Photoresponse of Fullerene and AzafullerenePeapod Field Effect Transistors

9th IEEE Conference on Nanotechnology 200

Generation and Properties of Plasmas in Contact with Ionic Liquids

60th Gaseous Electronics Conferenc

Superposition of Parallel and Perpendicular Flow Velocity Shears in Magnetized Hybrid-Ion Plasmas

49th Annual Meeting of the Division of Plasma Physic

Continuous release of O2−/ONOO− in plasma-exposed HEPES-buffered saline promotes TRP channel-mediated uptake of a large cation

Although the externally controllable extracellular supply of the short-lived reactive oxygen and nitrogen species, such as O2•−, •NO, and ONOO−, could potentially manipulate cellular functions, their simple administration to cells is likely to be ineffective due to their rapid deactivation. In this study, we found a method of a continuous supply of O2•−/ONOO− over a few minutes, which is triggered by irradiation of a nonequilibrium atmospheric pressure plasma to commonly used organic buffers (e.g., 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid, HEPES). In addition, a continuous low-dose O2•−/ONOO− supply was shown to induce a physiologically relevant Ca2+ response and subsequently the uptake of a large cation mediated by transient receptor potential channel family member(s). Our results provide a novel approach to the continuous O2•−/ONOO− supply, requiring controllable and mass-volume treatments

Activation of plant immunity by exposure to dinitrogen pentoxide gas generated from air using plasma technology

Reactive nitrogen species (RNS) play an important role in plant immunity as signaling factors. We previously developed a plasma technology to partially convert air molecules into dinitrogen pentoxide (N(2)O(5)), an RNS whose physiological action is poorly understood. To reveal the function of N(2)O(5) gas in plant immunity, Arabidopsis thaliana was exposed to plasma-generated N(2)O(5) gas once (20 s) per day for 3 days, and inoculated with Botrytis cinerea, Pseudomonas syringae pv. tomato DC3000 (Pst), or cucumber mosaic virus strain yellow (CMV(Y)) at 24 h after the final N(2)O(5) gas exposure. Lesion size with B. cinerea infection was significantly (P < 0.05) reduced by exposure to N(2)O(5) gas. Propagation of CMV(Y) was suppressed in plants exposed to N(2)O(5) gas compared with plants exposed to the air control. However, proliferation of Pst in the N(2)O(5)-gas-exposed plants was almost the same as in the air control plants. These results suggested that N(2)O(5) gas exposure could control plant disease depending on the type of pathogen. Furthermore, changes in gene expression at 24 h after the final N(2)O(5) gas exposure were analyzed by RNA-Seq. Based on the gene ontology analysis, jasmonic acid and ethylene signaling pathways were activated by exposure of Arabidopsis plants to N(2)O(5) gas. A time course experiment with qRT-PCR revealed that the mRNA expression of the transcription factor genes, WRKY25, WRKY26, WRKY33, and genes for tryptophan metabolic enzymes, CYP71A12, CYP71A13, PEN2, and PAD3, was transiently induced by exposure to N(2)O(5) gas once for 20 s peaking at 1–3 h post-exposure. However, the expression of PDF1.2 was enhanced beginning from 6 h after exposure and its high expression was maintained until 24–48 h later. Thus, enhanced tryptophan metabolism leading to the synthesis of antimicrobial substances such as camalexin and antimicrobial peptides might have contributed to the N(2)O(5)-gas-induced disease resistance