Farklı fonksiyonel gruplara sahip kaliksaren temelli polikarilonitril nanofiberlerin sentezi ve protein adsorpsiyon çalışmaları

TEZ 540/KESfKaynakça: 110-120 ss.[Özet Yok

Novel integrated sensing system of calixarene and rhodamine molecules for selective colorimetric and fluorometric detection of Hg2+ ions in living cells

WOS:000587559700023 PubMed ID: 32932034Three novel and facile calixarene derivatives (5, 6 and 7), which were appended with four rhodamine units at the upper rim of calixarene skeleton, were firstly prepared and evaluated for selective detection of metal ions in solution. Receptors (5) and(7) indicated immediate turn on fluorescence output toward Hg2+ ions over other most competitive metal ions with the ultralow detection limits, indicating their high efficiency and reliability. The binding response to Hg2+ ions in solution was also observed through a chromogenic change (from colorless to pale pink). Furthermore, in vitro and bio-imaging studies with MCF-7 or MIA PaCa-2 cell lines were also performed to investigate the use of receptors in biological systems in order to monitor of mercury ions. Results showed that new receptors (5) or (7) were cell permeable and suitable for real-time imaging of Hg2+ in living cells (MCF-7) or (MIA PaCa-2) without any damage to healthy cell lines (HEK 293)

Antibacterial, thermal decomposition and in vitro time release studies of chloramphenicol from novel PLA and PVA nanofiber mats

WOS:000624405100004PubMed ID: 33641898This study is supported by financial support from the Scientific and Technological Research Council of Turkey (TUBITAK grant no: 118Z511)The present investigation is the first report containing design and synthesis of novel calixarene derivatives (6–8) and their inclusion complexes (IC6–IC8) with Chloramphenicol (CAM). After synthesis, the antibiotic CAM, calixarene derivatives (6–8) and their inclusion complexes (IC6–IC8) were successfully incorporated into biodegradable PVA and/or PLA nanofiber skeleton by electrospinning. The obtained electrospun nanofibers were tested and compared for inhibition of bacterial growth towards multiple bacterial species (Escherichia coli, and Bacillus subtilis). Moreover, we evaluated thermal decomposition and release profile of CAM by spectrophotometric methods. The results suggested that CAM can be successfully encapsulated in nanofiber webs by inclusion complexation, and these fibers could be used as a part of new controlled release packaging system for food preservation

A highly selective optical sensor for the detection of cyanide ions in aqueous solution and living cells

WOS:000730122000001A fluorogenic naphthalene based receptor (NFS) has been successfully generated by preparation from 3-amino-2-naphthol and 2-hydroxy-1-naphthaldehyde, it was then characterized. Optical and colorimetric properties of the receptor (NFS) in terms of cyanide (CN-) ions were investigated by several methods including naked-eye detection, UV–vis spectrophotometry and fluorescence spectroscopy. A visual color change from yellow to orangish-pink was observed upon interaction of the receptor with cyanide (CN-) ions in solution, while no color change appeared upon addition of the other anions tested. The detection limit of NFS was calculated as 0.21 µM without any interaction with other anions tested which is lower than that described by the World Health Organization for CN– ions in drinking water. It can be concluded that the receptor (NFS) was sensitive and rapid enough for the detection of CN- ions in solutions. The interaction between the receptor and CN– ions was also investigated by 1H NMR titration, MALDI-Tof mass spectrometry, and FTIR-AR spectroscopy. NFS was also applied to the bio-imaging of human epithelial cell line (HEK 293) and the cell imaging studies demonstrated that the receptor was capable of detecting cyanide ions in living cells

Electrospinning of novel calixarene-functionalized PAN and PMMA nanofibers: Comparison of fluorescent protein adsorption performance

WOS:000424174900060This present paper is the first report showing the preparation and protein binding properties of new polyacrylonitrile (PAN) and/or Poly(methyl methacrylate) (PMMA) nanofibers based calixarene tetraester units by electrospinning of calixarene tetraester and PAN or PMMA solutions in DMF. The newly prepared nanofibers materials was fully characterized by using spectroscopic techniques. From the obtained spectroscopic results, existing of calixarene molecules onto the fiber matrix was proved by the presence of the characteristic calixarene bands in FTIR spectra and considerable changing of surface morphologies of fiber matrix in SEM image. Furthermore, the protein extraction studies were performed by batch method toward green fluorescence protein (GFP) as a model protein and protein binding results exhibited that nanofibers based calixarene could be actively used as a support material for the binding of green fluorescence protein (GFP) from aqueous solutions. The results were compared with bare PAN and PMMA without calixarene and it was observed that GFP binding was higher in the case of calixarene-coated nanofibers especially for nanofibers containing calixarene ester derivatives. In addition, amino acid extraction studies were also performed to clarify the possible binding interaction between nano fibers and protein. From the amino acid extraction experiments, it was determined that the excellent interaction between protein and nanofibers became with amino acids having aromatic side arms such as phenylalanine and tryptophan. (C) 2017 Elsevier B.V. All rights reserved.Karamanoglu Mehmetbey University Research FoundationKaramanoglu Mehmetbey University [25-M-15]The authors declare that there is no conflict of interest. This study is part of master thesis of Mukaddes Keskinates and authors of this paper gratefully would like to thank Karamanoglu Mehmetbey University Research Foundation (Project number 25-M-15) for financial support

An isonicotinohydrazide based fluorescence sensor for detection of Zn2+ in biological systems: Experimental and theoretical studies along with cell imagine

Zinc ions (Zn2+) play a significant role in our daily lives and production, which is related to human health and environment protection. Therefore, it is crucial in this field to develop sensors with improved analyte selectivity and sensitivity. We prepared a fluorescent sensor, DIH, through the reaction of 5-(tert-butyl)-2-hydroxyisophthalaldehyde with isonicotinohydrazide. DIH, in free form, can undergo excited state intramolecular proton transfer (ESIPT) process upon excitation. Among a wide range of metal ions tested, only Zn2+ inhibited this ESIPT process, resulting in an increase in fluorescence intensity (13.3-fold) with chelation enhancement fluorescence process (CHEF). The complex (DIH/Zn2+) stoichiometry was determined to be 1:1 and the binding constant of the complex was estimated as 3.18 × 107 M−1. DIH could also be applied to detect zinc ions down to a low concentration level of 11.2 nanomolar with fluorescence spectroscopy. Moreover, the cytotoxicity evaluation of DIH against human colon adenocarcinoma cells lines demonstrated that the sensor was nontoxic and could be used successfully in cellular imagin

A novel fluorescent sensor based on an enzyme-free system for highly selective and sensitive detection of glyphosate and malathion in real samples

Malathion (Mal) and glyphosate (Glyp) are widely used organophosphorus pesticides to prevent or control insects and weed in agriculture. Unfortunately, the extensive and frequent commercial use of these compounds on farmland has caused their presence as residues in crops and livestock. Therefore, the design of new sensors with enhanced analyte selectivity and sensitivity is critical in this field. Here, we developed an optical sensor for the detection of malathion and glyphosate. The sensor, N,N-dimethyl-4-(3-(pyrazin-2-ylimino)prop-1-en-1-yl)aniline (CNP), was synthesized in a one-step reaction by condensation of 4-(dimethylamino)cinnamaldehyde with 2-aminopyrazine. CNP gives a rapid color change, absorbance and fluorescence response to Glyp and Mal in the presence of the metal ions and pesticides tested in the working system. The binding stoichiometry between the sensor and the pesticides (Glyp and Mal) was investigated by some spectrophotometric methods and the sensing mechanisms were eludicated by NMR titration experiments, suggesting that CNP responds to Glyp via H-bonding and to Mal via the coordination. The sensor has a high selectivity and rapid response to the pesticides (Glyp and Mal) with low detection limits. The sensor was also successfully used to monitor the pesticides in real samples; Mal in apple and tap water samples, and Glyp in potato, tap water and soil samples

A rapid responsive coumarin-naphthalene derivative for the detection of cyanide ions in cell culture

WOS:000841161600003PubMed ID:35779572Cyanide ion (CN−) is widely used in many industrial processes; however, it causes several diseases in humans. Therefore, rapid and accurate detection of CN− is very important and urgent. In this study, a CN− sensor (MH-2) which was capable of detecting CN− ions in living cell was developed. MH-2 gives a rapid color change, absorbance and fluorescence response to CN− in the presence of the anions tested in the working system. The binding ratio between the sensor and CN− was demonstrated by some spectrophotometric methods and the sensing mechanism was investigated by NMR titration experiments, suggesting that MH-2 gives response to CN− via the nucleophilic addition reaction. The fluorescence detection limit and the absorbance detection limit were calculated as 0.056 μM and 0.11 μM, respectively. Both of these detection limits are below the tolerable limit recommended by WHO for CN− in the drinking water (1.9 μM). MH-2 was also applied to living cells for bio-imaging and the results showed that the sensor penetrates the cells and can detect cyanide ions in living cells

Two novel enzyme-free colorimetric sensors for the detection of glyphosate in real samples

The intensive use and accumulation of glyphosate cause many environmental and ecological problems. There is an urgent need for an effective detection method for glyphosate. Many studies in the literature determine glyphosate by an enzyme-based method, requiring appropriate temperature, pH, substrate and ionic strength. In this study, we presented a simple enzyme-free reaction system. Two new simple Schiff base derivatives, 4-(((3-chloropyridin-4-yl)imino)methyl)-N,N-dimethylaniline (BNP-Cl) and 4-((3-((3-chloropyridin-4-yl)imino)propenyl)-N,N-dimethylaniline (CNP-Cl), were prepared by combining a pyridine moiety with a cinnamaldehyde or a benzaldehyde moiety. The pyridine moiety contains a chlorine atom, and this chlorine atom is replaced by the nitrogen or oxygen of glyphosate (Glyp) in ACN/Tris buffer (pH=8.14, 10 mM, v/v 2:1). This binding caused color changes ranging from colorless to yellow for BNP-Cl and yellow to orange for CNP-Cl. The sensors BNP-Cl and CNP-Cl have a rapid and excellent selectivity for glyphosate with low detection limits (1.78 µM for BNP-Cl and 1.60 µM for CNP-Cl). The sensors were also successfully applied to detect Glyp in real samples such as soil, tap water, and potatoes