Application of Digital (Motic) and Scanning electron microscope in histological study of leaf of Tecoma gaudichaudi DC (Bignoniaceae)

Tecoma gaudichaudi DC (Bignoniaceae) is a shrub or a small tree found in various regions of Maharashtra. The objective of the study was to develop various standardization parameters for the evaluation of leaves of this plant. Microscopy, powder characteristics and scanning electron microscopy (SEM) of leaves were observed and results were recorded. Histological study of leaves shows underlined palisade cells on the upper epidermal surface, anomocytic stomata on the lower epidermis with covering (2–3 cells) and glandular trichomes etc. The specific observed characteristics such as types of stomata and trichomes were predominantly observed on the lower epidermal surface of the leaf. Physicochemical analysis such as extractive value includes petroleum ether, ethanol, ethyl acetate and aqueous soluble extractive values of 3.08, 10.50, 8.5, and 13.4% w/w respectively; extracts were analysed by chemical test and showed presence of flavonoids, tannins, steroids and triterpenoids etc. Fluorescence analysis is one of the parameters that help in the analysis of chemical constituents. So these parameters are useful in the authentication of T. gaudichaudi DC and can play a vital role in authentication and standardization of botanicals

Dataset on leaf surface and elemental study of four species of Bignoniaceae family by SEM-EDAX

The data presented in this article are related to the scanning electron microscope and elemental studies in the four species of Bignoniaceae namely Tecoma gaudichaudi DC (Sample 1), Tecoma capensis (Thunb.) Lindl. (Sample 2), Tecoma stans (L.) Juss.Ex Kunth (Sample 3), Tabebuia rosea (Bertol.) (Sample 4). The SEM images were obtained for permanent record. The abaxial and adaxial surfaces of each species were carefully studied. In addition to this, the consistent occurrence of anomocytic stomata in all four species of this family shows that morphological and taxonomically all the species are very close and intimate.The elemental data on leaf samples of all four species were performed and total eight important components were present such as C, O, Mg, Al, Si, Cl, K, Ca. These elements are useful, so identification of inorganic components of these species defiantly helps to promote as dietary elements. Keywords: Bignoniaceae, Tecoma, Tabebuia, Tecoma gaudichaudi DC, Tecoma capensis (Thunb.) Lindl, Tecoma stans (L.) Juss.ex Kunth, Tabebuia rosea (Bertol), Scanning electron microscopy, Elemental analysi

A validated HPTLC method for the quantification of friedelin in Putranjiva roxburghii Wall extracts and in polyherbal formulations

In present study HPTLC method was developed and validated for the determination of friedelin in Putranjiva roxburghii Wall (family: Euphorbiaceae) leaf, bark extract and in polyherbal formulations. Analysis of samples were performed on TLC aluminium precoated plate (60 F254) by using mobile phase toluene: chloroform (9:1 v/v). Plate was derivatized with vanillin sulphuric acid and scanned at 580 nm. Developed method found to give compact spot for friedelin at Rf value 0.43 ± 0.01. The method was validated using International Council for Harmonization (ICH) guidelines including linearity, precision, accuracy, and robustness. Friedelin was found to be present in leaf extract of Putranjiva roxburghii Wall (0.003% w/w), in bark (0.04% w/w), formulation 1 (0.002% w/w) and formulation 2 (0.035% w/w). A good linearity relationship was found to be (100–500 ng spot−1) with correlation coefficient (r2) value of 0.9892 for friedelin. Limit of detection and limit of quantitation was found to be 32.15, 97.44 ng/band respectively for friedelin. The developed method was found to be accurate and precise with 0.78%, 0.9% (%RSD) for interday and intraday precision. Accuracy of the method was performed by recovery studies at three different concentration levels and the average percentage recovery was found to be 98.55% for friedelin. The proposed method for the quantitation of friedelin was found to be simple, specific, accurate and robust in Putranjiva roxburghii Wall and polyherbal formulations

Data on investigation of hypoglycemic, anti-cholesteremic, in vivo antioxidant and pancreatic beta cell protective effect of Putranjiva roxburghii Wall bark in streptozotocin-induced diabetic rats

The data existing in this article are associated to the antidiabetic activity of ethyl acetate extract of Putranjiva roxburghii Wall barks (EAPR) in streptozotocin (STZ) induced diabetic rats at a dose of 250 & 500 mg/kg by oral route for 21 days. The phytochemical screening of the extract was carried out by gas chromatography and mass spectrometry. Diabetes was induced by streptozotocin (50 mg/kg; i.p), EAPR (250 & 500 mg/kg; b.wt) and standard Insulin (6 IU/animal; subcutaneous; o.i.d) were administered to the diabetic rats. Body weight and blood glucose were estimated weekly. Cholesterol, SOD and CAT were estimated in the blood serum on 21 days of the investigation period. Oral administration of EAPR (500 mg/kg) significant rises in the body weight, decrease in the blood glucose and total cholesterol and restore function of SOD and CAT enzymes (P < 0.05). Current data were also supported by histological study, necrosis was observed in the diabetic rat pancreas; however, necrosis was less observable in treated groups. These findings reveal that an ethyl acetate extract of Putranjiva roxburghii Wall barks shows antihyperglycemic, anti-cholesterolemic, antioxidant and improved the cell density of β-cells of islets of Langerhans in diabetic rats. Keywords: Putranjiva roxburghii Wall barks, Streptozotocin, Antihyperglycemic, Anti-cholesterolemic, Antioxidan