The Grizzly, October 2, 1990

Books Stolen From Students and Professors: Suspect Arrested, 81 Books Confiscated • New Era of Recycling to Change Actions and Minds • Utilities Tunnel Nears Completion • Hardman\u27s Biography of Finney Turns Paperback • Gender Stereotypes by Dr. Englund • Speech Exemption Exam • Stolen Book List • Foreign Spotlight • Ghost Search Continues • AC/DC High Voltage Rock N\u27 Roll • Cop Rock Off-Key • Prince Premier • WVOU Schedule • Medieval Melodies Fill Forum • Homecoming Candidates • Vital Signs of the Trauma Center • Summer Science at Ursinus • Facelift for LSB • Bear Pack Wins Mets • Come Sailing! • Bears Lose 12-7 • Flag Football Kicks Off • Wagner Takes First in Mets Meet • US Must Aid Soviet Economic Woes • Temple Strike: Avoiding The Real Issue • Letters: Doughty Appreciates Grizzly; Wall Destruction Coincidental? • Soccer Working Hard • Hockey Splitshttps://digitalcommons.ursinus.edu/grizzlynews/1259/thumbnail.jp

The Grizzly, September 19, 1990

Election 1990: Hafer Speaks • Bon Voyage, Richter! • Meistersingers in England • Ursinus to Recycle? • Persian Gulf Dialog • Convocation 1990 • Ursinus Receives Japanese Grant • Slightly Steamed • The Phantoms of Ursinus • Ursinus Students Study in France • Red and Gold Hosts and Hostesses Needed • Berman Catalog Awarded • Urban Art • Bears Hound Hoyas in Opener • Field Hockey on the Ball • Bruins Club to Hold Fifth Bear Pack Run • Lady Bears Start Fast • Netters Improving • Score!! • Encourage Diversity • On Censoring Art • Not Oil Only • Prediction: War With Iraq • Going with the Flow • Bolt to Latest Discoverieshttps://digitalcommons.ursinus.edu/grizzlynews/1257/thumbnail.jp

A Bispecific Antibody Based Assay Shows Potential for Detecting Tuberculosis in Resource Constrained Laboratory Settings

The re-emergence of tuberculosis (TB) as a global public health threat highlights the necessity of rapid, simple and inexpensive point-of-care detection of the disease. Early diagnosis of TB is vital not only for preventing the spread of the disease but also for timely initiation of treatment. The later in turn will reduce the possible emergence of multi-drug resistant strains of Mycobacterium tuberculosis. Lipoarabinomannan (LAM) is an important non-protein antigen of the bacterial cell wall, which is found to be present in different body fluids of infected patients including blood, urine and sputum. We have developed a bispecific monoclonal antibody with predetermined specificities towards the LAM antigen and a reporter molecule horseradish peroxidase (HRPO). The developed antibody was subsequently used to design a simple low cost immunoswab based assay to detect LAM antigen. The limit of detection for spiked synthetic LAM was found to be 5.0 ng/ml (bovine urine), 0.5 ng/ml (rabbit serum) and 0.005 ng/ml (saline) and that for bacterial LAM from M. tuberculosis H37Rv was found to be 0.5 ng/ml (rabbit serum). The assay was evaluated with 21 stored clinical serum samples (14 were positive and 7 were negative in terms of anti-LAM titer). In addition, all 14 positive samples were culture positive. The assay showed 100% specificity and 64% sensitivity (95% confidence interval). In addition to good specificity, the end point could be read visually within two hours of sample collection. The reported assay might be used as a rapid tool for detecting TB in resource constrained laboratory settings

Mouse Chromosome 11

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/46996/1/335_2004_Article_BF00648429.pd