7,012 research outputs found
Temporal regulation of murine cytomegalovirus transcription and mapping of viral RNA synthesized at immediate early times after infection
The replication of murine cytomegalovirus strain Smith in murine embryonic fibroblasts was investigated at immediate early, early, and late times after infection. Cloned subgenomic HindIII fragments of murine cytomegalovirus DNA served to define the regions of transcription. At immediate early times viral RNA classes ranging in size from 5.1 to 1.05 kilobases (kb) were transcribed mainly from the fragments HindIII-K and -L, whereas low levels of transcription were detected from the two termini HindIII-E and HindIII-N. A characteristic pattern of proteins could be translated from immediate early RNA in vitro. At early and late times after infection transcription from all HindIII fragments occurred, but different patterns of transcripts and proteins could be identified. Inhibitors of DNA synthesis induced differences in the late transcription pattern, located in the HindIII-F fragment. The coding region for abundant immediate early transcription could be located at between 0.769 and 0.817 map units. A plasmic clone containing the main part (0.769 to 0.815 map units) of this region was constructed. This region coded for six polyadenylated immediate early RNA species of 5.1, 2.75, 2.0, 1.75, 1.65, and 1.05 kb in size. Only the 1.75-kb RNA originated entirely from the HindIII-L fragment. The 5.1- and 2.75-kb RNA species were encoded by both the HindIII-L and HindIII-K fragments, and the 2.0-, 1.65-, and 1.05-kb RNA species were entirely transcribed within HindIII-K
Location, transcripts, and translation products
Cloned genomic fragments from the region (0.769 to 0.818 map units) coding for immediate-early (IE) transcripts of murine cytomegalovirus (MCMV) were used to analyze the physical organization of this region, the direction of transcription, and the proteins synthesized in vitro. Three IE transcription units could be identified. From IE coding region 1 (ie1; 0.781 to 0.796 map units) a dominant 2.75-kilobase (kb) RNA was transcribed from right to left on the prototype arrangement of the MCMV genome which directed the synthesis of an 89,000-molecular-weight polypeptide (89K polypeptide), the major IE protein. This phosphoprotein (pp89) has been shown to be active in the regulation of transcription. Upstream of ie1 and separated by the MCMV enhancer sequence was a second IE coding region, ie2, which was mapped at 0.803 to 0.817 map units. From ie2 a 1.75-kb RNA of moderate abundance was transcribed in the direction opposite to that of the ie1 RNA. After hybrid selection of the ie2 transcript, a 43,000-molecular-weight translation product was detected. A third coding region, ie3, was located directly downstream of ie1 (0.773 to 0.781 map units). The series of RNAs with low abundance, terminating in ie3, probably used the ie1 transcription start site and ranged from 1.0 to 5.1 kb in size. The 5.1-kb RNA apparently represents the nonspliced transcript from both coding regions ie1 and ie3. A 15K polypeptide was translated in vitro from RNA that was hybrid selected by ie3 sequences. Immunoprecipitation with monoclonal antibody revealed that 31K to 67K polypeptides were related to pp89. Some of these proteins were translated from RNAs that were smaller than 2.75 kb. Polypeptides related to pp89 were also synthesized in vivo. Because polypeptides unrelated to pp89 that were translated from RNA that was selected by ie2 and ie3 sequences were not immunoprecipitated by murine antisera, we assumed that the amount of these proteins synthesized in vivo during infection was probably very lo
Baryon Interactions in Nuclear and Hypernuclear Matter
The production and structure of Λ hypernuclei are investigated in field
theoretical models. The production in coherent p+A reactions is investigated by
means of a resonance model. Results of exploratory calculations for associated
strangeness production are presented for proton reactions on 40Ca. The target
single particle wave functions are obtained from DDRH theory with density
dependent Dirac-Brueckner meson-baryon vertices. The dependence of the
in-medium vertices on density is discussed.Comment: 5 pages, 2 figs, proceedings for 'HADRON SPECTROSCOPY, Tenth
International Conference on Hadron Spectroscopy, Aschaffenburg, Germany
2003'. to be published in the AIP series of conference proceeding
Ledoux-Convection in Protoneutron Stars --- a Clue to Supernova Nucleosynthesis?
Two-dimensional hydrodynamical simulations of the deleptonization of a newly
formed neutron star were performed. Driven by negative lepton fraction and
entropy gradients, convection starts near the neutrinosphere about 20-30 ms
after core bounce, but moves deeper into the protoneutron star, and after about
one second the whole protoneutron star is convective. The deleptonization of
the star proceeds much faster than in the corresponding spherically symmetrical
model because the lepton flux and the neutrino luminosities increase by up to a
factor of two. The convection below the neutrinosphere raises the
neutrinospheric temperatures and mean energies of the emitted neutrinos by
10-20%. This can have important implications for the supernova explosion
mechanism and changes the detectable neutrino signal from the Kelvin-Helmholtz
cooling of the protoneutron star. In particular, the enhanced electron neutrino
flux relative to the electron antineutrino flux during the early post-bounce
evolution might solve the overproduction problem of certain elements in the
neutrino-heated ejecta in models of type-II supernova explosions.Comment: 17 pages, LaTeX, 8 postscript figures, uses epsf.sty. To appear in
ApJ 473 (Letters), 1996 December 1
Many-body Theory at Extreme Isospin
The structure of nuclei far off beta-stability is investigated by nuclear
many-body theory. In-medium interactions for asymmetric nuclear matter are
obtained by (Dirac-) Brueckner theory thus establishing the link of nuclear
forces to free space interactions. HFB and RPA theory is used to describe
ground and excited states of nuclei from light to heavy masses. In extreme
dripline systems pairing and core polarization are found to be most important
for the binding, especially of halo nuclei. The calculations show that far off
stability mean-field dynamics is gradually replaced by dynamical correlations,
giving rise to the dissolution of shell structures.Comment: 10 pages, 5 figures, to appear in the proceedings of Nuclear Physics
at the Borderline, NPBL2001, Lipari, Sicily, Italy, May 2001 (World
Scientific
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