A comparison of dried shiitake mushroom in log cultivation and mycelial cultivation from different geographical origins using stable carbon and nitrogen isotope analysis

We determined carbon and nitrogen isotopic compositions (δ13C and δ15N) of dried shiitake mushroom (Lentinula edodes) samples from Japan, China, South Korea and Brazil in order to discriminate their geographical origins. In log cultivation, the δ13C values of Japanese dried shiitake samples were lower than those of Chinese samples, depending on the δ13C values of log and their growth conditions. In mycelial cultivation, the δ13C and δ15N values of Japanese dried shiitake samples were higher than those of Chinese samples. By using the δ13C and δ15N values, 87.4 ％ of Japanese dried shiitake samples (n＝95) and 87.9 ％ of Chinese dried shiitake samples (n＝66) in log cultivation, 90.0 ％ of the Japanese dried shiitake samples (n＝50) and 93.9 ％ of Chinese dried shiitake samples (n＝114) in mycelial cultivation, were correctly classified according to the production site. These results suggested that the δ13C and δ15N values will be potentially useful for tracing their geographical origin of dried shiitake samples. © 2015 The Japan Society for Analytical Chemistry

Relationship between Panus lecomtei and P. strigellus inferred from their morphological, molecular and biological characteristics

Panus strigellus was first recorded in Amazonas State of Brazil. This edible mushroom has macroscopic characteristics similar to those of P. lecomtei. In this study, we used evidence obtained from a morphological comparison, molecular analyses and mating tests to clarify the taxonomic status of P. strigellus and show differences compared to the sympatric species of Panus. In addition, this paper discusses and reports some morphological characteristics of P. strigellus. The tetrapolar mating system of P. strigellus and the incompatibility between P. lecomtei and P. strigellus was confirmed. The geographical distributions of both species in the Americas are presented. © 2015 The Mycological Society of Japan.Published by Elsevier B.V. All rights reserved

Rapid Species Identification of Cooked Poisonous Mushrooms by Using Real-Time PCR▿

Species-specific identification of the major cooked and fresh poisonous mushrooms in Japan was performed using a real-time PCR system. Specific fluorescence signals were detected, and no nonspecific signals were detected. Therefore, we succeeded in developing a species-specific test for the identification of poisonous mushrooms within 1.5 h