Effects of thymoquinone on alpha-amanitin induced hepatotoxicity in human C3A hepatocytes

Thymoquinone (TQ) has shown hepatoprotective effects in various experimental studies. We aimed to investigate the possible beneficial effects of TQ regarding its prevention of alpha-amanitin induced hepatotoxicity in human C3A hepatocytes. After administering alpha-amanitin in a concentrations of 1 and 10µg/mL on the cells in a hepatocyte cell line, TQ was administered in various concentrations (10, 5, 1, 0.5, 0.1, 0.05, 0.01, 0.005 µg/mL). The MTT test was used to determine cell viability. For the groups given only TQ at various concentrations, the cell viability rates at 48 hours post-administration were found at 82.6, 98.3, 102.1, 102.5, 99.4, 99.4, 101.9 and 106.3%, respectively. For the group with 1μg/mL alpha-amanitin and various TQ concentrations, the cell viability rates were found at 74.6, 88.5, 87.4, 88.7, 85.7, 86.8, 88.4, and 92.9%, respectively. For the group with 10μg/mL alpha-amanitin and various TQ concentrations, the cell viability rates for each TQ subgroup were found at 65.2, 79.2, 81.4, 81.1, 81.8, 81.8, 82.2 and 91.9%, respectively. Our study is the first in vitro study that investigates TQ’s effects on alpha-amanitin induced hepatotoxicity. Although TQ had beneficial effect in low doses did not significantly increase cell viability in liver damage due to alpha-amanitin toxicity

Anaphylaxis after intravenous infusion of dexketoprofen trometamol

Dexketoprofen trometamol (DT), a nonsteroidal anti-inflammatory drug, is a highly water-soluble salt and active enantiomer of rac-ketoprofen. Its parenteral form is commonly used for acute pain management in emergency departments of our country. Side effects such as diarrhea, indigestion, nausea, stomach pain, and vomiting may be seen after the use of DT. Anaphylactic shock (AS) secondary to infusion of DT is very rare and, to our knowledge, it is the first case report describing this side effect. This case report was presented to emphasize that AS may be seen after the use of DT. Keywords: Anaphylactic shock, Dexketoprofen trometamol, Intravenous infusion (MeSH database

ASSESSMENT OF THE USABILITY OF ULTRASONOGRAPHY BY EMERGENCY PHYSICIANS IN THE DIAGNOSIS OF ACUTE CHOLECYSTITIS

WOS: 000335937900037Background: Early diagnosis of acute stony cholecystitis allows rapid treatment and reduces mortality and morbidity. In this paper, we aimed to assess the competence of emergency medicine physicians in the ultrasonographic diagnosis of gallbladder pathologies in patients presenting at emergency departments with upper right quadrant pain. Methods: One hundred and sixty-eight patients over the age of 18 years presenting at the Emergency Medicine clinic on January 1 to July 1, 2013, were included in this prospective study. Ultrasonography (USG) of the upper right quadrant was applied to all patients at emergency services and the radiology unit. The results were statistically analyzed with the radiology clinic reports accepted as the gold standard. Results: With the radiology reports considered to be the gold standard, emergency physicians were able to identify the sonographic Murphy sign with 98.2% accuracy (95% confidence interval [CI] of 96.2-100), positive gallstones at 89.9% (95% CI 85.3-94.4), pericholecystic fluid at 89.3% (95% CI 84.6-94.0), and finally acute stony cholecystitis diagnosis at 98.2% (95% CI 96.2-100). Conclusion: Emergency medicine physicians may perform gallbladder USG procedures with similar results to those performed by radiology physicians following standardized training. Thus, USG may assume a function similar to the stethoscope as part of physical examination aiming toward clinical decisions with respect to the assessment of gallbladder diseases, and the decision to discharge patients from the emergency service of hospitalization in the clinic

Comparison of success and pain levels of supination-flexion and hyperpronation maneuvers in childhood nursemaid's elbow cases

Objective: The aim of this study was to compare the hyperpronation (HP) and the supination-flexion (SF) reduction techniques for reducing nursemaid's elbow in terms of efficacy and pain

Comparison of success and pain levels of supination-flexion and hyperpronation maneuvers in childhood nursemaid's elbow cases

Objective: The aim of this study was to compare the hyperpronation (HP) and the supination-flexion (SF) reduction techniques for reducing nursemaid's elbow in terms of efficacy and pain

Seasonal variations of patients presenting dyspnea to emergency departments in Europe: Results from the EURODEM Study

International audienc

The emergency department arrival mode and its relations to ED management and 30-day mortality in acute heart failure: an ancillary analysis from the EURODEM study

Background Acute heart failure patients are often encountered in emergency departments (ED) from 11% to 57% using emergency medical services (EMS). Our aim was to evaluate the association of EMS use with acute heart failure patients’ ED management and short-term outcomes. Methods This was a sub-analysis of a European EURODEM study. Data on patients presenting with dyspnoea were collected prospectively from European EDs. Patients with ED diagnosis of acute heart failure were categorized into two groups: those using EMS and those self-presenting (non- EMS). The independent association between EMS use and 30-day mortality was evaluated with logistic regression. Results Of the 500 acute heart failure patients, with information about the arrival mode to the ED, 309 (61.8%) arrived by EMS. These patients were older (median age 80 vs. 75 years, p  30/min in 17.1% patients vs. 7.5%, p = 0.005). The only difference in ED management appeared in the use of ventilatory support: 78.3% of EMS patients vs. 67.5% of non- EMS patients received supplementary oxygen (p = 0.007), and non-invasive ventilation was administered to 12.5% of EMS patients vs. 4.2% non- EMS patients (p = 0.002). EMS patients were more often hospitalized (82.4% vs. 65.9%, p < 0.001), had higher in-hospital mortality (8.7% vs. 3.1%, p = 0.014) and 30-day mortality (14.3% vs. 4.9%, p < 0.001). The use of EMS was an independent predictor of 30-day mortality (OR = 2.54, 95% CI 1.11–5.81, p = 0.027). Conclusion Most acute heart failure patients arrive at ED by EMS. These patients suffer from more severe respiratory distress and receive more often ventilatory support. EMS use is an independent predictor of 30-day mortality