Ｘ線のスペクトル/タイミング解析による白鳥座X-1のブラックホールスピンの研究

広島大学(Hiroshima University)博士(理学)Doctor of Sciencedoctora

Construction of microbial platform for an energy-requiring bioprocess: practical 2′-deoxyribonucleoside production involving a C−C coupling reaction with high energy substrates

BACKGROUND: Reproduction and sustainability are important for future society, and bioprocesses are one technology that can be used to realize these concepts. However, there is still limited variation in bioprocesses and there are several challenges, especially in the operation of energy-requiring bioprocesses. As an example of a microbial platform for an energy-requiring bioprocess, we established a process that efficiently and enzymatically synthesizes 2′-deoxyribonucleoside from glucose, acetaldehyde, and a nucleobase. This method consists of the coupling reactions of the reversible nucleoside degradation pathway and energy generation through the yeast glycolytic pathway. RESULTS: Using E. coli that co-express deoxyriboaldolase and phosphopentomutase, a high amount of 2′-deoxyribonucleoside was produced with efficient energy transfer under phosphate-limiting reaction conditions. Keeping the nucleobase concentration low and the mixture at a low reaction temperature increased the yield of 2′-deoxyribonucleoside relative to the amount of added nucleobase, indicating that energy was efficiently generated from glucose via the yeast glycolytic pathway under these reaction conditions. Using a one-pot reaction in which small amounts of adenine, adenosine, and acetone-dried yeast were fed into the reaction, 75 mM of 2′-deoxyinosine, the deaminated product of 2′-deoxyadenosine, was produced from glucose (600 mM), acetaldehyde (250 mM), adenine (70 mM), and adenosine (20 mM) with a high yield relative to the total base moiety input (83%). Moreover, a variety of natural dNSs were further synthesized by introducing a base-exchange reaction into the process. CONCLUSION: A critical common issue in energy-requiring bioprocess is fine control of phosphate concentration. We tried to resolve this problem, and provide the convenient recipe for establishment of energy-requiring bioprocesses. It is anticipated that the commercial demand for dNSs, which are primary metabolites that accumulate at very low levels in the metabolic pool, will grow. The development of an efficient production method for these compounds will have a great impact in both fields of applied microbiology and industry and will also serve as a good example of a microbial platform for energy-requiring bioprocesses

Percutaneous Anorectoplasty (PARP)-An Adaptable, Minimal-Invasive Technique for Anorectal Malformation Repair

Background: Anorectal malformations comprise a broad spectrum of disease. We developed a percutaneous anorectoplasty (PARP) technique as a minimal-invasive option for repair of amenable types of lesions. Methods: Patients who underwent PARP at five institutions from 2008 through 2021 were retrospectively analyzed. Demographic information, details of the operative procedure, and perioperative complications and outcomes were collected. Results: A total of 10 patients underwent the PARP procedure during the study interval. Patients either had low perineal malformations or no appreciable fistula. Most procedures were guided by ultrasound, fluoroscopy, or endoscopy. Median age at PARP was 3 days (range 1 to 311) days;eight patients were male. Only one intraoperative complication occurred, prompting conversion to posterior sagittal anorectoplasty. Functional outcomes in most children were highly satisfactory in terms of continence and functionality. Conclusions: The PARP technique is an excellent minimal-invasive alternative for boys born with perineal fistulae, as well as patients of both sexes without fistulae. The optimal type of guidance (ultrasound, fluoroscopy, or endoscopy) depends on the anatomy of the lesion and the presence of a colostomy at the time of repair

キャリア ケイセイ シエン キョウイク プログラム ノ カイハツ ト キョウイク コウカ ノ ヒョウカ : チーム イリョウ タイケン ガクシュウ ノ ジッセンテキ ケンキュウ オ トオシテ

我々は歯学部学生のキャリア形成支援のための教育プログラム「チーム医療体験学習」を開発し， 歯学部の2，3，4年次の学生を対象として実施した。本プログラムは，グループワークによる事前学 習とチーム医療の現場体験を2つの柱とし，学生の歯科医療職としてのキャリア意識を強化することを 目的とした。プログラム終了時のアンケートから，学生には歯科専門職としての将来の姿勢や目指す方 向性が具体化すると同時に幅広い知識を身につけたいといった学習に対する動機付が強化されるなど 明らかな教育成果が認められた。プログラム実施前後のアンケートの分析結果から， 「チーム医療体験 学習」が歯学部学生のキャリア形成支援教育の手法として有効なプログラムである可能性が示唆された。We prepared career advancement program “Experience learning in medical team treatment" towards the second，third and fouth year students of dental school. This program consits of prior learning concerned with 'Team treatment and Dental profession' and experience learning in medical team treatment at Tokushima University Hospital. The purpose of this program was to rouse career conciousness as a dental profession. This program provided students with career image which they want to be in the future. Besides，they were motivated to study more widely to approach their own image. In this paper，we evaluated the effects of the program“The experience learning in medical team treatment" on career advancement of dental school students. As a result，it is suggested that this program might be fruitful as career advancement on dental students