Enhancing Fruit Yield in 'Ney Poovan' Banana (Musa paradisiaca L.) by De-Navelling and Feeding N, K and S through Distal Stalk-End of the Bunch

De-navelling and feeding ammonium sulphate (AS) (5-25 g/plant) with or without potassium sulphate (2.5-12.5 g/ plant) blended in 500 g of fresh cow-dung and applied to the distal stalk-end of the bunch of 'Ney Poovan' banana (Musa sp. L., AB) showed that the nutrients moved from the blend into the bunch and significantly enhanced weight of the fruits and of the bunch, compared to retention of flower, de-navelling (removal of male inflorescence) and application of 500 g cow-dung only to the excised distal stalk- end of the bunch. De-navelling caused 7.1% (5623 g) higher bunch yield, which increased to 13.9% (5980 g) when cow dung alone was applied after de-navelling. When cow dung was blended with 5 g of AS and 2.5 g of Sulphate of Potash, the response was 66.5% (9362 g) over de-navelling and application of cow dung alone and 78.3% (9362 g) over retention of male bud throughout (5250 g). A significantly higher N content, N uptake, Ndff (nitrogen derived from fertilizer), fertilizer N uptake, utilization of fertilizer and K and S content were observed when cow-dung enriched with AS and SOP was applied. Nitrogen content and all the parameters of N use were distinctly higher in the basal portion of the bunch indicating the flow of the applied nutrients upward from the de-navelled end. Results showed that application of 5 g ammonium sulphate and 2.5 g sulphate of potash blended in 500 g of fresh cow dung to the distal stalk-end of the bunch of 'Ney Poovan' banana was the most promising in boosting the yield, improving the nutritional composition in respect of N, K and S without adversely affecting the fruit quality

Influence of De-Navelling and Stalk-End Nutrient Application on Nutrient Composition of 'Robusta' Banana Fruits

The contents of N, P, Mg, S, Fe and Mn in banana fruit increased significantly due to denavelling from 0.32%, 0.086%, 0.12%, 0.024%, 52 ppm and 4.8 ppm, under 'control' to 0.37%, 0.085%, 0.13%, 0.027%, 59 ppm and 6.7 ppm, respectively. Dipping stalk end of the bunch in fresh cow dung enhanced these above nutrients to 0.40%, 0.086%, 0.14%. 0.028%, 63 ppm and 7.6 ppm, respectively. When cow dung was enriched with ammonium sulphate, the fruits showed 0.50-0.51% of N, 0.081-0.090% of P, 0.16-0.23% of Mg, 0.032-0.040% of S, 59-111 ppm of Fe and 8.1-17.8 ppm of Mn. Addition of potassium sulphate further enhanced this effect in respect of K (2.11-2.44%) and Fe (74-115 ppm) in fruit. Increasing level of ammonium sulphate in the blend significantly decreased Ca content of the fruit from 0.24% at 5 g to 0.10% at 25 g. When potassium sulphate was included in the blend, Ca content showed further reduction (0.19% at 5 g to 0.10% at 25g). At 15 g of ammonium sulphate and 7.5 g of potassium sulphate the maximum bunch weight of 27.993 kg was obtained (as against 16.724kg under retention of male bud throughout) corresponding to the enhanced nutrient composition of 2.44% of K, 0.12% of Ca, 0.18% of Mg, 0.033% of S, 115ppm of Fe and 14.9ppm of Mn that may have nutraceutical implications

Nutrient Dynamics of Annual Growth-Flush in Mango (Mangifera indica L.)

Internal nutrient dynamics in mango (cv. Alphonso) were studied during its annual growth flush (January - June, 2002). The study consisted of sampling mature leaves and growth belonging to the previous thirteen seasons at least (representing the seasonal growth of the previous six years) at fruit-set and post-harvest stages of plant growth. The samples were analyzed for N, P, K, Ca and Mg. The study indicated that phosphorus moved from 2nd, 3rd and 4th internodes to current season's growth and accumulated at other internodes, potassium moved from mature leaves to the new growth and accumulated in all the other internodes. Calcium and magnesium moved from 9th and older internodes to current season's growth, whereas, N was mostly remobilized from much older parts and by absorption from soil. The results imply that fertilizer application in productive mango trees should aim at keeping nutrient reserves of the permanent framework well-supplied to achieve sustained fruit production

An Investigation on the Influence of Modeling Approach and Load Pattern on Seismic Performance of RC Structures

Non-linear Static Analysis serves as a suitable measure to evaluate the performance of a structural system. The careful selection of modelling approach and the load pattern is critical to arrive at an adequate performance evaluation. The present study seeks to evaluate and compare the response of an existing eight story reinforced concrete structure, through the application of different modeling approaches and load patterns prescribed by FEMA 356. The results indicates that, with extreme clarity, that in all cases, the shape of the lateral load distribution is what the response of the buildings is finely accustomed to. This is especially true when different patterns of load are considered. It can also be observed that there is a very small difference between various load patterns

Volar plating of isolated ulna shaft fractures

Background: Dorsal plating for ulna shaft fracture is a common practice. But this is associated with hardware prominence on the dorsal subcutaneous border of ulna necessitating implant removal on later days. Volar surface of ulna is flat similar to radius volar surface with good muscle cover reducing the problem of hardware prominence. So, we wanted to study the outcome of volar plating of ulnar shaft fractures.  Methods: Ten patients satisfying our inclusion criteria underwent volar plating using volar approach between FCU and ECU and 3.5 DCP was placed on flat volar surface of ulnar shaft under thick muscle cover of FCU and FDP. Results: Out of 10 patients, 7 were acute fractures, 2 were neglected non unions and 1was non-union with implant insitu. Bone graft was used in non-union cases. All fractures united at 6-9 month post op without any complications. Conclusions: Isolated ulnar shaft fractures are common orthopaedic injuries. Displaced fractures require stabilization with dynamic compression plate (DCP). Application of implant on its volar aspect in distal 2/3rd fractures is easy due to flat surface and avoids complications related to hard ware prominence and subsequent need for implant removal

Key Scientific Issues in the Health Risk Assessment of Trichloroethylene

Trichloroethylene (TCE) is a common environmental contaminant at hazardous waste sites and in ambient and indoor air. Assessing the human health risks of TCE is challenging because of its inherently complex metabolism and toxicity and the widely varying perspectives on a number of critical scientific issues. Because of this complexity, the U.S. Environmental Protection Agency (EPA) drew upon scientific input and expertise from a wide range of groups and individuals in developing its 2001 draft health risk assessment of TCE. This scientific outreach, which was aimed at engaging a diversity of perspectives rather than developing consensus, culminated in 2000 with 16 state-of-the-science articles published together as an Environmental Health Perspectives supplement. Since that time, a substantial amount of new scientific research has been published that is relevant to assessing TCE health risks. Moreover, a number of difficult or controversial scientific issues remain unresolved and are the subject of a scientific consultation with the National Academy of Sciences coordinated by the White House Office of Science and Technology Policy and co-sponsored by a number of federal agencies, including the U.S. EPA. The articles included in this mini-monograph provide a scientific update on the most prominent of these issues: the pharmacokinetics of TCE and its metabolites, mode(s) of action and effects of TCE metabolites, the role of peroxisome proliferator–activated receptor in TCE toxicity, and TCE cancer epidemiology

Differential Gene Expression in Normal Human Mammary Epithelial Cells Treated with Malathion Monitored by DNA Microarrays

Organophosphate pesticides are a major source of occupational exposure in the United States. Moreover, malathion has been sprayed over major urban populations in an effort to control mosquitoes carrying West Nile virus. Previous research, reviewed by the U.S. Environmental Protection Agency, on the genotoxicity and carcinogenicity of malathion has been inconclusive, although malathion is a known endocrine disruptor. Here, interindividual variations and commonality of gene expression signatures have been studied in normal human mammary epithelial cells from four women undergoing reduction mammoplasty. The cell strains were obtained from the discarded tissues through the Cooperative Human Tissue Network (sponsors: National Cancer Institute and National Disease Research Interchange). Interindividual variation of gene expression patterns in response to malathion was observed in various clustering patterns for the four cell strains. Further clustering identified three genes with increased expression after treatment in all four cell strains. These genes were two aldo–keto reductases (AKR1C1 and AKR1C2) and an estrogen-responsive gene (EBBP). Decreased expression of six RNA species was seen at various time points in all cell strains analyzed: plasminogen activator (PLAT), centromere protein F (CPF), replication factor C (RFC3), thymidylate synthetase (TYMS), a putative mitotic checkpoint kinase (BUB1), and a gene of unknown function (GenBank accession no. AI859865). Expression changes in all these genes, detected by DNA microarrays, have been verified by real-time polymerase chain reaction. Differential changes in expression of these genes may yield biomarkers that provide insight into interindividual variation in malathion toxicity

Extracellular Vesicles in Triple–Negative Breast Cancer: Immune Regulation, Biomarkers, and Immunotherapeutic Potential

Triple–negative breast cancer (TNBC) is an aggressive subtype accounting for ~10–20% of all human BC and is characterized by the absence of estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor 2 (HER2) amplification. Owing to its unique molecular profile and limited targeted therapies, TNBC treatment poses significant challenges. Unlike other BC subtypes, TNBC lacks specific molecular targets, rendering endocrine therapies and HER2–targeted treatments ineffective. The chemotherapeutic regimen is the predominant systemic treatment modality for TNBC in current clinical practice. However, the efficacy of chemotherapy in TNBC is variable, with response rates varying between a wide range of patients, and the emerging resistance further adds to the difficulties. Furthermore, TNBC exhibits a higher mutational burden and is acknowledged as the most immunogenic of all BC subtypes. Consequently, the application of immune checkpoint inhibition has been investigated in TNBC, yielding promising outcomes. Recent evidence identified extracellular vesicles (EVs) as an important contributor in the context of TNBC immunotherapy. In view of the extraordinary ability of EVs to transfer bioactive molecules, such as proteins, lipids, DNA, mRNAs, and small miRNAs, between the cells, EVs are considered a promising diagnostic biomarker and novel drug delivery system among the prospects for immunotherapy. The present review provides an in–depth understanding of how EVs influence TNBC progression, its immune regulation, and their contribution as a predictive biomarker for TNBC. The final part of the review focuses on the recent key advances in immunotherapeutic strategies for better understanding the complex interplay between EVs and the immune system in TNBC and further developing EV–based targeted immunotherapies

Key Issues in the Modes of Action and Effects of Trichloroethylene Metabolites for Liver and Kidney Tumorigenesis

Trichloroethylene (TCE) exposure has been associated with increased risk of liver and kidney cancer in both laboratory animal and epidemiologic studies. The U.S. Environmental Protection Agency 2001 draft TCE risk assessment concluded that it is difficult to determine which TCE metabolites may be responsible for these effects, the key events involved in their modes of action (MOAs), and the relevance of these MOAs to humans. In this article, which is part of a mini-monograph on key issues in the health risk assessment of TCE, we present a review of recently published scientific literature examining the effects of TCE metabolites in the context of the preceding questions. Studies of the TCE metabolites dichloroacetic acid (DCA), trichloroacetic acid (TCA), and chloral hydrate suggest that both DCA and TCA are involved in TCE-induced liver tumorigenesis and that many DCA effects are consistent with conditions that increase the risk of liver cancer in humans. Studies of S-(1,2-dichlorovinyl)-l-cysteine have revealed a number of different possible cell signaling effects that may be related to kidney tumorigenesis at lower concentrations than those leading to cytotoxicity. Recent studies of trichloroethanol exploring an alternative hypothesis for kidney tumorigenesis have failed to establish the formation of formate as a key event for TCE-induced kidney tumors. Overall, although MOAs and key events for TCE-induced liver and kidney tumors have yet to be definitively established, these results support the likelihood that toxicity is due to multiple metabolites through several MOAs, none of which appear to be irrelevant to humans