Live Imaging of Microglia During Sleeping Sickness Reveals Early and Heterogeneous Inflammatory Responses

INTRODUCTION: Invasion of the central nervous system (CNS) is the most serious consequence of METHODS: To further address this issue, we implanted a cranial window on the cortex of B6.129P2(Cg)- RESULTS AND DISCUSSION: We uncovered an early involvement of microglia that precedes invasion of the CNS by the parasite. We accomplished a detailed characterization of the progressive sequence of events that correlates with microglial morphological changes and microgliosis. Our findings unveiled a heterogeneous microglial response in places of initial homeostatic disruption near brain barriers and pointed out an exceptional capability of microglia to hamper parasite proliferation inside the brain. We also found early signs of inflammation in the meninges, which synchronize with the microglial response. Moreover, we observed a massive infiltration of peripheral immune cells into the parenchyma as a signature in the final disease stage. Overall, our study provides new insights into the host-pathogen immune interactions in the meningeal and parenchymal compartments of the neocortex

The Grizzly, April 21, 1989

Spring Weekend a Whopper! • Ours Nouveau • Sunday\u27s Reception Huge Success • Letter: Shed Miniskirts for Spandex • Heritage Day • Berman Roofing Top Hat Affair • \u27Packers Hike Hick Hills • Ground Round: Super Service • Running\u27s More Than Just Winning • Spring Sports: Hot and Cold • Mr. Ursinus a Whomping Good Time!https://digitalcommons.ursinus.edu/grizzlynews/1236/thumbnail.jp

The Grizzly, May 2, 1989

Greeks Grab Spotlight • Grad. Speakers Tapped • Benedict No.1 • Letter: Racism! Not Just Rednecks • Williams\u27 Farewell • Richter Honored • ISIC\u27s for Going Abroad • The Arena: Seeking Opinions • Trenton Draws First Blood • U.C. Hits Stumbling Block • Positive for MAC\u27s • Crabs and Fries: The Spice of Life • Dance Marathon Coming • Senior Altruism Needed • Seniors\u27 Lasting Impressions: Your Most Memorable Moments at U.C.? • Final Exam Schedulehttps://digitalcommons.ursinus.edu/grizzlynews/1237/thumbnail.jp

The Grizzly, March 3, 1989

Quad Asbestos Cover-Up? • UC Lip-synching Talents Shown at Airband • Letters: Klee Clears Clutter; Fondots Tepid About Water; Students Concerned • Bond is Back! • Isaac\u27s Astounds Aussie • Men\u27s Indoor Ready for Mainstream • Youthful Bears Swim to 3rd • Senior Trio Leads Ursinus • McGowan\u27s Courage Inspiring • Bears End Season Upbeat • Send North Up The River?https://digitalcommons.ursinus.edu/grizzlynews/1231/thumbnail.jp

The Grizzly, January 27, 1989

Constructium Ursini • Ad Hoc Hoists Honor • New GPA: 2.25 or Dive! • Letter: Green Shirt Makes Reed Red • Fair Not Just for Freshmen • Security Shacks in Reimert • Beverly Oehlert Named to Pottstown Board of Directors • Final Red and Gold Day • Hoopsters Stunning in Second • Lady Bears Go For Title • U.C. Hockey Bids Boyd Bon Voyage • A \u27bears Recover from Fla. • Dryfoos, Knauer Newest Dirs. • Bailey Bandies With Bush • Grim Gripes: Wismer Hard to Swallow • Quintet Jazzes Up First Forum • Greenstein to Performhttps://digitalcommons.ursinus.edu/grizzlynews/1226/thumbnail.jp

The Grizzly, February 3, 1989

Hoc\u27sters Hammer Out Honesty Hazards • Middleton Mitts Mega-Bucks to Make Meta-Blood • Absentees Abound: Apathy Apparent • Letter: Bussers and Shirts Ream Reed • Pinsker\u27s Pace Paralyzes Prof. Pack • Grim Groans at Rock Ignorants • Plan B Plants U.C. in Place • Lady Bears Breeze by Lehigh • Hoopsters Haul Ball • AquaBears Swim On • Seniors Snarf Steak • Cinders Casting • Durst Demonstrates Decking Deftness • U.C. Supposedly Safe and Sound • Fruit Fantasy Makes Maxi Delight • U.C. Mourns Losshttps://digitalcommons.ursinus.edu/grizzlynews/1227/thumbnail.jp

The Grizzly, September 28, 1989

Berman Art Center Ready to Roll • New Forum Frontier to be Forged • Letter: Granite Windows Grosses Grad • Stern Happy at Ursinus • Bear Pack Run: Join Us! • Bush Wacked at Drug Dialog • Security New Park Patrol • Wellness Days! • Crutcher Leads Lady Bears to Win • Ursinus Nipped by WMC • Soccer: So-So • V-ball: Optimistic View • Athletes of the Week • Hallinger Competes on Wheels • Calliope Blast from Pasthttps://digitalcommons.ursinus.edu/grizzlynews/1242/thumbnail.jp

The Grizzly, March 17, 1989

Pledging Violations Abound: Change Imminent • Approaching Commencement Scares Seniors • Letters: Bermans Ship Garbage Here?; Alumni Concerned About Curtis Fire; Bond Facts Straight?; Collegeville Water Now Non-Toxic; Visser Vicous to Hocsters • CAB Earns Kudos • Bears Look to Bright Furture • Lady Bears Always A Bridesmaid • Track Stars Shine! • Easton\u27s Colonial Genuine Italian • Blues: A+ • Guess Who\u27s Coming for Dinner? • Senior Matters Leave Their Mark • Peruvian Junglehttps://digitalcommons.ursinus.edu/grizzlynews/1232/thumbnail.jp

The Grizzly, April 14, 1989

Middle States: What To Look For At U.C. • Sorority Songfest Continues • Letters: Lack of MACLAS Coverage Disturbing; I.D. Hassles; We Smell a Rat! • Middle States Closing Offers Suggestions • Canterbury Corner too Costly • Lacrosse Braces for Traditional Rivalry • Outmanned, But Still Best • Tennis Turns Tables • Ursinus Sweeps • Men\u27s Lax Gets Physical • Cinders Burnin\u27 Down the House • Guess Who\u27s Coming to Dinner? • Branker Wins Jazz Fellowship • MACLAS Meeting Absolute Success • Sternal: Functional Art at U.C. • Cyclists Rolling to Victories • Pilgrim Continues U.C. Evaluation • Admissions Reception • Grant to Give Biology a Boost • The Music Scenehttps://digitalcommons.ursinus.edu/grizzlynews/1235/thumbnail.jp

The Grizzly, April 7, 1989

History in Making: Middle States Arrive Sunday • APO Holds Local Fraternity Chapter Conference • Letters: Normal Pledging O.K.; Booby not Prize • DeLeon Woos Wismer • Hundreds to Storm Campus • Friday\u27s Fun, But Not Food Fantasy • Men\u27s Lacrosse Begins with a Bang! • Bowers Leads U.C. to Win • Driscoll, Ursinus Set High Goals • Tough Schedule to Benefit U.C. Lax • Two Week Pledging Proposed by Subcommittee on Greeks • Boston: Seats Going Fast • It\u27s Tenure Time Once Again! • U.S.G.A Update • Cinders to Rock U.C. • U.S.G.A. Needs You! • Rumors a Real Bomb • Take a Bite! • College Displays Crime Statshttps://digitalcommons.ursinus.edu/grizzlynews/1234/thumbnail.jp