Space-VLBI observations of OH maser OH34.26+0.15: low interstellar scattering

We report on the first space-VLBI observations of the OH34.26+0.15 maser in two main line OH transitions at 1665 and 1667 MHz. The observations involved the space radiotelescope on board the Japanese satellite HALCA and an array of ground radio telescopes. The map of the maser region and images of individual maser spots were produced with an angular resolution of 1 milliarcsec which is several times higher than the angular resolution available on the ground. The maser spots were only partly resolved and a lower limit to the brightness temperature 6x10^{12} K was obtained. The maser seems to be located in the direction of low interstellar scattering, an order of magnitude lower than the scattering of a nearby extragalactic source and pulsar.Comment: 8 pages, 2 tables, 9 figures, accepted for publication in MNRA

Anion-Sensitive Regions of L-Type CaV1.2 Calcium Channels Expressed in HEK293 Cells

L-type calcium currents (ICa) are influenced by changes in extracellular chloride, but sites of anion effects have not been identified. Our experiments showed that CaV1.2 currents expressed in HEK293 cells are strongly inhibited by replacing extracellular chloride with gluconate or perchlorate. Variance-mean analysis of ICa and cell-attached patch single channel recordings indicate that gluconate-induced inhibition is due to intracellular anion effects on Ca2+ channel open probability, not conductance. Inhibition of CaV1.2 currents produced by replacing chloride with gluconate was reduced from ∼75%–80% to ∼50% by omitting β subunits but unaffected by omitting α2δ subunits. Similarly, gluconate inhibition was reduced to ∼50% by deleting an α1 subunit N-terminal region of 15 residues critical for β subunit interactions regulating open probability. Omitting β subunits with this mutant α1 subunit did not further diminish inhibition. Gluconate inhibition was unchanged with expression of different β subunits. Truncating the C terminus at AA1665 reduced gluconate inhibition from ∼75%–80% to ∼50% whereas truncating it at AA1700 had no effect. Neutralizing arginines at AA1696 and 1697 by replacement with glutamines reduced gluconate inhibition to ∼60% indicating these residues are particularly important for anion effects. Expressing CaV1.2 channels that lacked both N and C termini reduced gluconate inhibition to ∼25% consistent with additive interactions between the two tail regions. Our results suggest that modest changes in intracellular anion concentration can produce significant effects on CaV1.2 currents mediated by changes in channel open probability involving β subunit interactions with the N terminus and a short C terminal region

RadioAstron gravitational redshift experiment: Status update

A test of a cornerstone of general relativity, the gravitational redshift effect, is currently being conducted with the RadioAstron spacecraft, which is on a highly eccentric orbit around Earth. Using ground radio telescopes to record the spacecraft signal, synchronized to its ultra-stable on-board H-maser, we can probe the varying flow of time on board with unprecedented accuracy. The observations performed so far, currently being analyzed, have already allowed us to measure the effect with a relative accuracy of 4 7 10−4. We expect to reach 2.5 7 10−5 with additional observations in 2016, an improvement of almost a magnitude over the 40-year old result of the GP-A mission