Oral administration of Ren-shen-yang-rong-tang \u27ninjin\u27yoeito\u27 protects against hematotoxicity and induces immature erythroid progenitor cells in 5-Fluorouracil-induced anemia

金沢大学医薬保健研究域薬学系The purpose of this study was to investigate the efficacy of four different Japanese and Chinese herbal prescriptions, Ren-Shen-Yang-Rong-Tang (Ninjin\u27yoeito, NYT), Chai-Hu-Gui-Zhi-Gan-Jiang-Tang (Saikokeishikankyoto, SKKT), Si-Jun-Zi-Tang (Shikunshito, SKT) and Si-Wu-Tang (Shimotsuto, SMT), which are traditionally used for anemia and fatigue, against hematotoxicity in mice treated with 5-fluorouracil (5-FU). NYT 1100 mg kg-1 day-1 injected orally for 7 consecutive days before and after 5-FU injection significantly suppressed reductions in red blood cell, white blood cell and platelet counts in peripheral blood, and accelerated their recovery. Administration of SKKT also produced a slight but significant improvement in 5-FU-induced erythrocytopenia, whereas SMT and SKT could not prevent anemia. Oral injection of NYT also inhibited 5-FU-induced decreases in peripheral reticulocyte and bone marrow cell counts on day 10, and markedly hastened their recovery on day 20, in a dose-dependent manner. Erythroid progenitor colonies, such as colony forming units-erythroid and burst forming units-erythroid, formed by marrow cells from mice treated with 5-FU were significantly increased by oral administration of NYT. These findings suggest that NYT has the potential to protect against hematotoxicity, and also has hematopoietic activity, through stimulation of immature erythroid progenitor cell differentiation.出版者許諾要件により全文公開は2010年7月より

ラット肝ミトコンドリアMonoamine Oxidaseによる過酸化水素産生系に対する柴胡加龍骨牡蠣湯の影響

Saiko-ka-ryukotsu-borei-to (SRBT) is adapted for psychoneurosis,such as autonomic dystonia and sleep disorder. Is has been postulated that reactive oxygen species (ROSs) plays an important role in psychoneurosis arising from oxidative neurogeneration.In this study,effect of SRBT on the formation of hydrogen peroxide generated by rat liver mitochondria oxdase (MAO) was investigated by enzymatic colorimetric assay using 2,2\u27-Azino-bis(3-ethyl benzthiazoline-6-sulfonic acid) (ABTS) oxidation catalyzed by peroxidase in vitro.SRBT (1mg/ml) had exhibited notable inhibition on the coupling reaction of ABTS radical cation with a hydrogen peroxide formed by peroxidase,suggesting inhibitory effect on monoamine oxdase activity.Comparative determination of the consequence on MAO with the lacking of each crude drug blended in SRBT indicated that Scutellaria root should by the active constituent participated with this activity.In Addition,when the MAO-inhibitory activity was compared with the partition of Sctellaria root decoction,the ethyl acetate-soluble layer had found to be the most effective in comparison with the other layers.Wogonin and baicalein had been revealed the principal components in ethyl acetate-soluble layer of Scutellaria root by TLC.These results have suggested that flavonoids derived from Scutellaria root might be involved in the inhibitory activity of SRBT on rat liver MAO

マキバブラシノキ Callistemon rigidusのマトリックスメタロプロテアーゼ-2阻害作用

The influence of Callistemon rigidus (Myrtaceae) on rat lung activated matrix matalloproteinase-2 (MMP-2) was examined. Methanol extracts from stem and fruit showed inhibitory effects on MMP-2.Activation was induced by p-aminophenylmercuric acetate,and inhibition was measired by using sodium dodecyl sulfate polyacrylamid gel electrophoresis gelatin zymography.The IC50 values were 2.96 and 32.0mg/mL,respectively. Piceatannol was isolated as the active constituent from the stem of this plant,and its IC50 value was 757.1μM. The usefulness of Callistemon rigidus as a supply source of piceatannol is noted,and utility of piceatannol for the investigation of the inhibition of activated MMP-2 was also expected for the prevention and treatment of various diseases concerned with excess activation of MMP

ロズマリン酸の抗酸化作用とラット肺組織マトリックスメタロプロチアーゼ阻害作用について

Rosmarinic acid is an ester of caffeic acid and 3, 4-dihydroxyphenyllactic acid, being found to have a multitude of biological activities such as anti-inflammation, antimutagen and antioxidant. In order to establish the relationship between antioxidants and matrix metalloproteinase (MMP) -inhibitory activity, rosmarinic acid and caffeic acid were evaluated for their potential in 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity and blocking of MMP activity. Gelatinase activity was measured using the gelatin degradation and/or gelatin-zymography assay. From comparison of the different levels of DPPH radical scavenging activity, it was deduced that a crucial role in inhibition for MMP activity might play by an antioxidant activity. This comparative study is proposed as a basis for designing the enhanced antiproteolytic activities, and no or reduced side effects for use in hindering inflammation, cancer invasion or metastasis and angiogenesis in lung

In situ酵素組織化学法によるラット脳及び肝Monoamine Oxidase活性の検討

In situ quantitative histochemical analysis on monoamine oxidase (MAO) activity in rat brain and liver was performed for its region specificity. Nitro blue tetrazolium was used for the formazan producing reductive reaction caused by enzymatic degradation product of tryptamine as MAO substrate. A linear relationship was obserbed between the integrated MAO activity and the absorbance analyzed by PC-associated image analysis. The MAO-A inhibitor, clorgylin, inhibited the MAO activity dose-dependently in brain region and liver tissue in vitro and in situ analyses with well correlation. These results suggested that this method could be useful for the quantitative analysis of MAO activity in tissue sections, especially in the rat locus coeruleus that plays an important role in noradrenergic neurons

Antioxidant Flavonols and Phenolic Compounds from <i>Atraphaxis frutescens</i> and Their Inhibitory Activities against Insect Phenoloxidase and Mushroom Tyrosinase

Chemical investigation of the aerial parts of <i>Atraphaxis frutescens</i> resulted in the isolation of five 7-methoxyflavonols with pyrogallol B-ring moieties (<b>1</b>–<b>5</b>), a fisetinidol glucoside (<b>13</b>), and a benzyl glycoside (<b>18</b>), together with 26 known compounds including flavonoids, phenylpropanoid amides, anthraquinone glycosides, lignans, and a benzyl derivative. The principal chemical structural feature of the isolated compounds was either a pyrogallol or catechol B-ring moiety, and they showed potent 1,1-diphenyl-2-picrylhydrazyl radical scavenging activities. To assess the effects of these antioxidants on biological enzymes, their inhibitory effects against an insect phenoloxidase and a mushroom tyrosinase were evaluated. This study indicated that insect phenoloxidase was inhibited by phenylpropanoid amides and that mushroom tyrosinase was inhibited by the characteristic 7-methoxyflavonol 3-<i>O</i>-rhamnopyranosides