Some properties of cocoa swollen-shoot virus

SUMMARY: Partially purified preparations of the Kofi Pare isolate of cocoa swollen-shoot virus (CSSV) were usually inactivated after 10 min. at 50°, but not after 10 min. at 45°. Infectivity of freshly made preparations was greatly lessened by diluting 1/10, and lost at 1/100. The infectivity of preparations increased after storage for 24 hr; after 96 hr infectivity was retained at 0--4° but soon lost at 25°. The virus survived freezing in vitro and, with some loss of infectivity, freezing in leaves and storage in leaves dried over aluminium oxide. Infective material was precipitated from dilute extracts by half saturation with ammonium sulphate at 25°; it appeared to be equally stable over the range pH 6 to pH 8. Preparations of the symptomatologically distinct Kofi Pare, Mampong, Dawa, Nsaba and Bosomuoso isolates all contained similar rod-shaped particles of size about 121 x 28 m/μ

Further studies on cocoa yellow mosaic virus

Cocoa yellow mosaic virus from Sierra Leone is readily transmitted by sap to many woody and herbaceous dicotyledonous plants. It is serologically related to wild cucumber mosaic virus and to turnip yellow mosaic virus, and has similar chemical and physical properties. It is stable when frozen or lyophilized, inactivated when kept for 10 min. at 65°, and is precipitated without loss of infectivity in a half-saturated ammonium sulphate solution. The particles appear to be 25 Mμ across when mounted in neutral phosphotungstate, and 29 Mμ when shadowed. All preparations contained infective nucleoprotein particles which sedimented at 108 S, and non-infective protein shells which sedimented at 49 S

A multidimensional view? Evaluating the different and combined contributions of diaries and interviews in an exploration of asexual identities and intimacies

This article evaluates the relative contributions of diaries and interviews in multiple methods qualitative research exploring asexual identities and intimacies. Differentiated by three core differences: reflective time-frame (the day just had/lifetime), context (alone/with researcher) and mode (written/verbal), these methods had the potential to generate a multidimensional view of our topics. Using five cases in which data from both interviews and diaries were collected, this article explores how the intermeshed issues of identity and intimacy were constructed in each method, as well as reflecting on what was gained by their combination. Our analysis leads us to conclude that multiple methods do not always produce a fuller or a more rounded picture of individual participants’ lives. Nevertheless, the decision to collect data using different strategies did increase our chances of finding a method that suited individual participants, whether in style or focus

Manometric studies of bracken [Pteridium aquilinum (L.) Kuhn] thiaminase

RESP-398

Latent phenolase in extracts of broad-bean (Vicia faba L.) leaves. 2. Activation by anionic wetting agents

RESP-390

The oxidation of indolyl-3-acetic acid by waxpod bean root sap and peroxidase systems

RESP-340

The oxidation of β-(3-indolyl)propionic acid and γ-(3-indolyl)-n-butyric acid by peroxidase and Mn(2+)

RESP-350

The partial purification of properties of a thiaminase from bracken [Pteridium aquilinum (L.) Kuhn]

RESP-384