Kinetics of charge transfer in DNA containing a mismatch

Charge transfer (CT) in DNA offers a unique approach for the detection of a single-base mismatch in a DNA molecule. While the single-base mismatch would significantly affect the CT in DNA, the kinetic basis for the drastic decrease in the CT efficiency through DNA containing mismatches still remains unclear. Recently, we determined the rate constants of the CT through the fully matched DNA, and we can now estimate the CT rate constant for a certain fully matched sequence. We assumed that further elucidating of the kinetics in mismatched sequences can lead to the discrimination of the DNA single-base mismatch based on the kinetics. In this study, we investigated the detailed kinetics of the CT through DNA containing mismatches and tried to discriminate a mismatch sequence based on the kinetics of the CT in DNA containing a mismatch

DNAの構造制御に関する化学的研究とZ-DNA特異的反応

京都大学0048新制・課程博士博士(工学)甲第7866号工博第1846号新制||工||1144(附属図書館)UT51-99-G460京都大学大学院工学研究科合成・生物化学専攻(主査)教授 齋藤 烈, 教授 吉田 潤一, 教授 北川 進学位規則第4条第1項該当Doctor of EngineeringKyoto UniversityDFA