Radiation-induced changes in serum lipidome of head and neck cancer patients

Cancer radiotherapy (RT) induces response of the whole patient’s body that could be detected at the blood level. We aimed to identify changes induced in serum lipidome during RT and characterize their association with doses and volumes of irradiated tissue. Sixty-six patients treated with conformal RT because of head and neck cancer were enrolled in the study. Blood samples were collected before, during and about one month after the end of RT. Lipid extracts were analyzed using MALDI-oa-ToF mass spectrometry in positive ionization mode. The major changes were observed when pre-treatment and within-treatment samples were compared. Levels of several identified phosphatidylcholines, including (PC34), (PC36) and (PC38) variants, and lysophosphatidylcholines, including (LPC16) and (LPC18) variants, were first significantly decreased and then increased in post-treatment samples. Intensities of changes were correlated with doses of radiation received by patients. Of note, such correlations were more frequent when low-to-medium doses of radiation delivered during conformal RT to large volumes of normal tissues were analyzed. Additionally, some radiation-induced changes in serum lipidome were associated with toxicity of the treatment. Obtained results indicated the involvement of choline-related signaling and potential biological importance of exposure to clinically low/medium doses of radiation in patient’s body response to radiation

Systemic Effects of Radiotherapy and Concurrent Chemo-Radiotherapy in Head and Neck Cancer Patients—Comparison of Serum Metabolome Profiles

Anticancer treatment induces systemic molecular changes that could be detected at the level of biofluids. Understanding how human metabolism is influenced by these treatments is crucial to predict the individual response and adjust personalized therapies. Here, we aimed to compare profiles of metabolites in serum of head and neck cancer patients treated with concurrent chemo-radiotherapy, radiotherapy alone, or induction chemotherapy. Serum samples were analyzed by a targeted quantitative approach using combined direct flow injection and liquid chromatography coupled to tandem mass spectrometry, which allowed simultaneous quantification of 149 metabolites. There were 45 metabolites whose levels were significantly changed between pretreatment and within- or post-treatment serum samples, including 38 phospholipids. Concurrent chemo-radiotherapy induced faster and stronger effects than radiotherapy alone. On the other hand, chemotherapy alone did not result in significant changes. The decreased level of total phospholipids was the most apparent effect observed during the first step of the treatment. This corresponded to the loss of patients’ body mass, yet no correlation between both parameters was observed for individual patients. We concluded that different molecular changes were measured at the level of serum metabolome in response to different treatment modalities

Measuring Radial Variation in Basic Density of Pendulate Oak: Comparing Increment Core Samples with the IML Power Drill

To determine the appropriate final use of wood from a particular tree species, it is first necessary to know its properties. Methods that use wood samples taken directly from the trunk of a growing or felled tree are very time-consuming and require a great deal of manual work. Non-destructive methods may be more effective and much faster, thanks to the use of advanced technologies. The resistance drilling can be used to determine the variation in wood density along the radius of the stem. The main aim of the present study was to determine the basic density of the wood on a cross-section of the trunk of oak trees and to investigate the correlation of the results with those obtained by drilling the same trees with the IML RESI-PD 400. The results of Spearman’s correlation test showed strong positive correlations between all examined properties. We observed a trend whereby the density of the wood and Resi amplitude increased in direct proportion to the width of the annual rings. The results of linear regression show a strong relationship between examined properties. This study provide evidence that the Resi is an appropriate tool for non-destructive determination of wood density

The Metabolic Footprint of Systemic Effects in the Blood Caused by Radiotherapy and Inflammatory Conditions: A Systematic Review

Response to radiotherapy (RT) includes tissue toxicity, which may involve inflammatory reactions. We aimed to compare changes in metabolic patterns induced at the systemic level by radiation and inflammation itself. Patients treated with RT due to head and neck cancer and patients with inflammation-related diseases located in the corresponding anatomical regions were selected. PubMed and Web of Science databases were searched from 1 January 2000 to 10 August 2023. Twenty-five relevant studies where serum/plasma metabolic profiles were analyzed using different metabolomics approaches were identified. The studies showed different metabolic patterns of acute and chronic inflammatory diseases, yet changes in metabolites linked to the urea cycle and metabolism of arginine and proline were common features of both conditions. Although the reviewed reports showed only a few specific metabolites common for early RT response and inflammatory diseases, partly due to differences in metabolomics approaches, several common metabolic pathways linked to metabolites affected by radiation and inflammation were revealed. They included pathways involved in energy metabolism (e.g., metabolism of ketone bodies, mitochondrial electron transport chain, Warburg effect, citric acid cycle, urea cycle) and metabolism of certain amino acids (Arg, Pro, Gly, Ser, Met, Ala, Glu) and lipids (glycerolipids, branched-chain fatty acids). However, metabolites common for RT and inflammation-related diseases could show opposite patterns of changes. This could be exemplified by the lysophosphatidylcholine to phosphatidylcholine ratio (LPC/PC) that increased during chronic inflammation and decreased during the early phase of response to RT. One should be aware of dynamic metabolic changes during different phases of response to radiation, which involve increased levels of LPC in later phases. Hence, metabolomics studies that would address molecular features of both types of biological responses using comparable analytical and clinical approaches are needed to unravel the complexities of these phenomena, ultimately contributing to a deeper understanding of their impact on biological systems

The Effect of Beech (<i>Fagus sylvatica</i> L.) Bark Stripping by Deer on Depreciation of Wood

The aim of the study was to analyse the changes in the infection rate development inside the beech stem as a result of browsing by deer (Cervus elaphus). The research materials were collected from three research plots located in the Polanów Forest Inspectorate from March to April 2020. For the study, 80 beech trees were selected, for which the size of the fallow tree, the percentage of the section taken from its centre infected with rot, and the number of years passed since the tree was wounded were determined. The study shows that the infection affects only the rings formed before the tree was injured. The average size of stem rot was 7.75% of its area, and it spread at the rate of 2.52% of the cross-sectional area per year. The analysis of the obtained results proved that both the size of the wound (splits) and the time elapsed since the tree was damaged are significantly correlated with each other. It is also possible to build a model for estimating the size of decay in stunted beech trees based on easy-to-determine predictors, such as maximum wound width and elapsed time since tree damage

The Effect of Beech (Fagus sylvatica L.) Bark Stripping by Deer on Depreciation of Wood

The aim of the study was to analyse the changes in the infection rate development inside the beech stem as a result of browsing by deer (Cervus elaphus). The research materials were collected from three research plots located in the Polan&oacute;w Forest Inspectorate from March to April 2020. For the study, 80 beech trees were selected, for which the size of the fallow tree, the percentage of the section taken from its centre infected with rot, and the number of years passed since the tree was wounded were determined. The study shows that the infection affects only the rings formed before the tree was injured. The average size of stem rot was 7.75% of its area, and it spread at the rate of 2.52% of the cross-sectional area per year. The analysis of the obtained results proved that both the size of the wound (splits) and the time elapsed since the tree was damaged are significantly correlated with each other. It is also possible to build a model for estimating the size of decay in stunted beech trees based on easy-to-determine predictors, such as maximum wound width and elapsed time since tree damage

Within-Stem Differences in Moisture Content Loss during Transpiration and Air-Drying of Felled Oak Trees

This study evaluated within-stem differences in the moisture content of stored summer-harvested oak wood with respect to drying method. The felled oaks were naturally dried for eight weeks, from 4 July to 29 August 2017. We analyzed two methods of preparation and storage: a transpiration drying method (W), and an air-drying method for stem-wood (L). Transpiration drying is a better method for oak stems than air-drying. Statistically significant differences between drying methods were found after six weeks of storage. This coincided with complete wilting of the leaves. However, signs of wilting and leaf color change appeared earlier, between the second and fourth weeks of storage. In parallel, in scenario W, a statistically significant difference in MC of sapwood was observed between the second and fourth weeks of storage. Such a phenomenon was not observed in scenario L. The MC of heartwood also gradually decreased, especially in scenario W. Explanation of this phenomenon lay outside the scope of this study; however, it indicates how the structure and properties of wood, in addition to storage conditions and methods, influence the efficiency of biomass drying in the harvesting area

Cardiac endothelial cells isolated from mouse heart - a novel model for radiobiology

Cardiovascular disease is recognized as an important clinical problem in radiotherapy and radiation protection. However, only few radiobiological models relevant for assessment of cardiotoxic effects of ionizing radiation are available. Here we describe the isolation of mouse primary cardiac endothelial cells, a possible target for cardiotoxic effects of radiation. Cells isolated from hearts of juvenile mice were cultured and irradiated in vitro. In addition, cells isolated from hearts of locally irradiated adult animals (up to 6 days after irradiation) were tested. A dose-dependent formation of histone γH2A.X foci was observed after in vitro irradiation of cultured cells. However, such cells were resistant to radiation-induced apoptosis. Increased levels of actin stress fibres were observed in the cytoplasm of cardiac endothelial cells irradiated in vitro or isolated from irradiated animals. A high dose of 16 Gy did not increase permeability to Dextran in monolayers formed by endothelial cells. Up-regulated expression of Vcam1, Sele and Hsp70i genes was detected after irradiation in vitro and in cells isolated few days after irradiation in vivo. The increased level of actin stress fibres and enhanced expression of stress-response genes in irradiated endothelial cells are potentially involved in cardiotoxic effects of ionizing radiation