Les peptides antimicrobiens d’origine microbienne: cas des bactériocines

Les bactéries à Gram positif et à Gram négatif et les archées produisent respectivement des bactériocines et des archéocines. Les bactériocines et les archéocines sont des peptides antimicrobiens. Au fil des ans leur rôle dans la sûreté des produits alimentaires n’a cessé d’être démontré, faisant de ces peptides naturels un centre d’intérêt pour la recherche scientifique. Cette revue tente de faire l’état des connaissances sur les bactériocines produites par les bactéries Gram positif, bactéries Gram négatif et les archées en faisant ressortir leurs organisations génétiques particulières, leurs modes d’action qui diffèrent de celui des antibiotiques et surtout leurs larges spectres d’action. La collecte des données a consisté à consulter les articles scientifiques publiés et les mémoires de thèses sur les différents travaux menés sur les bactériocines et disponible en ligne via les journaux de publication et les sites dédiés aux collectes de thèses en ligne. Ces peptides antimicrobiens pourraient constituer une alternative pour la conservation des denrées alimentaires surtout en milieu rural dans les pays en voie de développement.© 2016 International Formulae Group. All rights reserved.Mots clés: bactériocines, bactéries, archéocines, archées, antimicrobiens, agro alimentaireEnglish Title: Antimicrobial peptides from microbes: case of bacteriocinsEnglish AbstractGram-positive and Gram-negative bacteria and Archaea respectively produce bacteriocins and archeocins. Over the years, their role in the food safety has continued rising because of the report of many works. This review focuses on bacteriocins know up to date by highlighting on their particular genetic organization, their mode of action that differs from antibiotics and especially their broad spectrum of action. Data were collected by consulting online available published scientific articles and thesis on bacteriocins and archeocins. These antimicrobial peptides could provide an alternative for preserving foodstuffs especially in rural areas in developing countries.© 2016 International Formulae Group. All rights reserved.Keywords: Bacteriocins; bacteria; archeocins, archea, antimicrobial activity, agribusines

Sida acuta Burm. f.: a medicinal plant with numerous potencies

Sida acuta is shrub belonging to Malvaceae family. The plant is widely distributed in the subtropical regions where it is found in bushes, in farms and around habitations. Surveys conducted in indigenous places revealed that the plant had many traditional usages that varied from one region to another. The most cited illnesses are fever, headache and infections diseases. Indeed, many laboratory screening have been conducted to show the scientific rationale behind these usages and many compounds havebeen isolated from the plant. In the present review we listed the plant usages in folk medicine in some regions where the plant grows and we discussed on the confirmed in vitro activities after laboratoryscreenings. The review ended with the pharmacological properties of several compounds isolated from S. acuta principally alkaloids

Antioxidant and antibacterial activities of polyphenols from ethnomedicinal plants of Burkina Faso

Polyphenols from four medicinal plants of Burkina Faso, Combretum micranthum, Khaya senegalensis, Pterocarpus erinaceus and Sida acuta, were screened for their antioxidant and antimicrobial activities against pathogenic bacteria. The medicinal plants displayed different polyphenols contents and antioxidant activities. The bark of P. erinaceus had the highest antioxidant activity. Some microorganisms were susceptible to polyphenol extracts with minimal bactericidal concentration values between 20 and 2000 mg/ml while other microorganisms appeared to be resistant to the extracts. Microbicide and microbiostatic activities of the extracts were dependent on the type of strains. Results suggest that these plants are not only interesting sources for antimicrobial activities but also potential sources of phenolic antioxidants

Ethnobotanical survey of medicinal plants used in the management of hypertension in the Maritime region of Togo

Background: Hypertension is one of the most common chronic diseases in modern societies and there is evidence that its incidence and severity are increasing. This survey was conducted to investigate the plants used by the Togolese traditional healers to treat the disease.Method: From January to June 2016, an ethnobotanical survey was conducted using a semi-structured questionnaire with traditional healers (TH) in the southern region of Togo. The importance of the plants was assessed by the calculation of the use value (UV).Results: In Total, 128 TH male and female were interviewed and 116 plants species belonging to 46 families were identified as treating hypertension. The most represented families were: Fabaceae with 16 species followed by Euphorbiaceae and Rutaceae contributing with 8 and 6 species respectively. Based on the calculated use values the most important species were Byrsocarpus coccineus Schum. Et thonn. (UV = 0.47); Crateva religiosae G.Forst. (UV = 0.47), Boerhavia diffusa Engelm. &A.Gray L. (UV = 0.47), Xylopia athiopica A. Rich. (UV = 0.42), Mangnifera indica L. (UV = 0.38). The leaves and the roots were the parts of plant predominantly used to prepare the recipes, mainly decoctions administrated by oral route. Clinical manifestations such as dizzy spells, swarming, loss of consciousness, severe headache, severe anxiety and shortness of breath, nosebleed, and fear of heights were used by TH to diagnose the disease.Conclusion: This study showed initial evidence of the use of plant materials by Togolese TH to treat hypertension. These results constitute a database for pharmacological screenings with the aim of developing new therapies.Key words: Hypertension, traditional medicine, ethnobotanical survey, medicinal plants, Togo

ETHNOBOTANICAL STUDY OF PLANTS USED TO TREAT ASTHMA IN THE MARITIME REGION IN TOGO

Background: Asthma is one of the most common chronic diseases in modern society and it is evident that its incidence and severity are increasing, however very little is known about the plants used in the management of the disease. This study therefore aimed to document the plants usage in the Togolese traditional medicine to treat asthma. Methodology: From January to June 2015, an ethnobotanical survey was conducted using a semi-structured questionnaire with traditional healers (THs) in the southern region of Togo. The importance of the plants species was assessed by the calculated use values. Results: In Total, 121 THs (92 males and 29 females) were interviewed and 98 plants species belonging to 54 families were identified as curing asthma. The most represented families were: Leguminosae with 7 species followed by Euphorbiaceae and Rutaceae contributing with 6 and 5 species respectively. Based on the calculated use values the most important species were Carcica papaya L., Cataranthus roseus L., Eucalyptus camaldulensis Dehnh., Piper guineense Thonn., Eucalyptus citriodora Hook., Eucalyptus globules Labill. and Euphorbia hirta L. The leaves and the root were the parts predominantly used to prepare the formulations, mainly decoctions, administrated by oral route. Clinical manifestations such as wheezing (91.74%), difficulty as speaking or coughing (73.55%), dyspnea (66.94%), dry cough (52.89%), sweating and increased heart rate (52.07%) were used by TH to diagnose the disease. Conclusion: This study showed initial evidence of the use of plant materials by Togolese TH to heal asthma. These results could be a starting point for laboratory screenings

Antibacterial activity of alkaloids from Sida acuta

Sida acuta is a shrub indigenous to pantropical regions. The plant is widely used for its various pharmacological properties. Among compounds of pharmacological interest occurring in the plant, are indoloquinoline alkaloids. The aim of the present study was to investigate the antimicrobial activity ofalkaloids of S. acuta from Burkina Faso. The alkaloids had a good antimicrobial activity against the test microorganisms. In the agar-well diffusion assay, highest inhibition zone diameters were recorded with Gram-positive bacteria. The broth microdilution assay gave minimal inhibitory concentration values ranging from 16 to 400 ìg/ml and minimal bactericidal concentration values ranging from 80 to up to 400 ìg/ml. The gas chromatography-mass spectrometry analysis of the same alkaloids led to the identification of cryptolepine and quindoline as the major components

Antibacterial and anti-inflammatory activities of crude extracts of three Togolese medicinal plants against ESBL Klebsiella pneumoniae strains

Background: Pithecellobium dulce (Roxb.) Benth., Securidaca longepedunculata Fresn and Cryptolepis sanguinolenta (Lindl.) Schlt are three plants widely used in the Togolese traditional medicine to treat microbial infections. Some studies reported their antibacterial activity alone but until know there no data concerning their possible interaction with conventional antibiotics. The main objective of this study was to investigate the antibacterial activity of the association of the crude extracts of the three plants with some conventional antibiotics. We further evaluate the antioxidant and the anti-inflammatory activities of the extracts on rat’s model.Materials and methods:The antimicrobial activity was evaluated by the broth microdilution assay and the Fractional inhibitory concentrations (FIC) determined by the checkerboard method. The anti-inflammatory activity was evaluated using the Carrageenan- induced rat paw edema model. The antioxidant activities and the phenol contents were determined by spectrophotometry.Results: The MICs of hydroethanolic extract of plants ranged from 3.125 to 100 mg/mL on Klebsiella pneumoniae strains. Synergistic action was observed only with the combination of Imipenem/P. dulce, imipenem/C. sanguinolenta, amikacin/P. dulce and amikacin/C. sanguinolenta against the ESBL negative Klebsiella pneumoniae strain. Of the 21 associations, 15 were antagonistic on the ESBL-producing strains. The indifference effect was observed with the combination of the extract of Securidaca longepedunculata and the following antibiotics imipenem, amikacin, tetracyclin, ciprofloxacin, Cefotaxim; and Sulfametoxazol+Trimethoprim. The in vitro anti-inflammatory with Lipoxygenase inhibition activity was best with C. sanguinolenta extract while the in vivo paw edema model revealed that S. longepedunculata was the highest reducer of paw edema. In addition white blood cells count and biochemical parameters such as total proteins and immunoglobulins were significantly affected by the administration of plant extracts.Conclusion: This study revealed that the three plants although they may inhibit the bacterial growth by themselves, but there is also a possible synergistic action with the commercial antibiotics. Further investigations are needed to identify the active compounds and their mechanism of action.Keywords: antibiotics, extended-spectrum beta lactamase, plant extract, bacteria, Tog

ETHNOBOTANICAL SURVEY OF MEDICINAL PLANTS USED IN THE MANAGEMENT OF HYPERTENSION IN THE MARITIME REGION OF TOGO

Background: Hypertension is one of the most common chronic diseases in modern societies and there is evidence that its incidence and severity are increasing. This survey was conducted to investigate the plants used by the Togolese traditional healers to treat the disease. Method: From January to June 2016, an ethnobotanical survey was conducted using a semi-structured questionnaire with traditional healers (TH) in the southern region of Togo. The importance of the plants was assessed by the calculation of the use value (UV). Results: In Total, 128 TH male and female were interviewed and 116 plants species belonging to 46 families were identified as treating hypertension. The most represented families were: Fabaceae with 16 species followed by Euphorbiaceae and Rutaceae contributing with 8 and 6 species respectively. Based on the calculated use values the most important species were Byrsocarpus coccineus Schum. Et thonn. (UV = 0.47); Crateva religiosa G.Forst. (UV = 0.47), Boerhavia diffusa Engelm. &A.Gray L. (UV = 0.47), Xylopia aethiopica A. Rich. (UV = 0.42), Mangnifera indica L. (UV = 0.38). The leaves and the roots were the parts of plant predominantly used to prepare the recipes, mainly decoctions administrated by oral route. Clinical manifestations such as dizzy spells, swarming, loss of consciousness, severe headache, severe anxiety and shortness of breath, nosebleed, and fear of heights were used by TH to diagnose the disease. Conclusion: This study showed initial evidence of the use of plant materials by Togolese TH to treat hypertension. These results constitute a database for pharmacological screenings with the aim of developing new therapies

Revue sur l’Ail et ses Composés Bioactifs

Introduction: Dans les pays en voie de développement, les problèmes du médicament se posent en termes d'insuffisance quantitative, qualitative et d'inaccessibilité économique. Pour pallier à ce problème, près de 80% de la population ont recours à la médecine traditionnelle. Cependant, la question n'est plus de démontrer l'efficacité de la médecine traditionnelle mais de s'en convaincre et convaincre les acteurs de la santé publique et des groupes de promotion de la santé. D’où notre objectif de vulgariser l’utilisation de l’ail par la population en montrant les composés bioactifs et les differentes formes d’ail et leur usage thérapeutique. Méthodologie: la recherche Littérature a été effectuée dans les bases de donnée de Medline, Google scholar, Research Gate, HINARI, Cochrane et livres en utilisant des mots clés tels que : Problématique des médicaments en Afrique, l’étude ethnobotanique, ethnomédecine, ethnopharmacologie, les composés bioactifs de l’ail. Cette recherche documentaire s’est faite sur la période du 08 juin 2018 au 23 Novembre 2018. La recherche a été étendue sur les formes d’ail, les données cliniques de l’utilisation de l’ail. Synthèse: L’ail est utilisé depuis des millénaires tant pour ses talents culinaires que ses propriétés thérapeutiques. Les principaux composés responsables des vertus thérapeutiques sont les composés organosulfureux dont leur présence et concentration dépend de la forme d’ail utilisé. Conclusion: L’ail, permet de lutter contre certains germes infectieux de la peau et contre les parasites. Il est aussi utilisé dans le traitement de l’hypertension artérielle, de la coagulation du sang et l’hypercholestérolémie. Ainsi, il est recommandé de consommer des préparations conditionnées et standardisées de l’ail pour bénéficier de ses vertus.Introduction: The availability and accessibility of medicines remains a real concern in developing countries. In the context of low economic income, most of the population (80%) uses traditional medicine. The effectiveness of traditional medicine has been well established. This study suggests that public health actors and health promotion groups in developing countries use herbal medicine as an alternative to the problem of the effectiveness of chemical synthesis drugs. This paper focuses on evaluating the use of different forms of garlic by the population and the bioactive compounds of this plant. Methodology: A literature review was conducted in the Medline, Google Scholar, Research Gate, HINARI, and Cochrane databases using keywords such as Drugs in Africa, Ethnobotany, Ethnomedicine, Ethnopharmacology, Garlic Forms, and Compounds bioactives of garlic. Results: The use of garlic is as old as humanity especially in cooking and disease therapy. The main compounds responsible for the therapeutic virtues are the organosulfur compounds, and its concentration of which depends on the shape of the garlic. Conclusion: Garlic can fight against skin infections, blood pressure drop, prevent blood clotting and hypercholesterolemia. Based on this study, it is recommended to consume conditioned and standardized garlic preparations in order to benefit from its virtues