Agronomic biofortification of calcium in cabbage (Brassica Oleracea var capitata) applied with different sources of liming in Ca deficient acidic soil of Coonoor, The Nilgiris (Typic Dystropept)

The human body needs calcium (Ca) to maintain strong bones and teeth and to build a strong structure, helping muscles contract and playing a crucial role in the structural and signalling process. However, low calcium consumption in the diet has related to a variety of disorders in humans, which can have long-term health repercussions. Therefore, this study aimed to evaluate the Ca biofortification capacity of cabbage (Brassica oleracea var capitata) supplied with different Ca-supplying inorganic fertilizer sources at various fixed levels based on soil liming potential grown in open field conditions where four hybrids of cabbage grown in Ca deficient acidic soil. Ca applied as Limestone (CaCO3) (150% and 175% liming potential) and Dolomitic limestone [CaMg(CO3)2]150% liming potential yield high Ca content in cabbage head and foliage (61.3 mg 100 g-1), high glucosinolates content (53.12 mg 100 g-1) and lower oxalate(0.31 mg 100 g-1) that produced firmer head as compared with Ca untreated control which also promoted high market value for Ca biofortified ones. On the other hand, Ca addition leads to lower Fe and Mg content in the cabbage tissues due to an antagonistic effect. All four hybrids of cabbage studied using the agronomic method of biofortification significantly(p≤0.05) improved Ca enrichment (20% more compared to control) without showing any toxicity symptoms making possibility to obtain Ca biofortified cabbage in acidic soil of a hilly ecosystem by application of liming.

Fluorescence-Quenched Substrates for Live Cell Imaging of Human Glucocerebrosidase Activity

Deficiency of the lysosomal glycoside hydrolase glucocerebrosidase (GCase) leads to abnormal accumulation of glucosyl ceramide in lysosomes and the development of the lysosomal storage disease known as Gaucher’s disease. More recently, mutations in the GBA1 gene that encodes GCase have been uncovered as a major genetic risk factor for Parkinson’s disease (PD). Current therapeutic strategies to increase GCase activity in lysosomes involve enzyme replacement therapy (ERT) and molecular chaperone therapy. One challenge associated with developing and optimizing these therapies is the difficulty in determining levels of GCase activity present within the lysosomes of live cells. Indeed, visualizing the activity of endogenous levels of any glycoside hydrolases, including GCase, has proven problematic within live mammalian cells. Here we describe the successful modular design and synthesis of fluorescence-quenched substrates for GCase. The selection of a suitable fluorophore and quencher pair permits the generation of substrates that allow convenient time-dependent monitoring of endogenous GCase activity within cells as well as localization of activity within lysosomes. These efficiently quenched (∼99.9%) fluorescent substrates also permit assessment of GCase inhibition in live cells by either confocal microscopy or high content imaging. Such substrates should enable improved understanding of GCase in situ as well the optimization of small-molecule chaperones for this enzyme. These findings also suggest routes to generate fluorescence-quenched substrates for other mammalian glycoside hydrolases for use in live cell imaging

Comparative Performance of Planting Materials and Standardization of Nutrient Requirement for High Yield, Quality and Early Spike Development in Tuberose (Agave amica Medik.) cv. Arka Prajwal

The study was carried out at Vegetable Research Station, TNAU, Palur, Tamil Nadu during 2021to2022. The experiment was laid out in Factorial Randomized Block Design (FRBD) with eight treatments and three replications. Planting materials used for the study were bulbs and bulblets. The eight nutrient combinations were tried with foliar application of micronutrient like zinc sulphate (0.2%), Ferrous sulphate (0.5%), Copper sulphate (0.4%) and Boric acid (0.2%) on 30, 60 and 90 DAP, individually and in combination with 25 t ha-1 FYM and recommended dose of fertilizer (200:200:200 kg ha-1 NPK). The control was maintained with RDF. Various biometric observations on growth and flowering attributes of tuberose were observed for all the treatments at different stages. The result of the present investigation revealed that among the interaction effects,B1N8[RDF + ALL 19 (NPK) + GA3@ 200 ppm (2 spray at 30 days intervals) + ZnSO4 (0.2%) + FeSO4 (0.5%) + H3BO3 (0.2 %) + CuSO4 (0.4 %)] recorded the highest plant height (95.12 cm), leaf length (51.85 cm), leaf width (2.4 cm), number of leaves per plants (42.67), rachis length (34.87cm), number of floret/spikes (46.66), flower duration (18.12 days), floret length (6.92 cm) and floret diameter (4.39cm) followed by the treatment B2N8 which recorded the highest height (90.44 cm), leaf length (48.48 cm), leaf width (2.2 cm), number of leaves per plants (39.78), rachis length (30.42 cm), number of floret/spikes (44.95), flower duration (19.93days), floret length (6.45cm) and floret diameter (4.21cm). From the interaction effect, the treatment combinationB2N1 [control (RDF) 200:200:200 NPK] recorded the lowest plant height (72.36 cm), leaf length (42.81 cm), leaf width (1.12 cm) and number of leaves per plants (43.00), rachis length (25.11cm), number of floret/spikes (37.00), flower duration (15.23days), floret length (5.04 cm) and floret diameter (3.02 cm)