Combination index for IFN-α and IFN-λ1 against CCHF replication respectively in A549 and HuH-7 cells.

<p>*The Combination Index (CI) was calculated using the CompuSyn software (Chou, T.-C. and Martin, N. CompuSyn software for drug combinations and for general dose effect analysis, and user’s guide. ComboSyn, Inc. Paramus, NJ 2007. [<a href="http://www.combosyn.com" target="_blank">www.combosyn.com</a>]) which uses the method of Chou & Talalay. CI values <1, 1 and >1 indicate synergism, additive effect and antagonism, respectively.</p><p>Combination index for IFN-α and IFN-λ1 against CCHF replication respectively in A549 and HuH-7 cells.</p

Dose-dependent inhibition of CCHFV replication by recombinant IFN-α, IFN-λ1 and IFN-α+IFN-λ1.

<p>A549 <b>(a)</b> and HuH7 cells <b>(b)</b> were treated for 1 day with increasing amounts of either IFN type, alone or in combination, then infected with CCHFV at MOI 0.01; infectious virus yield was measured after overnight incubation. One out of three experiments is shown. Dotted lines: IFN-α (●) or IFN-λ1 (▲) used alone; continuous line: IFN-α and IFN-λ1 (■) used in combination.</p