Chromoblastomycosis caused by rhinocladiella aquaspersa

A case of chromoblastomycosis of the hand caused by Rhinocladiella aquaspersa is described. The case was acquired locally in tropical Venezuela and was successfully treated with oral itraconazole

A comparison of isolation methods for black fungi degrading aromatic toxins

The prevalence of black fungi in the order Chaetothyriales has often been underestimated due to the difficulty of their isolation. In this study, three methods which are often used to isolate black fungi are compared. Enrichment on aromatic hydrocarbon appears effective in inhibiting growth of cosmopolitan microbial species and allows appearance of black fungi. We miniaturized the method for high-throughput purposes. The new procedure saves time, consumes less space and can process multiple samples simultaneously.info:eu-repo/semantics/publishedVersio

The origin of human pathogenicity and biological interactions in Chaetothyriales

Fungi in the order Chaetothyriales are renowned for their ability to cause human infections. Nevertheless, they are not regarded as primary pathogens, but rather as opportunists with a natural habitat in the environment. Extremotolerance is a major trend in the order, but quite diferent from black yeasts in Capnodiales which focus on endurance, an important additional parameter is advancing toxin management. In the ancestral ecology of rock colonization, the association with metabolite-producing lichens is signifcant. Ant-association, dealing with pheromones and repellents, is another mainstay in the order. The phylogenetically derived family, Herpotrichiellaceae, shows dual ecology in monoaromatic hydrocarbon assimilation and the ability to cause disease in humans and cold-blooded vertebrates. In this study, data on ecology, phylogeny, and genomics were collected and analyzed in order to support this hypothesis on the evolutionary route of the species of Chaetothyriales. Comparing the ribosomal tree with that of enzymes involved in toluene degradation, a signifcant expansion of cytochromes is observed and the toluene catabolism is found to be complete in some of the Herpotrichiellaceae. This might enhance human systemic infection. However, since most species have to be traumatically inoculated in order to cause disease, their invasive potential is categorized as opportunism. Only in chromoblastomycosis, true pathogenicity might be surmised. The criterion would be the possible escape of agents of vertebrate disease from the host, enabling dispersal of adapted genotypes to subsequent generations.info:eu-repo/semantics/publishedVersio

Potential Fungi Isolated From Anti-biodegradable Chinese Medicine Residue to Degrade Lignocellulose

Traditional Chinese medicine is one of the ancient medicines which is popular in Asian countries, among which the residue produced by the use of anti-biodegradables is endless, and causes significant adverse impacts on the environment. However, the high acidity of anti-biodegradable residues and some special biological activities make it difficult for microorganisms to survive, resulting in a very low degradation rate of lignocellulose in naturally stacked residues, which directly impedes the degradation of residues. We aimed to identify the fungal strains that efficiently biodegrade anti-biodegradable residue and see the possibility to improve the biodegradation of it and other agricultural wastes by co-cultivating these fungi. We isolated 302 fungal strains from anti-biodegradable residue to test hydrolysis ability. Finally, we found Coniochaeta sp., Fomitopsis sp., Nemania sp., Talaromyces sp., Phaeophlebiopsis sp. which inhabit the anti-biodegradable residues are capable of producing higher concentrations of extracellular enzymes. Synergistic fungal combinations (viz., Fomitopsis sp. + Phaeophlebiopsis sp.; Talaromyces sp. + Coniochaeta sp. + Fomitopsis sp.; Talaromyces sp. + Fomitopsis sp. + Piloderma sp. and Talaromyces sp. + Nemania sp. + Piloderma sp.) have better overall degradation effect on lignocellulose. Therefore, these fungi and their combinations have strong potential to be further developed for bioremediation and biological enzyme industrial production

The p38 MAPK/PMK-1 Pathway Is Required for Resistance to Nocardia farcinica Infection in Caenorhabditis elegance

Nocardia farcinica is an opportunistic pathogen that causes nocardiosis primarily in patients with compromised immune systems. In this study, we used the genetically tractable organism Caenorhabditis elegans as a model to study the innate immune responses to N. farcinica infection. We found that unlike other pathogenic bacteria such as Pseudomonas aeruginosa and Staphylococcus aureus, N. farcinica failed to kill adult worms. In another words, adult worms exposed to N. farcinica exhibited a normal lifespan, compared with those fed the standard laboratory food bacterium Escherichia coli OP50. Interestingly, deletion of three core genes (pmk-1, nsy-1 and sek-1) in the p38 MAPK/PMK-1 pathway reduced the survival of worm exposure to N. farcinica, highlighting a crucial role of this pathway for C. elegans in resistance to N. farcinica. Furthermore, our results revealed that N. farcinica exposure up-regulated the level of PMK-1 phosphorylation. The activation of PMK-1 promoted nuclear translocation of a transcription factor SKN-1/Nrf2, which in turn mediated N. farcinica infection resistance in C. elegans. Our results provide an excellent example that the integrity of immune system is key aspect for counteract with pathogenesis of N. farcinica

An experimental study on oxidizer treatment used to improve the seepage capacity of coal reservoirs

It is difficult to stimulate coal reservoirs in physical approaches, and the recovery factor of coalbed methane (CBM) can be enhanced by applying strong oxidizers through oxidation to stimulate coal reservoirs. At present, however, there have been very few studies on the oxidation of CBM and fewer experimental studies for systematically evaluating the effect of oxidation on the seepage capacity of coal reservoirs. In this paper, the coal samples taken from coal seams of the Jurassic Xishanyao Formation in the Heishan Coal Mine, Toksun, Xinjiang, were selected as the study objects. Hydrogen peroxide solution immersion experiments were carried out on columnar and powdered coal samples, respectively to measure the permeability of columnar coal samples, the dissolution rate of powdered coal samples and the property parameters of hydrogen peroxide solution. Then, the reaction mechanisms between coal samples and hydrogen peroxide and the mechanisms to improve the seepage capacity of coal reservoirs were analyzed by means of X-ray diffraction (XRD), scanning electron microscope (SEM), infrared spectrum and wetting angle measurement. Finally, the stimulation effect of oxidation was compared with that of acidification. The following research results were obtained. (1) The permeability of coal samples is increased significantly after oxidation to 1.4–3.2 times the original permeability. (2) A large number of micro-fractures and dissolved pores are formed in the coal samples after oxidation, and consequently pore connectivity is improved greatly. Thus, the amount of associative hydroxyl and carboxyl functional groups on the surface of the coal samples increases, and the water wettability on the surface reduces. (3) Organic matters and pyrites are oxidized and consumed easily, and the generated H+ and micro-molecular aliphatic acids further dissolve inorganic mineral components. (4) Oxidation also has the advantage of acidification for dissolving inorganic mineral components, so there is a low and controllable probability of generating coal powder. In conclusion, applying strong oxidizers has the potential to become a new technology for coal reservoir stimulation. Keywords: Coal rock, Oxidation, Permeability, Pore connectivity, Acidification, Micro-fracture and dissolved pore, Dissolving inorganic minerals, Coal reservoir stimulatio

Aging Characteristics of Rubber Modified Asphalts in Different Environmental Factors Combinations

Two kinds of rubber modified asphalts were investigated and compared with virgin asphalt. In order to get closer to engineering practice, different combinations of four environmental factors were merged into the laboratory aging simulation. Subsequently, conventional property tests, including softening point, viscosity, creep stiffness, creep rate, and fatigue cracking were conducted on asphalt samples. The performance difference of asphalt before and after aging was selected as an evaluation index for asphalt aging degree. The results indicate that two kinds of rubber modified asphalts show stronger resistant ability to temperature deformation and better resistance to traffic loading than virgin asphalt in all kinds of environmental factors combinations. Tests on chemical analyses were conducted to investigate the asphalt aging characteristics. The apparent morphology of rubber modified asphalts are described in detail under an environment scanning electron microscope (ESEM). The damage condition reflected in images reveals the aging degree caused by multiple environmental factors. Moreover, the thermogravimetric analysis (TG) confirms that three kinds of asphalts can maintain thermal stability in various environments. Additionally, new characteristic functional groups were not detected in the infrared (IR) spectra of rubber modified asphalts, which means they have stable antioxidant properties given that their oxidation degrees change slightly throughout the aging processes