More calculations about deterrence

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/68274/2/10.1177_002200276500900104.pd

Extracting gene expression patterns and identifying co-expressed genes from microarray data reveals biologically responsive processes

Abstract Background A common observation in the analysis of gene expression data is that many genes display similarity in their expression patterns and therefore appear to be co-regulated. However, the variation associated with microarray data and the complexity of the experimental designs make the acquisition of co-expressed genes a challenge. We developed a novel method for Extracting microarray gene expression Patterns and Identifying co-expressed Genes, designated as EPIG. The approach utilizes the underlying structure of gene expression data to extract patterns and identify co-expressed genes that are responsive to experimental conditions. Results Through evaluation of the correlations among profiles, the magnitude of variation in gene expression profiles, and profile signal-to-noise ratio's, EPIG extracts a set of patterns representing co-expressed genes. The method is shown to work well with a simulated data set and microarray data obtained from time-series studies of dauer recovery and L1 starvation in C. elegans and after ultraviolet (UV) or ionizing radiation (IR)-induced DNA damage in diploid human fibroblasts. With the simulated data set, EPIG extracted the appropriate number of patterns which were more stable and homogeneous than the set of patterns that were determined using the CLICK or CAST clustering algorithms. However, CLICK performed better than EPIG and CAST with respect to the average correlation between clusters/patterns of the simulated data. With real biological data, EPIG extracted more dauer-specific patterns than CLICK. Furthermore, analysis of the IR/UV data revealed 18 unique patterns and 2661 genes out of approximately 17,000 that were identified as significantly expressed and categorized to the patterns by EPIG. The time-dependent patterns displayed similar and dissimilar responses between IR and UV treatments. Gene Ontology analysis applied to each pattern-related subset of co-expressed genes revealed underlying biological processes affected by IR- and/or UV- induced DNA damage. Conclusion EPIG competed with CLICK and performed better than CAST in extracting patterns from simulated data. EPIG extracted more biological informative patterns and co-expressed genes from both C. elegans and IR/UV-treated human fibroblasts. Using Gene Ontology analysis of the genes in the patterns extracted by EPIG, several key biological categories related to p53-dependent cell cycle control were revealed from the IR/UV data. Among them were mitotic cell cycle, DNA replication, DNA repair, cell cycle checkpoint, and G0-like status transition. EPIG can be applied to data sets from a variety of experimental designs

Note and Comment

Power of the US Supreme Court to Enforce Judgments Against States - In the year 1460, when the perogatives of sovereignty or at least of the Crown were asserted in England much more vigorously than they are today, the Counseill of the right high and mighty Prynce Richard Duc of York, brought into the Parliament Chambre a writyng conteignyng the clayme and title of the right, that the seid Duc pretended unto the Corones of Englond and of Fraunce, and Lordship of Trelond, and the same writyng delyvered to the Right Reverent Fader in God George Bishop of Excestre, Chaunceller of Englond, desiryng hym that the same writyng myght be opened to the Lordes Spirituelx and Temporelx assembled in this present Parlement, and that the seid Duc myght have brief and expedient answere thereof. Whereupon the lords, apparently embarrassed by this extraordinary manifestation of confidence in them, declared that the said writyng shuld be radde and herd, not to be answered without the Kyngs commaundement, for so moche as the mater is so high, and of soo grete wyght and poyse.\u27 Vhen four days later the petition was again urgently presented therupon incontynent all the seid Lordes Spirituelx and Temporelx went to the Kyngs high presence, and therunto opened and declared the seid mater, by the mouth of his said Chaunceller of Englond. The King was graciously pleased to command the lords that they should serche for to fynde in asmuch as in them was, all such thyngs as myght be objecte and leyde ayenst the cleyme and title of the seid Duc. And though the King\u27s command could scarcely be regarded as indicating a judicial inquiry, the lords in their extremity sent for the Kyngs Justices into the Parlement Chambre, to have their avis and Counsell in this behalf, * * * * sadly to take avisament therin, and to serche and fynde all such objections as myght be leyde ayenst the same, in fortefying of the Kynges right. Duke of York\u27s Claim to the Crown, 5 Rot. ParI., 375, I Wambaugh\u27s Cas. Const. Law, I

Clinical outcomes of ultrasound-assisted coagulation monitoring-adjusted catheter-directed thrombolysis for acute pulmonary embolism

BACKGROUND Ultrasound-assisted catheter-directed thrombolysis (USAT) may reverse right ventricular dysfunction due to acute pulmonary embolism (PE) with a favorable safety profile. METHODS We studied intermediate-high- and high-risk acute PE patients who underwent USAT at the University Hospital Zurich, 2018-2022. The USAT regimen included alteplase 10 mg per catheter over 15 h, therapeutic-dosed heparin, and dosage adaptations based on routinely monitored coagulation parameters, notably anti-factor Xa activity and fibrinogen. We focused on the mean pulmonary arterial pressure (mPAP) and the National Early Warning Score (NEWS) before and after USAT, and reported the incidence of hemodynamic decompensation, PE recurrence, major bleeding, and death over 30 days. RESULTS We included 161 patients: 96 (59.6 %) were men and the mean age was 67.8 (SD 14.6) years. Mean PAP decreased from a mean of 35.6 (SD 9.8) to 25.6 (SD 8.2) mmHg, whereas the NEWS decreased from a median of 5 (Q1-Q3 4-6) to 3 (Q1-Q3 2-4) points. No cases of hemodynamic decompensation occurred. One (0.6 %) patient had an episode of recurrent PE. Two (1.2 %) major bleeding events occurred, including one (0.6 %) intracranial, fatal hemorrhage in a patient with high-risk PE, severe heparin overdosing, and a recent head trauma (with negative CT scan of the brain performed at baseline). No other deaths occurred. CONCLUSIONS USAT resulted in a rapid improvement of hemodynamic parameters among patients with intermediate-high risk acute PE and selected ones with high-risk acute PE, without any recorded deaths related to PE itself. A strategy including USAT, therapeutic-dosed heparin, and routinely monitored coagulation parameters may partly explain the overall very low rate of major bleeding

Scholarship and security policy: a review of recent literature

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/68058/2/10.1177_002200275900300408.pd

Dry matter yields and hydrological properties of three perennial grasses of a semi-arid environment in east Africa

Enteropogon macrostachyus (Bush rye), Cenchrus ciliaris L. (African foxtail grass) and Eragrostis superba Peyr (Maasai love grass) are important perennial rangeland grasses in Kenya. They provide an important source of forage for domestic livestock and wild ungulates. These grasses have been used extensively to rehabilitate denuded patches in semi-arid environment of Kenya. This study investigated the dry matter yields and hydrological properties of the three grasses under simulated rainfall at three phenological stages; early growth, elongation and reproduction. Laboratory seed viability tests were also done. Hydrological properties of the three grasses were estimated using a Kamphorst rainfall simulator. Results showed that there was a significant difference (p > 0.05) in dry matter yields and soil hydrological properties at the different grass phenological stages. Generally, all the three grasses improved the soil hydrological properties with an increase in grass stubble height. C. ciliaris gave the best soil hydrological properties followed by E. macrostachyus and E. superba, respectively. E. macrostachyus recorded the highest seed viability percentage. C. ciliaris and E. superba were ranked second and third, respectively. C. ciliaris yielded the highest biomass production at the reproductive stage followed by E. superba and E. macrostachyus, respectively. (Résumé d'auteur