A Strategy for Dual Biopolymer Production of P(3HB) and γ-PGA

BACKGROUND Production of biopolymers has gained considerable attention because of their biodegradability, biocompatibility, and as suitable replacements for mineral-based polymers. Despite advances in production process, a notable drawback still exists due to high production cost. The aim of this paper is to provide a production strategy for cost reduction. The suggested process may be adopted to other polymers, useable to wide audience in biopolymer research. RESULTS Dual production of two commercially important biopolymers, P(3HB) and γ-PGA, in a single batch from cheap substrates was studied, as proof of concept, for a feasible low cost dual biopolymer production. The dual production from a single batch yielded 1 g/L P(3HB) and 0.4 g/L γ-PGA using Bacillus subtilis OK2 (B. subtilis OK2). When orange peel was substituted as a cheap carbon source for dual production, coupled pH and dissolved oxygen control proved to be essential to overcome the inhibition imposed by the non-sugar components of the substrate. The cell lysis and release of P(3HB) granules in the dual production medium can be exploited of as a new approach for separation of this polymer. CONCLUSION This proof of concept study provides a new approach from upstream to downstream processing for low cost production of dual biopolymers

Predicting individual susceptibility to Visually Induced Motion Sickness (VIMS) by Questionnaire

BACKGROUND The introduction of new visual technologies increases the risk of visually induced motion sickness (VIMS). The aim was to evaluate the 6-item Visually Induced Motion Sickness Susceptibility Questionnaire (VIMSSQ; also known as the VIMSSQ-short) and other predictors for individual susceptibility to VIMS. METHODS Healthy participants (10M+20F), mean age 22.9 (SD 5.0) years, viewed a 360° panoramic city scene projected in the visual equivalent to the situation of rotating about an axis tilted from the vertical. The scene rotated at 0.2Hz (72° s-1), with a ‘wobble’ produced by superimposed 18° tilt on the rotational axis, with a field of view of 83.5°. Exposure was 10 min or until moderate nausea was reported. Simulator Sickness Questionnaire (SSQ) was the index of VIMS. Predictors/correlates were VIMSSQ, Motion Sickness Susceptibility Questionnaire (MSSQ), Migraine (scale), Syncope, Social & Work Impact of Dizziness (SWID), Sleep quality/disturbance, Personality (‘Big Five’ TIPI), a prior multisensory Stepping-Vection test, and Vection during exposure. RESULTS The VIMSSQ had good scale reliability (Cronbach’s alpha=0.84). and correlated significantly with the SSQ (r=0.58). Higher MSSQ, Migraine, Syncope & SWID also correlated significantly with SSQ. Other variables had no significant relationships with SSQ. Regression models showed that the VIMSSQ predicted 34% of the individual variation of VIMS, increasing to 56% as MSSQ, Migraine, Syncope and SWID were incorporated as additional predictors. CONCLUSIONS The VIMSSQ is a useful adjunct to the MSSQ in predicting VIMS. Other predictors included Migraine, Syncope & SWID. No significant relationship was observed between Vection and VIMS

Agro-Industrial Waste Materials as Substrates for the Production of Poly(3-Hydroxybutyric Acid)

Accumulation of recalcitrant plastics in the environment has become a world-wide problem in today’s societies. Rapid depletion of natural resources for synthetic plastics along with environmental concerns has directed research towards finding alternatives to petroleum-based polymers. Poly(3-hydroxybutyric acid) P(3HB), as one of these alternatives, have attracted much attention in recent years due to their varied mechanical properties, biocompatibility and iodegradability. The aim of this study was to identify an agro-industrial waste resource economically suitable for large-scale production of P(3HB), to optimize the production using Response Surface Methodology in small-scale and subsequently, to test the production in a continuously stirred tank reactor. Among a range of agro-industrial waste, orange peel was selected as the most suitable for P(3HB) production. P(3HB) concentration of 1.24 g P(3HB)/L culture broth with 41% P(3HB)/dcw yield was obtained using orange peel as the sole carbon source in optimized medium with a modified strain of Bacillus subtilis (B. subtilis OK2)

Numerical Modeling of Sediment Transport in a Typical Large Reservoir with Respect to Meander Effects on Sediment Flow Path

Source: ICHE Conference Archive - https://mdi-de.baw.de/icheArchiv

Compact active duplexer based on CSRR and interdigital loaded microstrip coupled lines for LTE application

In this paper, a four-port compact active duplexer based on a complimentary split ring resonator (CSRR) and interdigital loaded microstrip coupled lines (CSRR-IL MCL) is presented. Interdigital capacitor is used on the top layer of the proposed structure and CSRR transmission lines are used on the bottom layer of the coupled lines in order to increase the coupling of the proposed circuit and create triple band resonances, respectively. The proposed active duplexer has one input port and three output ports operating in three distinct operation frequencies which are 1.4 GHz, 1.8 GHz, and 3.2 GHz. The active duplexer is designed to target LTE applications which are prevalent among the new technologies and devices. The input signal is split in terms of frequency into the three designed frequencies and is amplified by 13 dB gain of the amplifiers placed at the output ports. The fractional bandwidths of the proposed structure at 1.4 GHz, 1.8 GHz, and 3.2 GHz are 5.2%, 2.8%, and 9.4%, respectively. It is worth mentioning that the size of the proposed active duplexer is 0.29λ0 × 0.38λ0. The design guide of the proposed structure is presented, and it will be shown that the simulation as well as the measurement results of the proposed active duplexer have an acceptable agreement with each other. It should be noted that the VSWR of the proposed structure is less than 1.5 which means that the active duplexer has low return loss, and it is the plus point of it

Influence of Pichia pastoris cellular material on polymerase chain reaction performance as a synthetic biology standard for genome monitoring

Advances in synthetic genomics are now well underway in yeasts due to the low cost of synthetic DNA. These new capabilities also bring greater need for quantitating the presence, loss and rearrangement of loci within synthetic yeast genomes. Methods for achieving this will ideally; i) be robust to industrial settings, ii) adhere to a global standard and iii) be sufficiently rapid to enable at-line monitoring during cell growth. The methylotrophic yeast Pichia pastoris (P. pastoris) is increasingly used for industrial production of biotherapeutic proteins so we sought to answer the following questions for this particular yeast species. Is time-consuming DNA purification necessary to obtain accurate end-point polymerase chain reaction (e-pPCR) and quantitative PCR (qPCR) data? Can the novel linear regression of efficiency qPCR method (LRE qPCR), which has properties desirable in a synthetic biology standard, match the accuracy of conventional qPCR? Does cell cultivation scale influence PCR performance? To answer these questions we performed e-pPCR and qPCR in the presence and absence of cellular material disrupted by a mild 30s sonication procedure. The e-pPCR limit of detection (LOD) for a genomic target locus was 50 pg (4.91 × 103 copies) of purified genomic DNA (gDNA) but the presence of cellular material reduced this sensitivity sixfold to 300 pg gDNA (2.95 × 104 copies). LRE qPCR matched the accuracy of a conventional standard curve qPCR method. The presence of material from bioreactor cultivation of up to OD600 = 80 did not significantly compromise the accuracy of LRE qPCR. We conclude that a simple and rapid cell disruption step is sufficient to render P. pastoris samples of up to OD600 = 80 amenable to analysis using LRE qPCR which we propose as a synthetic biology standard

Layer-resolved magnetic exchange interactions of surfaces of late 3d elements: effects of electronic correlations

We present the results of an ab initio study of magnetic properties of Fe, Co and Ni surfaces. In particular, we discuss their electronic structure and magnetic exchange interactions (Jij), as obtained by means of a combination of density functional theory and dynamical mean-field theory. All studied systems have a pronounced tendency to ferromagnetism both for bulk and surface atoms. The presence of narrow-band surface states is shown to enhance the magnetic moment as well as the exchange couplings. The most interesting results were obtained for the Fe surface where the atoms have a tendency to couple antiferromagnetically with each other. This interaction is relatively small, when compared to interlayer ferromagnetic interaction, and strongly depends on the lattice parameter. Local correlation effects are shown to lead to strong changes of the overall shape of the spectral functions. However, they seem to not play a decisive role on the overall picture of the magnetic couplings studied here. We have also investigated the influence of correlations on the spin and orbital moments of the bulk-like and surface atoms. We found that dynamical correlations in general lead to enhanced values of the orbital moment.Comment: 13 pages, 12 figure

Transcriptomic Complexity of Aspergillus terreus Velvet Gene Family under the Influence of Butyrolactone I

Filamentous fungi of the Ascomycota phylum are known to contain a family of conserved conidiation regulating proteins with distinctive velvet domains. In Aspergilli, this velvet family includes four proteins, VeA, VelB, VelC and VosA, and is involved in conidiation and secondary metabolism along with a global regulator LaeA. In A. terreus, the overexpression of LaeA has been observed to increase the biogenesis of the harmaceutically-important secondary metabolite, lovastatin, while the role of the velvet family has not been studied. The secondary metabolism and conidiation of A. terreus have also been observed to be increased by butyrolactone I in a quorum-sensing manner. An enlightenment of the interplay of these regulators will give potential advancement to the industrial use of this fungus, as well as in resolving the pathogenic features. In this study, the Aspergillus terreus MUCL 38669 transcriptome was strand-specifically sequenced to enable an in-depth gene expression analysis to further investigate the transcriptional role of butyrolactone I in these processes. The sequenced transcriptome revealed intriguing properties of the velvet family transcripts, including the regulator laeA, and uncovered the velC gene in A. terreus. The reliability refining microarray gene expression analysis disclosed a positive regulatory role for butyrolactone I in laeA expression, as well as an influence on the expression of the canonical conidiation-regulating genes under submerged culture. All of this supports the suggested regulative role of butyrolactone I in A. terreus secondary metabolism, as well as conidiation