A pig model of acute Staphylococcus aureus induced pyemia

<p>Abstract</p> <p>Background</p> <p>Sepsis caused by <it>Staphylococcus aureus </it>constitutes an important cause of morbidity and mortality in humans, and the incidence of this disease-entity is increasing. In this paper we describe the initial microbial dynamics and lesions in pigs experimentally infected with <it>S. aureus</it>, with the aim of mimicking human sepsis and pyemia.</p> <p>Methods</p> <p>The study was conducted in anaesthetized and intravenously inoculated pigs, and was based on bacteriological examination of blood and testing of blood for IL-6 and C-reactive protein. Following killing of the animals and necropsy bacteriological and histological examinations of different organs were performed 4, 5 or 6 h after inoculation.</p> <p>Results</p> <p>Clearance of bacteria from the blood was completed within the first 2 h in some of the pigs and the highest bacterial load was recorded in the lungs as compared to the spleen, liver and bones. This probably was a consequence of both the intravenous route of inoculation and the presence of pulmonary intravascular macrophages. Inoculation of bacteria induced formation of acute microabscesses in the lungs, spleen and liver, but not in the kidneys or bones. No generalized inflammatory response was recorded, i.e. IL-6 was not detected in the blood and C-reactive protein did not increase, probably because of the short time course of the study.</p> <p>Conclusion</p> <p>This study demonstrates the successful induction of acute pyemia (microabscesses), and forms a basis for future experiments that should include inoculation with strains of <it>S. aureus </it>isolated from man and an extension of the timeframe aiming at inducing sepsis, severe sepsis and septic shock.</p

Meteorological forcing, and corresponding hydrological model input and output used in the paper: The impact of hydrological model structure on the simulation of extreme runoff events.

The datasets used in Van Kempen et al., 2021 are included in this repository. These files consist of the original meteorological forcing, hydrological model input and hydrological model output. The README provides a description of the set-up of these datasets

The impact of hydrological model structure on the simulation of extreme runoff events

Hydrological extremes affect societies and ecosystems around the world in many ways, stressing the need to make reliable predictions using hydrological models. However, several different hydrological models can be selected to simulate extreme events. A difference in hydrological model structure results in a spread in the simulation of extreme runoff events. We investigated the impact of different model structures on the magnitude and timing of simulated extreme high- and low-flow events by combining two state-of-the-art approaches: a modular modelling framework (FUSE) and large ensemble meteorological simulations. This combination of methods created the opportunity to isolate the impact of specific hydrological process formulations at long return periods without relying on statistical models. We showed that the impact of hydrological model structure was larger for the simulation of low-flow compared to high-flow events and varied between the four evaluated climate zones. In cold and temperate climate zones, the magnitude and timing of extreme runoff events were significantly affected by different parameter sets and hydrological process formulations, such as evaporation. In the arid and tropical climate zones, the impact of hydrological model structures on extreme runoff events was smaller. This novel combination of approaches provided insights into the importance of specific hydrological process formulations in different climate zones, which can support adequate model selection for the simulation of extreme runoff events

Meteorological forcing, and corresponding hydrological model input and output used in the paper: The impact of hydrological model structure on the simulation of extreme runoff events.

The data sets used in Van Kempen et al., 2021 are included in this repository. These files consist of the original meteorological forcing, hydrological model input and hydrological model output.The readme file provides a description of the set-up of these data sets. </div

Meteorological forcing, and corresponding hydrological model input and output used in the paper: The impact of hydrological model structure on the simulation of extreme runoff events.

The datasets used in Van Kempen et al., 2021 are included in this repository. These files consist of the original meteorological forcing, hydrological model input and hydrological model output.The README provides a description of the set-up of these datasets. </div