Limb Regeneration in Horseshoe Crabs

Horseshoe crabs, like many invertebrates, are able to regenerate ablated appendages. It was hypothesized that ependymin and/or juvenile hormone would contribute to regeneration at ablation sites and could be demonstrated by immunohistochemistry. Ablated appendages and regenerated stumps were examined by light and electron microscopy. The post-ablation tissue retracts into the remaining limb where new shell is formed in between two layers of living tissue. JH immunoreactivity was found in blood cells in the regenerating stumps. Anti-EPN immunoreactivity was not detected

Emergency Packaged Water Distribution System

This report, prepared for the Puerto Rican Aqueduct and Sewer Authority, documents the feasibility of implementing an emergency packaged water distribution system throughout Puerto Rico. The project analyzed all aspects of various bottled and bagged water packaging methods, including a thorough cost analysis of these methods and customer reactions to the proposed packaging types. Detailed analysis of federal regulations governing packaged water and packaging facilities were also included. A roadmap has been provided for the implementation of a packaged water system and recommendations on the use of such a system to supplement their current emergency system