Radioimmunoassay of Plasma 17α-Hydroxyprogesterone

A radioimmunoassay for the measurement of 17α-hydroxyprogesterone (17α-OHP) in plasma was investigated utilizing antiserum produced by the introduction of 17α-hydroxyproges- terone-3-oxim BSA. Accuracy was such that within-assay variance was 16.2% and between-assay variance 18.3%. The 17α-OHP mean plasma levels were 930±201 pg/ml(n=8)for normal adult males and 402±186 pg/ml(n=11)in the follicular phase and 1,190±662 pg/ml(n=12)in the luteal phase of females with a normal menstrual cycle. The 17α-OHP levels in some tissues and tissue fluid also were measured, being 18.06±12.91 ng/ml(n=6) in the normal trophoblastic tissue, 1.7±0.5 ng/ml(n=17) in the trophoblastic tissue of a hydatidiform mole and 855.5±507.4 ng/ml(n=5)in lutein cyst fluid

Radioimmunoassay of Estradiolusing Estradiol-6-oxim-BSA Anti Serum

A radioimmunoassay (RIA) of estradiol was performed with the use of antiserum produced by estradiol-6-oxim-BSA. The precision of the assay was such as to result in a within-assay variance of 10.1% and a between-assay variance of 31.8%. The concentrations of estradiol thus determined in the serum of women with normal menstrual cycles were 69.0±40.6 pg/ml inthe menstrual phase, 127.8±70.3 pg/ml in the follicular phase, 281.9±113.5 pg/ml in the ovulatory phase, and 165.4±79.5 pg/ml in the luteal phase. The blood estradiol level durinh pregnancy ranged from 0.28 to 13.17 ng/ml in the first trimester, 2.6 to 48.1 ng/ml in the second trimester, and 13.5 to 130.0 nh/ml in the third trimester