The progesterone receptor PROGINS polymorphism is not related to oxidative stress factors in women with polycystic ovary syndrome

<p>Abstract</p> <p>Background</p> <p>Women with PCOS have been reported to be at increased risk of a number of gynaecological neoplasias, including endometrial, breast, and ovarian cancer. Studies of the possible association of genetic variation in progesterone receptor polymorphism with risk of ovarian and breast cancer have concentrated on a variant known as PROGINS.</p> <p>Methods</p> <p>Ninety-five young women with PCOS and 99 healthy control women were included in our study. All subjects underwent venous blood drawing for complete hormonal assays, lipid profile, glucose, insulin and <it>PROGINS </it>polymorphism genetic study.</p> <p>Results</p> <p>In PROGINS polymorphism results; in both control and the patient groups T1/T1 has been detected in high levels. But for genotype (p = 0.178) and allele (p = 0.555) frequencies both of the groups give similar results. Statistically significant difference has been detected on serum FSH levels for T1/T1 genotype according to T2/T2 genotype.</p> <p>Conclusion</p> <p>No relation has been detected between the inflammatory and oxidative stress factors, and PROGINS polymorphism alleles. This may be because the PCOS patients are young and their BMI means are normal and their CIMT and oxidative stress markers are like healthy women.</p

Effect Of G2706A and G1051A polymorphisms of the ABCA1 gene on the lipid, oxidative stress and homocystein levels in Turkish patients with polycystıc ovary syndrome

<p>Abstract</p> <p>Background</p> <p>Obesity, insulin resistance and hyperandrogenism, crucial parameters of Polycystic ovary syndrome (PCOS) play significant pathophysiological roles in lipidemic aberrations associated within the syndrome. Parts of the metabolic syndrome (low HDL and insulin resistance) appeared to facilitate the association between PCOS and coronary artery disease, independently of obesity. ABCA1 gene polymorphism may be altered this components in PCOS patients.</p> <p>In this study, we studied 98 PCOS patients and 93 healthy controls. All subjects underwent venous blood drawing for complete hormonal assays, lipid profile, glucose, insulin, malondialdehyde, nitric oxide, disulfide levels and ABCA genetic study.</p> <p>Results</p> <p>In PCOS group fasting glucose, DHEAS, 17-OHP, free testosterone, total-cholesterol, triglyceride, LDL-cholesterol and fibrinogen were significantly different compare to controls. The genotype ABCA G2706A distribution differed between the control group (GG 60.7%, GA 32.1%, AA 7.1%) and the PCOS patients (GG 8.7%, GA 8.7%, AA 76.8%). The frequency of the A allele (ABCAG2706A) was higher in PCOS patients than control group with 13,0% and 23,2%, respectively. In this study, the homocystein and insulin levels were significantly higher in PCOS patients with ABCA G1051A mutant genotype than those with heterozygote and wild genotypes.</p> <p>Conclusions</p> <p>We found higher percentage of AA genotype and A allele of ABCA G2706A in PCOS patients compare to controls. The fasting insulin and homocystein levels were significantly higher in PCOS patients with ABCA G1051A mutant genotype than those with heterozygote and wild genotypes.</p

Primer hiperlipidemi hastalarında ATP-bındıng cassette cholesterol transporter?1 G1051A ve G2706A gen polimorfizmi

Bu tezin, veri tabanı üzerinden yayınlanma izni bulunmamaktadır. Yayınlanma izni olmayan tezlerin basılı kopyalarına Üniversite kütüphaneniz aracılığıyla (TÜBESS üzerinden) erişebilirsiniz.Genetik faktörler metabolik olaylar üzerinde değişime yol açarak vücutta normalfonksiyonlarını yerine getirirler. Lipid metabolizması üzerinde etkili olan genlerinyapılarında meydana gelen aminoasid değişimleri (genetik polimorfizmler) geninmeydana getirdiği işlevleri de değiştirmektedir. Bu değişim sonucu lipoproteinmetabolizması ile ilgili proteinler, apolipoproteinler, reseptörler, enzimler veyakofaktörlerdeki değişiklikleri etkilenmektedir. Genetik değişikliklere bağlı ortaya çıkan budeğişimler lipid metabolizmasının primer bozuklukları olarak sınıflanır. Bu değişikliklerarasında ABCA1 geninde meydana gelen değişimler önemli rol oynayabilir. Biz buçalışmada primer hiperlipidemiye sahip bireylerde ABCA1 G2706A ve G1051A genpolimorfizmlerinin lipid düzeyleri, glikoz düzeyleri ve hs-CRP, fibrinojen gibi inflamatuarbelirteçler üzerine etkilerini araştırmayı amaçladık. Çalışmaya 60 erkek (ortalamayaşları 45.96±15.19) ve 78 kadın (ortalama yaşları 48.30±11.56) katıldı. Bu hastalarınperiferik lökosit hücrelerinden DNA izolasyonu yapıldı ve PCR ile ABCA1 G2706A veG1051A gen polimorfizmleri çalışıldı. Bu çalışmadan önce literatürde Türk toplumundaprimer hiperlipidemili hastalarda ABCA1 G1051A ve G2706A polimorfizminde allelsıklığını ve bunun koroner arter risk faktörleri ile ilişkisini gösteren bir veri yoktur. Buçalışmada metabolik sendrom ABCA1 gen G1051A polimorfizminde GG genotipine göre9GA genotipinde (p=0.001, OR: 5.816) ve AA genotipinde (p=0.039, OR: 3.619)metabolik sendrom sıklığı anlamlı yüksek saptandı.Metabolik sendrom varlığı ile ABCA1 G2706A polimorfizmi istatistiksel analizanlamlı bir ilişki saptanmadı [GG genotipine göre GA genotipinde (p=0.321, OR: 0.364),AA genotipinde (p=0.438, OR: 0.534)]. Çalışmaya katılan hastaların ABCA-1 2706 genpolimorfizmi analizinde AA genotipi taşıyanlar AG ve GG genotiplerine gore daha düşüksistolik kan basınçlarına sahip olduğu görüldü (p=0.027). ABCA1 G1051A genpolimorfizminde genotipler arasında sistolik ve diastolik kan basınçlarında herhangi birfark saptanmadı. ABCA1 G1051A (R219K) gen polimorfizmi ile HDL-kolesteroldüzeyleri arasında istatistiksel analiz yapıldığında HDL-kolesterol düzeyinin GGgenotipinde AA ve AG genotiplerine göre istatistiksel anlamlı yüksek olduğu saptandı(p=0.047

Diabetes Mellitus and Cancer

Diabetes and cancer are frequent diseases with important impact on human health all the world. Last epidemiologic studies suggests that patients with diabetes are at significantly higher risk for many forms of cancer. Type 2 diabetes and cancer share many risk factors. Increased insulin-like growth factor I (IGF-I) and reactive oxygen species can rol play in carcinogenesis. The systemic chronical inflammation which can result in a protumorigenic conditions. Hyperinsulinemia increases the risk of cancer in healthy people and it can partly explain obesity-cancer risk. The other point of view very important and difficult issue the medical treatment and dietary of diabetic patients with cancer

Diabetes Mellitus and Cancer

Abstract: Diabetes and cancer are frequent diseases with important impact on human health all the world. Last epidemiologic studies suggests that patients with diabetes are at significantly higher risk for many forms of cancer. Type 2 diabetes and cancer share many risk factors. Increased insulin-like growth factor I (IGF-I) and reactive oxygen species can rol play in carcinogenesis. The systemic chronical inflammation which can result in a protumorigenic conditions. Hyperinsulinemia increases the risk of cancer in healthy people and it can partly explain obesity-cancer risk. The other point of view very important and difficult issue the medical treatment and dietary of diabetic patients with cancer

4G/5G Polymorphism of PAI-1 gene and Alu-repeat I/D polymorphism of TPA gene in Turkish patients with polycystic ovary syndrome

WOS: 000249791700006PubMed ID: 17661167Purpose Polycystic ovary syndrome (PCOS) is one of the most encountered endocrine malfunctions. PCOS patients have enhanced activation of the blood coagulation system. Methods Eighty-six young women with PCOS and 70 healthy control women were included in our study. PCOS patients and controls were matched for age, body mass index, and allele frequency. Genetic analysis of TPAI and PAI-1 were performed in all subjects. Results and conclusions No statistically significant differences have been detected about the ratios of genotypes resulting from PAI-1 promotor 4G/5G gene polymorphism. PAI-1 765 4G/5G gene polymorphism and TPA gene's Alu-repeat insertion/deletion (I/D) polymorphism ratios were not different from the controls. In this study it is shown by the analysis of TPA gene's Alu-repeat insertion/deletion (I/D) polymorphism the PCOS patients with genotype II had lowers total cholesterol and LDL-cholesterol levels

The effect of 1 alpha,25(OH)(2)D-3 vitamin over oxidative stress and biochemical parameters in rats where Type 1 diabetes is formed by streptozotocin

WOS: 000271791300006PubMed ID: 18976933Introduction: The 1 alpha,25-dihydroxyvitamin D-3 [1 alpha,25(OH)(2)D-3] plays an essential role in mineral balance but has also been recognized as a powerful modulator of immune response. We aimed to examine the effect of the 1 alpha,25(OH)(2)D-3 treatment on insulin/c-peptide, catalase, superoxide dismutase (SOD), and blood glucose in rats that take streptozotocin (STZ). Methods: Forty pieces of male rats of Albino family whose average weights were 261.00 +/- 07.62 g were used in the study. Rats were made diabetic by giving STZ of 40 mg/kg during 5 days through intraperitoneal path. Some of the diabetic group and nondialbetic group were received 1 alpha,25(OH)(2)D-3. The levels of SOD, insulin, c-peptide, glucose, SOD, and catalase were measured at the zero, second, fourth, and sixth weeks. Results: Erythrocyte SOD levels didn't show a significant difference at the end of the sixth week in all groups when compared to the beginning. While erythrocyte catalase levels didn't show a significant difference in nondiabetic control and nondiabetic with vitamin D, and diabetic with vitamin D groups at the end of sixth week when compared to the beginning, a significant measurement was made in diabetic without vitamin D group. Maximal insulinitis scoring values were observed in diabetic without vitamin D that didn't receive 1 alpha,25(OH)(2)D-3 treatment. Conclusion: The highness of insulin and c-peptide levels in the group that received treatment when compared to other groups and the lowness of oxidative markers such as SOD, catalase in this study can be explained by the fact that 1 alpha,25(OH)(2)D-3 treatment prevents the intervention of apoptosis mechanism. Crown Copyright (C) 2009 Published by Elsevier Inc. All rights reserved