The effect of plyometric exercise on bone turnover markers and osteokines in younger and older women

The effect of a single-bout of plyometric exercise on markers of bone turnover and Wnt signalling-related osteokines was studied in 20 younger, pre-menopausal women (23.142.33 years) and 20 older, post-menopausal women (57.904.35 years of age). Blood samples were obtained at rest (i.e., pre-exercise) and 5 min, 1h, and 24h post- exercise and were analyzed for C-terminal crosslinking telopeptides of type I collagen (CTX), osteoprotegerin (OPG), sclerostin and dickkopf-1 (DKK-1), and estradiol. Resting levels of CTX, OPG, and sclerostin were significantly higher while DKK-1 and estradiol were significantly lower in older compared to younger women. CTX was higher at 5 min post-exercise compared to pre-exercise in younger women (326.027.0 vs. 292.029.0 pg/mL; p=0.049); however, no response was seen in older women. Sclerostin significantly decreased from 5 min (319.934.6 pg/mL) to 1h post-exercise (245.329.5 pg/mL) but increased between 1h and 24h post-exercise (368.333.9 pg/mL) only in younger women. DKK-1 decreased in both groups. In younger women, the decrease was continuous from 5 min (2560.20120.65 pg/mL) to 24h post-exercise (2176.60115.29 pg/mL, p=0.006). In post-menopausal women, the decrease was between pre-exercise (1949.69177.95 pg/mL) and 1h post-exercise (1549.82187.11 pg/mL, p=0.001) but returning to near pre-exercise levels 24h post-exercise. In the older women, OPG also decreased from pre-exercise (535.8 36.8 pg/mL) to 5 min post-exercise (475.1 39.0 pg/mL; p=0.048) and remained lower than baseline for up to 24h post-exercise (505.032.4 pg/mL; p=0.046). No changes were seen in the younger women. These results suggest that in women, one session of plyometric exercise is sufficient to induce significant changes in bone turnover and Wnt signalling related osteokines, however, the timing of the response varies significantly between age groups

Osteokines and Bone Markers at Rest and following Plyometric Exercise in Pre- and Postmenopausal Women

The effect of plyometric exercise on bone biomarkers has been studied in pediatric and young adult populations in order to better understand how exercise influences bone homeostasis. However, there are no such data in postmenopausal women, a group characterized by an uncoupling of the bone resorption-formation cycle. This study examined the serum concentrations of sclerostin, dickkopf-1 (DKK1), c-terminal crosslinking telopeptides of type I collagen (CTXI), and procollagen type I amino-terminal propeptide (PINP) at rest and following a single bout of plyometric exercise in 20 premenopausal (23.1±2.3 years) and 20 postmenopausal women (57.9±4.3 years). The exercise consisted of 128 jumps, organized into 5 circuit stations. Blood samples were obtained prior to and 5 min, 1 h, and 24 h postexercise. At rest, postmenopausal women had significantly higher sclerostin and CTXI, but lower DKK1 than premenopausal women. Sclerostin increased 5 min postexercise only in the premenopausal group. DKK1 decreased 24 h postexercise in the premenopausal women while it decreased 1 h postexercise in the postmenopausal women. In both groups, CTXI did not change across time and PINP decreased 5 min and 1 h postexercise (p<0.05). The PINP/CTXI ratio decreased 5 min and 1 h postexercise then significantly increased 24 h postexercise only in premenopausal women. These results indicate that although plyometric exercise is effective in eliciting osteoanabolic effects in younger women; such an effect is not evident in postmenopausal women

Multistudy Research Operations in the ICU: An Interprofessional Pandemic-Informed Approach

OBJECTIVES:. Proliferation of COVID-19 research underscored the need for improved awareness among investigators, research staff and bedside clinicians of the operational details of clinical studies. The objective was to describe the genesis, goals, participation, procedures, and outcomes of two research operations committees in an academic ICU during the COVID-19 pandemic. DESIGN:. Two-phase, single-center multistudy cohort. SETTING:. University-affiliated ICU in Hamilton, ON, Canada. PATIENTS:. Adult patients in the ICU, medical stepdown unit, or COVID-19 ward. INTERVENTIONS:. None. MEASUREMENTS AND MAIN RESULTS:. An interprofessional COVID Collaborative was convened at the pandemic onset within our department, to proactively coordinate studies, help navigate multiple authentic consent encounters by different research staff, and determine which studies would be suitable for coenrollment. From March 2020 to May 2021, five non-COVID trials continued, two were paused then restarted, and five were launched. Over 15 months, 161 patients were involved in 215 trial enrollments, 110 (51.1%) of which were into a COVID treatment trial. The overall informed consent rate (proportion agreed of those eligible and approached including a priori and deferred consent models) was 83% (215/259). The informed consent rate was lower for COVID-19 trials (110/142, 77.5%) than other trials (105/117, 89.7%; p = 0.01). Patients with COVID-19 were significantly more likely to be coenrolled in two or more studies (29/77, 37.7%) compared with other patients (13/84, 15.5%; p = 0.002). Review items for each new study were collated, refined, and evolved into a modifiable checklist template to set up each study for success. The COVID Collaborative expanded to a more formal Department of Critical Care Research Operations Committee in June 2021, supporting sustainable research operations during and beyond the pandemic. CONCLUSIONS:. Structured coordination and increased communication about research operations among diverse research stakeholders cultivated a sense of shared purpose and enhanced the integrity of clinical research operations