Oral, nasal and pharyngeal exposure to lipopolysaccharide causes a fetal inflammatory response in sheep.

BackgroundA fetal inflammatory response (FIR) in sheep can be induced by intraamniotic or selective exposure of the fetal lung or gut to lipopolysaccharide (LPS). The oral, nasal, and pharyngeal cavities (ONP) contain lymphoid tissue and epithelium that are in contact with the amniotic fluid. The ability of the ONP epithelium and lymphoid tissue to initiate a FIR is unknown.ObjectiveTo determine if FIR occurs after selective ONP exposure to LPS in fetal sheep.MethodsUsing fetal recovery surgery, we isolated ONP from the fetal lung, GI tract, and amniotic fluid by tracheal and esophageal ligation and with an occlusive glove fitted over the snout. LPS (5 mg) or saline was infused with 24 h Alzet pumps secured in the oral cavity (n = 7-8/group). Animals were delivered 1 or 6 days after initiation of the LPS or saline infusions.ResultsThe ONP exposure to LPS had time-dependent systemic inflammatory effects with changes in WBC in cord blood, an increase in posterior mediastinal lymph node weight at 6 days, and pro-inflammatory mRNA responses in the fetal plasma, lung, and liver. Compared to controls, the expression of surfactant protein A mRNA increased 1 and 6 days after ONP exposure to LPS.ConclusionONP exposure to LPS alone can induce a mild FIR with time-dependent inflammatory responses in remote fetal tissues not directly exposed to LPS

QZ Serpentis: A Dwarf Nova with a 2-Hour Orbital Period and an Anomalously Hot, Bright Secondary Star

We present spectroscopy and time-series photometry of the dwarf nova QZ Ser. The spectrum shows a rich absorption line spectrum of type K4 +- 2. K-type secondary stars are generally seen in dwarf novae with orbital periods P-orb around 6 h, but in QZ Ser the absorption radial velocities show an obvious modulation (semi-amplitude 207(5) km/s) at P-orb = 119.752(2) min, much shorter than typical for such a relatively warm and prominent secondary spectrum. The H-alpha emission-line velocity is modulated at the same period and roughly opposite phase. Time-series photometry shows flickering superposed on a modulation with two humps per orbit, consistent with ellipsoidal variation of the secondary's light. QZ Ser is a second example of a relatively short-period dwarf nova with a surprisingly warm secondary. Model calculations suggest that the secondary is strongly enhanced in helium, and had already undergone significant nuclear evolution when mass transfer began. Several sodium absorption features in the secondary spectrum are unusually strong, which may indicate that the present-day surface was the site of CNO-cycle hydrogen burning in the past.Comment: 11 pages, 3 postscript figures, 1 jpeg greyscale figure. Accepted for publication in PAS

XTE J2123-058: A New Neutron Star X-Ray Transient

We report on optical and RXTE observations of a new high-latitude bursting X-ray transient, XTE J2123-058. We identified the optical counterpart, measured the 5.9573 hr orbital period and constrained the binary inclination and the source distance. The distance lower limit indicates that the source is at least 2.6 kpc from the Galactic plane, which is unusual for an LMXB. RXTE observations were made between June and August 1998 during the first detected outburst from this source. We describe correlations between X-ray timing and spectral properties and discuss the possibility that the propeller mechanism turned on during the decay of the outburst. During one of the RXTE observations, we detect a pair of high frequency QPOs at 847.1 +/- 5.5 Hz and 1102 +/- 13 Hz simultaneously. According to the beat frequency model, the QPO separation implies a neutron star spin period near 3.9 ms. As the X-ray flux decreases at the end of the outburst, the amplitude of the optical modulation increases significantly. This behavior can be explained if the size of the accretion disk decreases during the decay of the outburst.Comment: 24 pages, 9 figures, accepted by Ap

Child relationships in the middle grades

Thesis (Ed.M.)--Boston Universit

CLIVAR Mode Water Dynamics Experiment (CLIMODE) fall 2005, R/V Oceanus voyage 419, November 9, 2005–November 27, 2005

CLIMODE (CLIVAR Mode Water Dynamic Experiment) is a program designed to understand and quantify the processes responsible for the formation and dissipation of North Atlantic subtropical mode water, also called Eighteen Degree Water (EDW). Among these processes, the amount of buoyancy loss at the ocean-atmosphere interface is still uncertain and needs to be accurately quantified. In November 2005, a cruise was made aboard R/V Oceanus in the region of the separated Gulf Stream, where intense oceanic heat loss to the atmosphere is believed to trigger the formation of EDW. During that cruise, one surface mooring with IMET meteorological instruments was anchored in the core of the Gulf Stream as well as two moored profilers on its southeastern edge. Surface drifters, APEX floats and bobby RAFOS floats were also deployed along with two other moorings with sound sources. CTD profiles and water samples were also carried out. This array of instruments will permit a characterization of EDW with high spatial and temporal resolutions, and accurate in-situ measurements of air-sea fluxes in the formation region. The present report documents this cruise, the instruments that were deployed and the array of measurements that was set in place.Funding was provided by the National Science Foundation under Grant No. OCE 04-24536

Cellular Models of Aggregation-Dependent Template-Directed Proteolysis to Characterize Tau Aggregation Inhibitors for Treatment of Alzheimer's Disease

Copyright © 2015, The American Society for Biochemistry and Molecular Biology. Acknowledgements-We thank Drs Timo Rager and Rolf Hilfiker (Solvias, Switzerland) for polymorph analyses.Peer reviewedPublisher PD