Improved Recovery of Exfoliated Colonocytes from Feces Using Newly Developed Immunomagnetic Beads

We demonstrated the feasibility of a new methodology for isolating colonocytes from feces. To reduce costs and improve the recovery rate of colonocytes from feces, we attempted to develop new immunomagnetic beads. Several sizes of magnetic beads were prepared and tagged with a monoclonal antibody against EpCAM. We made several new monoclonal antibodies against EpCAM, and each monoclonal antibody was tagged to the magnetic beads. In the simulation, the most efficient recovery of HT-29 cells was obtained using the smallest size of beads. Also, beads tagged with a monoclonal antibody with a higher affinity against EpCAM had a higher recovery rate. Similar results were obtained when the smallest size of beads with the highest-affinity monoclonal antibody was applied to clinical samples. The newly developed immunomagnetic beads may be useful for isolating colorectal cancer cells from feces, enabling the cytological or molecular biological diagnosis of CRC

18. asırda "Hırkai şerif" ziyareti

Taha Toros Arşivi, Dosya No: 120-Saraylar. Not: Gazetenin "Tarihten Sahifeler" köşesinde yayımlanmıştır.İstanbul Kalkınma Ajansı (TR10/14/YEN/0033) İstanbul Development Agency (TR10/14/YEN/0033

カンゴガク OSCE ニオケル ヒョウカシャカン ノ イッチド ノ ケントウ : レイワ 4ネンド ジッシ ニヨル ヒョウカ カラ

本論文の目的は、本学看護学科において令和4年度に実施された看護学OSCE (Objective Structured Clinical Examination；以下OSCEとする）の評価について、評価者間の一致度を分析することにより、今後の看護学OSCEの評価方法に関し検討することである。本学で実施している看護学OSCEの6領域6課題について、各課題につき2名の評価者間の一致度について分析した。看護学OSCE受験者59名について、受験者1名につき試験評価者2名が別々に評価したデータを対象とした。評価者間の一致度は、設定した6課題ごとの総スコアについては級内相関係数（intra-class correlation coefficients ; 以下ICCとする）を用いて検証した。6課題の総スコアにおけるICCは0.461-0.978と中程度の一致からほぼ完全な一致が認められた。また、各課題の評価項目を内容から「基本的コミュニケーション」、「看護技術（アセスメント）」、「看護技術（適切な実施）」、「応用的実践」の4カテゴリに分類し、カテゴリごとのスコアについて評価者間の一致度をκ係数により評価した。各課題におけるカテゴリ別のκは0.348-1.000と課題の内容による差異はあるものの軽度から高い一致が認められた。今後は、評価項目について評価の一致を検討しつつ、課題の特徴を捉え課題の組み合わせ等において可能な限り工夫や検討をする必要があると考えられた。departmental bulletin pape

Simple and reliable enumeration of Escherichia coli concentrations in wastewater samples by measuring β-d-glucuronidase (GUS) activities via a microplate reader

Monitoring of Escherichia coli concentrations at wastewater treatment plants (WWTPs) is important to ensure process performance and protect public health. However, conventional E. coli enumeration methods are complicated and time- and labor-consuming. Here, we report a novel simple and reliable method based on β-D-glucuronidase (GUS) activity assay to enumerate E. coli concentrations in wastewater (WW) samples. An aliquot (20 μL) of the medium with fluorogenic enzyme substrate for E. coli and 180 μL of a WW sample were added to one well of a 96-well microplate. The microplate was placed in a microplate reader at 37°C. To this end, the fluorescence intensity of a fluorogenic enzyme substrate for E. coli was measured every 10 min over 3 h to determine GUS activity. The linear increase in the fluorescence intensity representing the GUS activities showed a positive correlation with E. coli concentrations in wastewater samples. However, the correlation equations were specific to WWTPs, which could be due to the difference in the E. coli population structures among WWTPs. We observed that the wastewater matrix is not a limitation to measure the GUS activity, and a WWTP-specific correlation equation can be used as a calibration curve to estimate the E. coli concentrations in the samples collected from that site. A comparison of the results with those of culture-dependent Colilert method proved that the current method is simple and useful for the enumeration of E. coli concentrations in wastewater samples reliably