Field of View Based Optimization of Aspheric Designed Geometric-Phase Doublet Lenses

Here we study aspheric phase pattern geometric-phase lenses through the design of doublet lenses in Zemax Optic Studio and in MATLAB using a simple ray tracing code. The solutions developed use variable spacer distances and are solved monochromatically (at 633 nm) and polychromatically (at 450, 532, and 633 nm). After selecting a Zemax generated polychromatically optimized doublet solution, we fabricated the lens utilizing liquid crystal films and direct writing. We characterized the liquid crystal alignment quality, efficiencies, and spot performance of this lens in comparison to a standard spherical singlet of the same back focal length and diameter (24.5 mm diameter and 40 mm back focal length at 633 nm). With this aspheric lens profile, we realize the development of novel focusing phase surfaces

Volumetric analysis of the aging auditory pathway using high resolution magnetic resonance histology

Numerous shown consequences of age-related hearing loss have been unveiled; however, the relationship of the cortical and subcortical structures of the auditory pathway with aging is not well known. Investigations into neural structure analysis remain sparse due to difficulties of doing so in animal models; however, recent technological advances have been able to achieve a resolution adequate to perform such studies even in the small mouse. We utilize 12 members of the BXD family of recombinant inbred mice and aged separate cohorts. Utilizing novel magnetic resonance histology imaging techniques, we imaged these mice and generated high spatial resolution three dimensional images which were then comprehensively labeled. We completed volumetric analysis of 12 separate regions of interest specific to the auditory pathway brainstem nuclei and cortical areas with focus on the effect of aging upon said structures. Our results showed significant interstrain variation in the age-related effect on structure volume supporting a genetic influence in this interaction. Through multivariable modeling, we observed heterogenous effects of aging between different structures. Six of the 12 regions of interests demonstrated a significant age-related effect. The auditory cortex and ventral cochlear nucleus were found to decrease in volume with age, while the medial division of the medial geniculate nucleus, lateral lemniscus and its nucleus, and the inferior colliculus increased in size with age. Additionally, no sex-based differences were noted, and we observed a negative relationship between auditory cortex volume and mouse weight. This study is one of the first to perform comprehensive magnetic resonance imaging and quantitative analysis in the mouse brain auditory pathway cytoarchitecture, offering both novel insights into the neuroanatomical basis of age-related changes in hearing as well as evidence toward a genetic influence in this interaction. High resonance magnetic resonance imaging provides a promising efficacious avenue in future mouse model hearing loss investigations

Transcriptome analysis identifies an ASD-Like phenotype in oligodendrocytes and microglia from C58/J amygdala that is dependent on sex and sociability

Abstract Background Autism Spectrum Disorder (ASD) is a group of neurodevelopmental disorders with higher incidence in males and is characterized by atypical verbal/nonverbal communication, restricted interests that can be accompanied by repetitive behavior, and disturbances in social behavior. This study investigated brain mechanisms that contribute to sociability deficits and sex differences in an ASD animal model. Methods Sociability was measured in C58/J and C57BL/6J mice using the 3-chamber social choice test. Bulk RNA-Seq and snRNA-Seq identified transcriptional changes in C58/J and C57BL/6J amygdala within which DMRseq was used to measure differentially methylated regions in amygdala. Results C58/J mice displayed divergent social strata in the 3-chamber test. Transcriptional and pathway signatures revealed immune-related biological processes differ between C58/J and C57BL/6J amygdala. Hypermethylated and hypomethylated genes were identified in C58/J versus C57BL/6J amygdala. snRNA-Seq data in C58/J amygdala identified differential transcriptional signatures within oligodendrocytes and microglia characterized by increased ASD risk gene expression and predicted impaired myelination that was dependent on sex and sociability. RNA velocity, gene regulatory network, and cell communication analysis showed diminished oligodendrocyte/microglia differentiation. Findings were verified using Bulk RNA-Seq and demonstrated oxytocin’s beneficial effects on myelin gene expression. Limitations Our findings are significant. However, limitations can be noted. The cellular mechanisms linking reduced oligodendrocyte differentiation and reduced myelination to an ASD phenotype in C58/J mice need further investigation. Additional snRNA-Seq and spatial studies would determine if effects in oligodendrocytes/microglia are unique to amygdala or if this occurs in other brain regions. Oxytocin’s effects need further examination to understand its’ potential as an ASD therapeutic. Conclusions Our work demonstrates the C58/J mouse model’s utility in evaluating the influence of sex and sociability on the transcriptome in concomitant brain regions involved in ASD. Our single-nucleus transcriptome analysis elucidates potential pathological roles of oligodendrocytes and microglia in ASD. This investigation provides details regarding regulatory features disrupted in these cell types, including transcriptional gene dysregulation, aberrant cell differentiation, altered gene regulatory networks, and changes to key pathways that promote microglia/oligodendrocyte differentiation. Our studies provide insight into interactions between genetic risk and epigenetic processes associated with divergent affiliative behavior and lack of positive sociability

ON-SKY PERFORMANCE ANALYSIS OF THE VECTOR APODIZING PHASE PLATE CORONAGRAPH ON MagAO/Clio2

We report on the performance of a vector apodizing phase plate coronagraph that operates over a wavelength range of 2-5 mu m. and is installed in MagAO/Clio2 at the 6.5 m Magellan Clay telescope at Las Campanas Observatory, Chile. The coronagraph manipulates the phase in the pupil to produce three beams yielding two coronagraphic point-spread functions (PSFs) and one faint leakage PSF. The phase pattern is imposed through the inherently achromatic geometric phase, enabled by liquid crystal technology and polarization techniques. The coronagraphic optic is manufactured using a direct-write technique for precise control of the liquid crystal pattern. and multitwist retarders for achromatization. By integrating a linear phase ramp to the coronagraphic phase pattern, two separated coronagraphic PSFs are created with a single pupil-plane optic, which makes it robust and easy to install in existing telescopes. The two coronagraphic PSFs contain a 180 degrees dark hole on each side of a star, and these complementary copies of the star are used to correct the seeing halo close to the star. To characterize the coronagraph, we collected a data set of a bright (m(L) = 0-1) nearby star with similar to 1.5 hr of observing time. By rotating and optimally scaling one PSF. and subtracting it from the other PSF, we see a contrast improvement by 1.46 magnitudes at 3.5 lambda/D. With regular angular differential imaging at 3.9 mu m, the MagAO vector apodizing phase plate coronagraph delivers a 5 sigma Delta mag contrast of 8.3 (= 10(-3.3)) at 2 lambda/D and 12.2 (= 10(-4.8)) at 3.5 lambda/D.Netherlands Organization for Scientific Research (NWO); European Research Council [678194]; NASA Exoplanets Research Program (XRP) [NNX16AD44G]This item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at [email protected]

ON-SKY PERFORMANCE ANALYSIS OF THE VECTOR APODIZING PHASE PLATE CORONAGRAPH ON MagAO/Clio2

We report on the performance of a vector apodizing phase plate coronagraph that operates over a wavelength range of $2-5 \mu$m and is installed in MagAO/Clio2 at the 6.5 m Magellan Clay telescope at Las Campanas Observatory, Chile. The coronagraph manipulates the phase in the pupil to produce three beams yielding two coronagraphic point-spread functions (PSFs) and one faint leakage PSF. The phase pattern is imposed through the inherently achromatic geometric phase, enabled by liquid crystal technology and polarization techniques. The coronagraphic optic is manufactured using a direct-write technique for precise control of the liquid crystal pattern, and multitwist retarders for achromatization. By integrating a linear phase ramp to the coronagraphic phase pattern, two separated coronagraphic PSFs are created with a single pupil-plane optic, which makes it robust and easy to install in existing telescopes. The two coronagraphic PSFs contain a 180$^\circ$ dark hole on each side of a star, and these complementary copies of the star are used to correct the seeing halo close to the star. To characterize the coronagraph, we collected a dataset of a bright ($m_L=0-1$) nearby star with $\sim$1.5 hr of observing time. By rotating and optimally scaling one PSF and subtracting it from the other PSF, we see a contrast improvement by 1.46 magnitudes at $3.5 \lambda/D$. With regular angular differential imaging at 3.9 $\mu$m, the MagAO vector apodizing phase plate coronagraph delivers a $5\sigma\ \Delta$ mag contrast of 8.3 ($=10^{-3.3}$) at 2 $\lambda/D$ and 12.2 ($=10^{-4.8}$) at $3.5 \lambda/D$.Comment: Published in ApJ. 8 figures, 1 table. Received 2016 June 17; revised 2016 November 3; accepted 2016 November 28; published 2017 January 1