitude acceleration for interaction of the SpyTag002 variant with the SpyCatcher002 variant. We show that the 002 pair bonds rapidly under a wide range of conditions and at either protein terminus. SpyCatcher002 was fused to an intimin derived from enterohemorrhagic Escherichia coli. SpyTag002 reaction enabled specific and covalent decoration of intimin, for live cell fluorescent imaging of the dynamics of the bacterial outer membrane as cells divide

SpySwitch enables pH- or heat-responsive capture and release for plug-and-display nanoassembly

Proteins can be empowered via SpyTag for anchoring and nanoassembly, through covalent bonding to SpyCatcher partners. Here we generate a switchable version of SpyCatcher, allowing gentle purification of SpyTagged proteins. We introduce numerous histidines adjacent to SpyTag’s binding site, giving moderate pH-dependent release. After phage-based selection, our final SpySwitch allows purification of SpyTag- and SpyTag003-fusions from bacterial or mammalian culture by capture at neutral pH and release at pH 5, with purity far beyond His-tag methods. SpySwitch is also thermosensitive, capturing at 4 °C and releasing at 37 °C. With flexible choice of eluent, SpySwitch-purified proteins can directly assemble onto multimeric scaffolds. 60-mer multimerization enhances immunogenicity and we use SpySwitch to purify receptor-binding domains from SARS-CoV-2 and 11 other sarbecoviruses. For these receptor-binding domains we determine thermal resilience (for mosaic vaccine development) and cross-recognition by antibodies. Antibody EY6A reacts across all tested sarbecoviruses, towards potential application against new coronavirus pandemic threats.publishedVersionPeer reviewe

Capitol Update piece reporting that Mainers still pay to store nuclear waste f

Capitol Update piece reporting that Mainers still pay to store nuclear waste from the Maine Yankee facility, more than a decade after it stopped generating power. Richard Davies, the state public advocate, says the average Mainer pays about 50 cents each month to store the waste. As of the end of 2007, Mainers had paid over $189 million to dispose of nuclear waste generated by the decommissioned Maine Yankee, as well as waste generated by plants like Vermont Yankee that supplied power to Maine. Since Maine Yankee\u27s closure in 1997, none of the waste has been stored in a permanent waste facility as required by federal law. The federal nuclear waste depository at Yucca Mountain in Nevada is now estimated to be complete no sooner than 2020, and the process of shipping all of Maine\u27s waste there is estimated to be completed at 2037 at the earliest