Genomic organization, expression analysis, and chromosomal localization of the mouse PEX3 gene encoding a peroxisomal assembly protein

The peroxin Pex3p has been identified as an integral peroxisomal membrane protein in yeast where pex3 mutants lack peroxisomal remnant structures. Although not proven in higher organisms, a role of this gene in the early peroxisome biogenesis is suggested, We report here the cDNA cloning and the genomic structure of the mouse PEX3 gene. The 2 kb cDNA encodes a polypeptide of 372 amino acids (42 kDa). The gene spans a region of 30 kb, contains 12 exons and 11 introns and is located on band A of chromosome 10, The putative promoter region exhibits characteristic housekeeping features. PEX3 expression was identified in all tissues analyzed, with the strongest signals in liver and in testis, and could not be induced by fenofibrate. The data presented may be useful for the generation of a mouse model defective in PEX3 in order to clarify the yet unknown functional impact of disturbances in early peroxisomal membrane assembly

Study of retention of organophosphorus compoundsand volatile organic solventson polydimethylsiloxane using inverse gas chromatography

A detailed study of retention of the variety of volatile organic solvents (VOS) and organophosphorus compounds on the polydimethylsiloxane (PDMS) preliminary characterized by FTIR and DSC methods was carried out by means of an inverse gas chromatography technique. The specific retention volumes and infinite dilution activity coefficients were determined for different classes of VOCs, linear and cyclic alkanes, chloroalkanes, oxygen compounds (alcohols, ketones, and esters), and nitriles as well as for typical organophosphorus compounds, 4-nitrophenyl esters of diethyl phosphoric (paraoxon) and diethyl thiophosphoric (parathion) acids. The effect of dipole-dipole interactions and dispersion interactions on the efficacy of the retention of the studied compounds on the PDMS was analyzed. Organophosphorus stimulants are characterized by strong retention on the PDMS, the difference in their retention are to be connected with more efficient dipole-dipole interaction of the oxygen atom of paraoxon with the PDMS than that of the sulphur atom of parathion. A possibility of using PDMS as a base for the detection layers sensitive to organophosphorus compounds was demonstra