Noninfectious retrovirus particles drive the APOBEC3/Rfv3 dependent neutralizing antibody response.

Members of the APOBEC3 family of deoxycytidine deaminases counteract a broad range of retroviruses in vitro through an indirect mechanism that requires virion incorporation and inhibition of reverse transcription and/or hypermutation of minus strand transcripts in the next target cell. The selective advantage to the host of this indirect restriction mechanism remains unclear, but valuable insights may be gained by studying APOBEC3 function in vivo. Apobec3 was previously shown to encode Rfv3, a classical resistance gene that controls the recovery of mice from pathogenic Friend retrovirus (FV) infection by promoting a more potent neutralizing antibody (NAb) response. The underlying mechanism does not involve a direct effect of Apobec3 on B cell function. Here we show that while Apobec3 decreased titers of infectious virus during acute FV infection, plasma viral RNA loads were maintained, indicating substantial release of noninfectious particles in vivo. The lack of plasma virion infectivity was associated with a significant post-entry block during early reverse transcription rather than G-to-A hypermutation. The Apobec3-dependent NAb response correlated with IgG binding titers against native, but not detergent-lysed virions. These findings indicate that innate Apobec3 restriction promotes NAb responses by maintaining high concentrations of virions with native B cell epitopes, but in the context of low virion infectivity. Finally, Apobec3 restriction was found to be saturable in vivo, since increasing FV inoculum doses resulted in decreased Apobec3 inhibition. By analogy, maximizing the release of noninfectious particles by modulating APOBEC3 expression may improve humoral immunity against pathogenic human retroviral infections

ZFIRE: The Evolution of the Stellar Mass Tully-Fisher Relation to Redshift 2.0 < Z < 2.5 with MOSFIRE

Using observations made with MOSFIRE on Keck I as part of the ZFIRE survey, we present the stellar mass Tully-Fisher relation at 2.0 < z < 2.5. The sample was drawn from a stellar mass limited, Ks-band selected catalog from ZFOURGE over the CANDELS area in the COSMOS field. We model the shear of the Halpha emission line to derive rotational velocities at 2.2X the scale radius of an exponential disk (V2.2). We correct for the blurring effect of a two-dimensional PSF and the fact that the MOSFIRE PSF is better approximated by a Moffat than a Gaussian, which is more typically assumed for natural seeing. We find for the Tully-Fisher relation at 2.0 < z < 2.5 that logV2.2 =(2.18 +/- 0.051)+(0.193 +/- 0.108)(logM/Msun - 10) and infer an evolution of the zeropoint of Delta M/Msun = -0.25 +/- 0.16 dex or Delta M/Msun = -0.39 +/- 0.21 dex compared to z = 0 when adopting a fixed slope of 0.29 or 1/4.5, respectively. We also derive the alternative kinematic estimator S0.5, with a best-fit relation logS0.5 =(2.06 +/- 0.032)+(0.211 +/- 0.086)(logM/Msun - 10), and infer an evolution of Delta M/Msun= -0.45 +/- 0.13 dex compared to z < 1.2 if we adopt a fixed slope. We investigate and review various systematics, ranging from PSF effects, projection effects, systematics related to stellar mass derivation, selection biases and slope. We find that discrepancies between the various literature values are reduced when taking these into account. Our observations correspond well with the gradual evolution predicted by semi-analytic models.Comment: 21 pages, 14 figures, 1 appendix. Accepted for publication by Apj, February 28, 201

ZFOURGE: Using Composite Spectral Energy Distributions to Characterize Galaxy Populations at 1<z<4

We investigate the properties of galaxies as they shut off star formation over the 4 billion years surrounding peak cosmic star formation. To do this we categorize $\sim7000$ galaxies from $1<z<4$ into $90$ groups based on the shape of their spectral energy distributions (SEDs) and build composite SEDs with $R\sim 50$ resolution. These composite SEDs show a variety of spectral shapes and also show trends in parameters such as color, mass, star formation rate, and emission line equivalent width. Using emission line equivalent widths and strength of the 4000\AA\ break, $D(4000)$, we categorize the composite SEDs into five classes: extreme emission line, star-forming, transitioning, post-starburst, and quiescent galaxies. The transitioning population of galaxies show modest H$\alpha$ emission ($EW_{\rm REST}\sim40$\AA) compared to more typical star-forming composite SEDs at $\log_{10}(M/M_\odot)\sim10.5$ ($EW_{\rm REST}\sim80$\AA). Together with their smaller sizes (3 kpc vs. 4 kpc) and higher S\'ersic indices (2.7 vs. 1.5), this indicates that morphological changes initiate before the cessation of star formation. The transitional group shows a strong increase of over one dex in number density from $z\sim3$ to $z\sim1$, similar to the growth in the quiescent population, while post-starburst galaxies become rarer at $z\lesssim1.5$. We calculate average quenching timescales of 1.6 Gyr at $z\sim1.5$ and 0.9 Gyr at $z\sim2.5$ and conclude that a fast quenching mechanism producing post-starbursts dominated the quenching of galaxies at early times, while a slower process has become more common since $z\sim2$.Comment: Accepted for publication in The Astrophysical Journa

Magnetic and axial vector form factors as probes of orbital angular momentum in the proton

We have recently examined the static properties of the baryon octet (magnetic moments and axial vector coupling constants) in a generalized quark model in which the angular momentum of a polarized nucleon is partly spin $\langle S_z \rangle$ and partly orbital $\langle L_z \rangle$. The orbital momentum was represented by the rotation of a flux-tube connecting the three constituent quarks. The best fit is obtained with $\langle S_z \rangle = 0.08\pm 0.15$, $\langle L_z \rangle = 0.42\pm 0.14$. We now consider the consequences of this idea for the $q^2$-dependence of the magnetic and axial vector form factors. It is found that the isovector magnetic form factor $G_M^{\mathrm{isovec}}(q^2)$ differs in shape from the axial form factor $F_A(q^2)$ by an amount that depends on the spatial distribution of orbital angular momentum. The model of a rigidly rotating flux-tube leads to a relation between the magnetic, axial vector and matter radii, $\langle r^2 \rangle_{\mathrm{mag}} = f_{\mathrm{spin}} \langle r^2 \rangle_{\mathrm{axial}} + \frac{5}{2} f_{\mathrm{orb}} \langle r^2 \rangle_{\mathrm{matt}}$, where $f_{\mathrm{orb}}/ f_{\mathrm{spin}} = \frac{1}{3}\langle L_z \rangle / G_A$, $f_{\mathrm{spin}} + f_{\mathrm{orb}} = 1$. The shape of $F_A(q^2)$ is found to be close to a dipole with $M_A = 0.92\pm 0.06$ GeV.Comment: 18 pages, 5 ps-figures, uses RevTe

Beyond UVJ: Color Selection of Galaxies in the JWST Era

We present a new rest-frame color-color selection method using "synthetic $u_s-g_s$ and $g_s-i_s$'', $(ugi)_s$ colors to identify star-forming and quiescent galaxies. Our method is similar to the widely-used $U-V$ versus $V-J$ ($UVJ$) diagram. However, $UVJ$ suffers known systematics. Spectroscopic campaigns have shown that $UVJ$-selected quiescent samples at $z \gtrsim 3$ include $\sim 10-30\%$ contamination from galaxies with dust-obscured star formation and strong emission lines. Moreover, at $z>3$, $UVJ$ colors are extrapolated because the rest-frame J-band shifts beyond the coverage of the deepest bandpasses at $< 5~\mu m$ (typically $Spitzer$/IRAC 4.5 $\mu m$ or future $JWST$/NIRCam observations). We demonstrate that $(ugi)_s$ offers improvements to $UVJ$ at $z>3$, and can be applied to galaxies in the $JWST$ era. We apply $(ugi)_s$ selection to galaxies at $0.5<z<6$ from the (observed) 3D-HST and UltraVISTA catalogs, and to the (simulated) JAGUAR catalogs. We show that extrapolation can affect $(V-J)_0$ color by up to 1 magnitude, but changes $(u_s-i_s)_0$ color by $\leq$ 0.2 mag, even at $z\simeq 6$. While $(ugi)_s$-selected quiescent samples are comparable to $UVJ$ in completeness (both achieve $\sim$85-90% at $z=3-3.5$), $(ugi)_s$ reduces contamination in quiescent samples by nearly a factor of two, from $\simeq$35% to $\simeq$17% at $z=3$, and from $\simeq$60% to $\simeq$33% at $z=6$. This leads to improvements in the true-to-false-positive ratio (TP/FP), where we find TP/FP $\gtrsim$ 2.2 for $(ugi)_s$ at $z \simeq 3.5 - 6$, compared to TP/FP $<$ 1 for $UVJ$-selected samples. This indicates that contaminants will outnumber true quiescent galaxies in $UVJ$ at these redshifts, while $(ugi)_s$ will provide higher-fidelity samples.Comment: Submitted to Ap

The distribution of satellites around massive galaxies at 1<z<3 in ZFOURGE/CANDELS: dependence on star formation activity

We study the statistical distribution of satellites around star-forming and quiescent central galaxies at 1<z<3 using imaging from the FourStar Galaxy Evolution Survey (ZFOURGE) and the Cosmic Assembly Near-IR Deep Extragalactic Legacy Survey (CANDELS). The deep near-IR data select satellites down to $\log(M/M_\odot)>9$ at z<3. The radial satellite distribution around centrals is consistent with a projected NFW profile. Massive quiescent centrals, $\log(M/M_\odot)>10.78$, have $\sim$2 times the number of satellites compared to star-forming centrals with a significance of 2.7$\sigma$ even after accounting for differences in the centrals' stellar-mass distributions. We find no statistical difference in the satellite distributions of intermediate-mass quiescent and star-forming centrals, $10.48<\log(M/M_\odot)<10.78$. Comparing to the Guo2011 semi-analytic model, the excess number of satellites indicates that quiescent centrals have halo masses 0.3 dex larger than star-forming centrals, even when the stellar-mass distributions are fixed. We use a simple toy model that relates halo mass and quenching, which roughly reproduces the observed quenched fractions and the differences in halo mass between star-forming and quenched galaxies only if galaxies have a quenching probability that increases with halo mass from $\sim$0 for $\log(M_h/M_\odot)\sim$11 to $\sim$1 for $\log(M_h/M_\odot)\sim$13.5. A single halo-mass quenching threshold is unable to reproduce the quiescent fraction and satellite distribution of centrals. Therefore, while halo quenching may be an important mechanism, it is unlikely to be the only factor driving quenching. It remains unclear why a high fraction of centrals remain star-forming even in relatively massive halos.Comment: 19 pages, 17 figures, accepted by ApJ. Information on ZFOURGE can be found at http://zfourge.tamu.ed

Resolved velocity profiles of galactic winds at Cosmic Noon

We study the kinematics of the interstellar medium (ISM) viewed "down the barrel" in 20 gravitationally lensed galaxies during Cosmic Noon ($z=1.5 - 3.5$). We use moderate-resolution spectra ($R\sim4000$) from Keck/ESI and Magellan/MagE to spectrally resolve the ISM absorption in these galaxies into $\sim$10 independent elements and use double Gaussian fits to quantify the velocity structure of the gas. We find that the bulk motion of gas in this galaxy sample is outflowing, with average velocity centroid \left=-141 km$\,$s$^{-1}$ ($\pm111$ km$\,$s$^{-1}$ scatter) measured with respect to the systemic redshift. 16 out of the 20 galaxies exhibit a clear positive skewness, with a blueshifted tail extending to $\sim -500$ km$\,$s$^{-1}$. We examine scaling relations in outflow velocities with galaxy stellar mass and star formation rate (SFR), finding correlations consistent with a momentum-driven wind scenario. Our measured outflow velocities are also comparable to those reported for FIRE-2 and TNG50 cosmological simulations at similar redshift and galaxy properties. We also consider implications for interpreting results from lower-resolution spectra. We demonstrate that while velocity centroids are accurately recovered, the skewness, velocity width, and probes of high velocity gas (e.g., $v_{95}$) are subject to large scatter and biases at lower resolution. We find that $R\gtrsim1700$ is required for accurate results for the gas kinematics of our sample. This work represents the largest available sample of well-resolved outflow velocity structure at $z>2$, and highlights the need for good spectral resolution to recover accurate properties.Comment: 42 pages, 37 figures (including appendix), Accepted for publication, Ap

Resolved Velocity Profiles of Galactic Winds at Cosmic Noon

We study the kinematics of the interstellar medium (ISM) viewed “down the barrel” in 20 gravitationally lensed galaxies during cosmic noon (z = 1.5–3.5). We use moderate-resolution spectra (R ∼ 4000) from Keck’s Echellette Spectrograph and Imager and Magellan/MagE to spectrally resolve the ISM absorption in these galaxies into ∼10 independent elements and use double Gaussian fits to quantify the velocity structure of the gas. We find that the bulk motion of gas in this galaxy sample is outflowing, with average velocity centroid v cent = − 141 km s−1 (±111 km s−1 scatter) measured with respect to the systemic redshift. A total of 16 out of the 20 galaxies exhibit a clear positive skewness, with a blueshifted tail extending to ∼ −500 km s−1. We examine scaling relations in outflow velocities with galaxy stellar mass and star formation rate, finding correlations consistent with a momentum-driven wind scenario. Our measured outflow velocities are also comparable to those reported for FIRE-2 and TNG50 cosmological simulations at similar redshift and galaxy properties. We also consider implications for interpreting results from lower-resolution spectra. We demonstrate that while velocity centroids are accurately recovered, the skewness, velocity width, and probes of high-velocity gas (e.g., v 95) are subject to large scatter and biases at lower resolution. We find that R ≳ 1700 is required for accurate results for the gas kinematics of our sample. This work represents the largest available sample of well-resolved outflow velocity structure at z &gt; 2 and highlights the need for good spectral resolution to recover accurate properties

Effect of local environment and stellar mass on galaxy quenching and morphology at 0.5<z<2.00.5<z<2.0

We study galactic star-formation activity as a function of environment and stellar mass over 0.5<z<2.0 using the FourStar Galaxy Evolution (ZFOURGE) survey. We estimate the galaxy environment using a Bayesian-motivated measure of the distance to the third nearest neighbor for galaxies to the stellar mass completeness of our survey, $\log(M/M_\odot)>9 (9.5)$ at z=1.3 (2.0). This method, when applied to a mock catalog with the photometric-redshift precision ($\sigma_z / (1+z) \lesssim 0.02$), recovers galaxies in low- and high-density environments accurately. We quantify the environmental quenching efficiency, and show that at z> 0.5 it depends on galaxy stellar mass, demonstrating that the effects of quenching related to (stellar) mass and environment are not separable. In high-density environments, the mass and environmental quenching efficiencies are comparable for massive galaxies ($\log (M/M_\odot)\gtrsim$ 10.5) at all redshifts. For lower mass galaxies ($\log (M/M)_\odot) \lesssim$ 10), the environmental quenching efficiency is very low at $z\gtrsim$ 1.5, but increases rapidly with decreasing redshift. Environmental quenching can account for nearly all quiescent lower mass galaxies ($\log(M/M_\odot) \sim$ 9-10), which appear primarily at $z\lesssim$ 1.0. The morphologies of lower mass quiescent galaxies are inconsistent with those expected of recently quenched star-forming galaxies. Some environmental process must transform the morphologies on similar timescales as the environmental quenching itself. The evolution of the environmental quenching favors models that combine gas starvation (as galaxies become satellites) with gas exhaustion through star-formation and outflows ("overconsumption"), and additional processes such as galaxy interactions, tidal stripping and disk fading to account for the morphological differences between the quiescent and star-forming galaxy populations.Comment: 29 pages, 15 figure, accepted for publication in Ap