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    2-D gel electrophoresis, silver staining and western blotting of <i>Toxocara canis</i> Excretory Secretory Antigens (TES-Ag).

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    <p>The TES-Ag sample was separated and analyzed using 2D gel electrophoresis and western blotting. Three of the 2DE gels were transferred to nitrocellulose membranes and probed with a strong EIA positive <i>Toxocara</i> human sera pool (A), a negative human serum sample (B), and <i>Baylisascaris procyonis</i> positive serum (C).A reference gel was stained using silver stain (D). The circled spots in D represent proteins that were excised and subjected to mass spectrometry analysis.</p
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