A Soundscape of Sea Waves and Collaborative Action Research: The Case of the Nami-kozo Folktale of the Enshu-nada Sea, Japan

　This study examined rumbling emitted by the Enshu-nada Sea, with a focus on region-specific listening culture. Sea rumbling propagates over long distances in this area, where locals used its direction to predict the weather. Moreover, the connection between people and the sea is symbolized through a regionally transmitted folktale. We clarified the physical and sociocultural characteristics of sea rumbling through collaborative action research, thus demonstrating the significance of this approach in preserving and/or recovering local knowledge

13CO(J=1-0) On-the-fly Mapping of the Giant HII Region NGC 604: Variation in Molecular Gas Density and Temperature due to Sequential Star Formation

We present 13CO(J=1-0) line emission observations with the Nobeyama 45-m telescope toward the giant HII region NGC 604 in the spiral galaxy M 33. We detected 13CO(J=1-0) line emission in 3 major giant molecular clouds (GMCs) labeled as GMC-A, B, and C beginning at the north. We derived two line intensity ratios, 13CO(J=1-0)/12CO(J =1-0), R13/12, and 12CO(J=3-2)/12CO(J =1-0), R31, for each GMC at an angular resolution of 25" (100 pc). Averaged values of R13/12 and R31 are 0.06 and 0.31 within the whole GMC-A, 0.11 and 0.67 within the whole GMC-B, and 0.05 and 0.36 within the whole GMC-C, respectively. In addition, we obtained R13/12=0.09\pm0.02 and R31=0.76\pm0.06 at the 12CO(J=1-0) peak position of the GMC-B. Under the Large Velocity Gradient approximation, we determined gas density of 2.8 \times10^3 cm^-3 and kinetic temperature of 33+9-5 K at the 12CO(J=1-0) peak position of the GMC-B. Moreover, we determined 2.5 \times10^3 cm^-3 and 25\pm2 K as averaged values within the whole GMC-B. We concluded that dense molecular gas is formed everywhere in the GMC-B because derived gas density not only at the peak position of the GMC but also averaged over the whole GMC exceeds 10^3 cm^-3. On the other hand, kinetic temperature averaged over the whole GM-B, 25 K, is significantly lower than that at the peak position, 33 K. This is because HII regions are lopsided to the northern part of the GMC-B, thus OB stars can heat only the northern part, including the 12CO(J=1-0) peak position, of this GMC.Comment: 16 pages, 7 figures, PASJ in pres

New acoustic system for continuous measurement of river discharge and water temperature

AbstractIn many cases, river discharge is indirectly estimated from water level or streamflow velocity near the water surface. However, these methods have limited applicability. In this study, an innovative system, the fluvial acoustic tomography system (FATS), was used for continuous discharge measurement. Transducers with a central frequency of 30 kHz were installed diagonally across the river. The system's significant functions include accurate measurement of the travel time of the transmission signal using a GPS clock and the attainment of a high signal-to-noise ratio as a result of modulation of the signal by the 10th order M-sequence. In addition, FATS is small and lightweight, and its power consumption is low. Operating in unsteady streamflow, FATS successfully measured the cross-sectional average velocity. The agreement between FATS and acoustic Doppler current profilers (ADCPs) on water discharge was satisfactory. Moreover, the temporal variation of the cross-sectional average temperature deduced from the sound speed of FATS was similar to that measured by a temperature sensor near the bank

NRO M33 All-Disk Survey of Giant Molecular Clouds (NRO MAGiC): II. Dense Gas Formation within Giant Molecular Clouds in M33

We report the results of our observations of the 12CO (J=1-0) and 12CO (J=3-2) line emission of 74 major giant molecular clouds (GMCs) within the galactocentric distance of 5.1 kpc in the Local Group galaxy M33. The observations have been conducted as part of the Nobeyama Radio Observatory M33 All-disk survey of Giant Molecular Clouds project (NRO MAGiC). The spatial resolutions are 80 pc for 12CO (J=1-0) and 100 pc for 12CO (J=3-2). We detect 12CO (J=3-2) emission of 65 GMCs successfully. Furthermore, we find that the correlation between the surface density of the star formation rate, which is derived from a linear combination of Halpha and 24um emissions, and the 12CO (J=3-2) integrated intensity still holds at this scale. This result show that the star-forming activity is closely associated with warm and dense gases that are traced with the 12CO (J=3-2) line, even in the scale of GMCs. We also find that the GMCs with a high star-forming activity tend to show a high integrated intensity ratio (R3-2/1-0). Moreover, we also observe a mass-dependent trend of R3-2/1-0 for the GMCs with a low star-forming activity. From these results, we speculate that the R3-2/1-0 values of the GMCs with a low star-forming activity mainly depend on the dense gas fraction and not on the temperature, and therefore, the dense gas fraction increases with the mass of GMCs, at least in the GMCs with a low star-forming activity.Comment: 17 pages, 5 figures, Accepted for publication in PASJ, 2012, Vol. 64, No.

Comparative Secretome and Functional Analyses Reveal Glycoside Hydrolase Family 30 and Cysteine Peptidase as Virulence Determinants in the Pinewood Nematode Bursaphelenchus xylophilus

Pine wilt disease, caused by the pinewood nematode, Bursaphelenchus xylophilus, is one of the world’s most serious tree diseases. Although the B. xylophilus whole-genome sequence and comprehensive secretome profile have been determined over the past decade, it remains unclear what molecules are critical in pine wilt disease and govern B. xylophilus virulence in host pine trees. Here, a comparative secretome analysis among four isolates of B. xylophilus with distinct virulence levels was performed to identify virulence determinants. The four candidate virulence determinants of B. xylophilus highly secreted in virulent isolates included lipase (Bx-lip1), glycoside hydrolase family 30 (Bx-GH30), and two C1A family cysteine peptidases (Bx-CAT1 and Bx-CAT2). To validate the quantitative differences in the four potential virulence determinants among virulence groups at the protein level, we used real-time reverse-transcription polymerase chain reaction analysis to investigate these determinants at the transcript level at three time points: pre-inoculation, 3 days after inoculation (dai), and 7 dai into pine seedlings. The transcript levels of Bx-CAT1, Bx-CAT2, and Bx-GH30 were significantly higher in virulent isolates than in avirulent isolates at pre-inoculation and 3 dai. A subsequent leaf-disk assay based on transient overexpression in Nicotiana benthamiana revealed that the GH30 candidate virulent factor caused cell death in the plant. Furthermore, we demonstrated that Bx-CAT2 was involved in nutrient uptake for fungal feeding via soaking-mediated RNA interference. These findings indicate that the secreted proteins Bx-GH30 and Bx-CAT2 contribute to B. xylophilus virulence in host pine trees and may be involved in pine wilt disease