Development of High Efficiency Swing Compressor for R32 Refrigerant

In the age of global warming, energy saving features and overall reduction of environmental impact are critical components that must be addressed when developing new HVAC units. We chose the R32 refrigerant, with its lower LCCP as a more sustainable choice than the R410A. However, R32 has its drawbacks. Due to its smaller molecular weight, internal leakage loss is higher for the R32. Moreover, high discharge gas temperature decrease the reliability of the compressor, and make a large overheating loss increase. In this study, we will describe the technologies that were developed to overcome these drawbacks. We will also present the performance and reliability of the newly developed high efficiency swing compressor series for R32 refrigerants

ALSET - Japanese Air Launch System Ground Tests and Applications

The Air Launch System Enabling Technology (ALSET) project is a Japanese Ministry of Economy, Trade and Industry (METI) funded project whose purpose is to study air launch orbital payload delivery systems and related technologies. The project is a first step toward an operational commercial air launch system that will use a multistage solid rocket to deliver small payloads on the order of 100 to 200 kilograms into Low Earth Orbit (LEO). An air drop type launch approach to space transportation provides high reliability, flexibility, and responsiveness to meet the future needs of small satellite operators. ALSET culminates in a series of drop tests of an inert launch vehicle (a mass simulator) to demonstrate the technologies necessary for the operational system. The baseline system design uses a carriage extraction system method whereby the rocket is extracted from a C-130 aircraft on a TYPE-V platform. Two 28-foot extraction parachutes are used to pull the platform from the aircraft. Three 100-foot cargo parachutes are then deployed for deceleration prior to release of the rocket from the platform for launch. The baseline test site selected for the drop test is the Yuma Test Center (YTC) in Arizona, USA. The large drop zones available at the YTC are ideal for ALSET testing. Additionally, the YTC’s considerable experience with similar test activities, including the NASA Ares Jumbo Drop Test Vehicle drop tests, minimizes technical risks. The authors’ efforts to date completed the Critical Design Review (CDR) for the ALSET Drop Test in February and March 2015. The ALSET program is now ready for manufacturing for the ALSET Drop Test. As the prospective applications of ALSET to C4ISR missions, two case studies were conducted. The studies demonstrated the effectiveness of the air launch system. The responsiveness and flexibility of the air launch system enables small satellites to collect the ground information desired in short time, and to observe the target highfrequently by sequential satellite launches and forming a constellation

Cucumber mosaic virus infection in Arabidopsis: A conditional mutualistic symbiont?

A cucumber mosaic virus isolate, named Ho [CMV(Ho)], was isolated from a symptomless Arabidopsis halleri field sample containing low virus titers. An analysis of CMV(Ho) RNA molecules indicated that the virus isolate, besides the usual cucumovirus tripartite RNA genome, additionally contained defective RNA3 molecules and a satellite RNA. To study the underlying mechanism of the persistent CMV(Ho) infection in perennial A. halleri, infectious cDNA clones were generated for all its genetic elements. CMV, which consists of synthetic transcripts from the infectious tripartite RNA genomes, and designated CMV(Ho)tr, multiplied in A. halleri and annual Arabidopsis thaliana Col-0 to a similar level as the virulent strain CMV(Y), but did not induce any symptoms in them. The response of Col-0 to a series of reassortant CMVs between CMV(Ho)tr and CMV(Y) suggested that the establishment of an asymptomatic phenotype of CMV(Ho) infection was due to the 2b gene of CMV RNA2, but not due to the presence of the defective RNA3 and satellite RNA. The accumulation of CMV(Ho) 2b protein tagged with the FLAG epitope (2b.Ho-FLAG) in 2b.Ho-FLAG-transformed Col-0 did not induce any symptoms, suggesting a 2b-dependent persistency of CMV(Ho)tr infection in Arabidopsis. The 2b protein interacted with Argonaute 4, which is known to regulate the cytosine methylation levels of host genomic DNA. Whole genomic bisulfite sequencing analysis of CMV(Ho)tr- and mock-inoculated Col-0 revealed that cytosine hypomethylation in the promoter regions of 82 genes, including two genes encoding transcriptional regulators (DOF1.7 and CBP1), was induced in response to CMV(Ho)tr infection. Moreover, the increased levels of hypomethylation in the promoter region of both genes, during CMV(Ho)tr infection, were correlated with the up- or down-regulation of their expression. Taken altogether, the results indicate that during persistent CMV(Ho) infection in Arabidopsis, host gene expression may be epigenetically modulated resulting from a 2b-mediated cytosine hypomethylation of host genomic DNA

The whole blood transcriptional regulation landscape in 465 COVID-19 infected samples from Japan COVID-19 Task Force

「コロナ制圧タスクフォース」COVID-19患者由来の血液細胞における遺伝子発現の網羅的解析 --重症度に応じた遺伝子発現の変化には、ヒトゲノム配列の個人差が影響する--. 京都大学プレスリリース. 2022-08-23.Coronavirus disease 2019 (COVID-19) is a recently-emerged infectious disease that has caused millions of deaths, where comprehensive understanding of disease mechanisms is still unestablished. In particular, studies of gene expression dynamics and regulation landscape in COVID-19 infected individuals are limited. Here, we report on a thorough analysis of whole blood RNA-seq data from 465 genotyped samples from the Japan COVID-19 Task Force, including 359 severe and 106 non-severe COVID-19 cases. We discover 1169 putative causal expression quantitative trait loci (eQTLs) including 34 possible colocalizations with biobank fine-mapping results of hematopoietic traits in a Japanese population, 1549 putative causal splice QTLs (sQTLs; e.g. two independent sQTLs at TOR1AIP1), as well as biologically interpretable trans-eQTL examples (e.g., REST and STING1), all fine-mapped at single variant resolution. We perform differential gene expression analysis to elucidate 198 genes with increased expression in severe COVID-19 cases and enriched for innate immune-related functions. Finally, we evaluate the limited but non-zero effect of COVID-19 phenotype on eQTL discovery, and highlight the presence of COVID-19 severity-interaction eQTLs (ieQTLs; e.g., CLEC4C and MYBL2). Our study provides a comprehensive catalog of whole blood regulatory variants in Japanese, as well as a reference for transcriptional landscapes in response to COVID-19 infection

DOCK2 is involved in the host genetics and biology of severe COVID-19

「コロナ制圧タスクフォース」COVID-19疾患感受性遺伝子DOCK2の重症化機序を解明 --アジア最大のバイオレポジトリーでCOVID-19の治療標的を発見--. 京都大学プレスリリース. 2022-08-10.Identifying the host genetic factors underlying severe COVID-19 is an emerging challenge. Here we conducted a genome-wide association study (GWAS) involving 2, 393 cases of COVID-19 in a cohort of Japanese individuals collected during the initial waves of the pandemic, with 3, 289 unaffected controls. We identified a variant on chromosome 5 at 5q35 (rs60200309-A), close to the dedicator of cytokinesis 2 gene (DOCK2), which was associated with severe COVID-19 in patients less than 65 years of age. This risk allele was prevalent in East Asian individuals but rare in Europeans, highlighting the value of genome-wide association studies in non-European populations. RNA-sequencing analysis of 473 bulk peripheral blood samples identified decreased expression of DOCK2 associated with the risk allele in these younger patients. DOCK2 expression was suppressed in patients with severe cases of COVID-19. Single-cell RNA-sequencing analysis (n = 61 individuals) identified cell-type-specific downregulation of DOCK2 and a COVID-19-specific decreasing effect of the risk allele on DOCK2 expression in non-classical monocytes. Immunohistochemistry of lung specimens from patients with severe COVID-19 pneumonia showed suppressed DOCK2 expression. Moreover, inhibition of DOCK2 function with CPYPP increased the severity of pneumonia in a Syrian hamster model of SARS-CoV-2 infection, characterized by weight loss, lung oedema, enhanced viral loads, impaired macrophage recruitment and dysregulated type I interferon responses. We conclude that DOCK2 has an important role in the host immune response to SARS-CoV-2 infection and the development of severe COVID-19, and could be further explored as a potential biomarker and/or therapeutic target

Top down influence on visuo-tactile interaction modulates neural oscillatory responses

Multisensory integration involves bottom-up as well as top-down processes. We investigated the influences of top-down control on the neural responses to multisensory stimulation using EEG recording and time-frequency analyses. Participants were stimulated at the index or thumb of the left hand, using tactile vibrators mounted on a foam cube. Simultaneously they received a visual distractor from a light emitting diode adjacent to the active vibrator (spatially congruent trial) or adjacent to the inactive vibrator (spatially incongruent trial). The task was to respond to the elevation of the tactile stimulus (upper or lower), while ignoring the simultaneous visual distractor. To manipulate top-down control on this multisensory stimulation, the proportion of spatially congruent (vs. incongruent) trials was changed across blocks. Our results reveal that the behavioral cost of responding to incongruent than congruent trials (i.e., the crossmodal congruency effect) was modulated by the proportion of congruent trials. Most importantly, the EEG gamma band response and the gamma-theta coupling were also affected by this modulation of top-down control, whereas the late theta band response related to the congruency effect was not. These findings suggest that gamma band response is more than a marker of multisensory binding, being also sensitive to the correspondence between expected and actual multisensory stimulation. By contrast, theta band response was affected by congruency but appears to be largely immune to stimulation expectancy

ALSET - Japanese Air Launch System Concept and Test Plan

The Air Launch System Enabling Technology (ALSET) project is a Japanese Ministry of Economy, Trade and Industry (METI) funded project whose purpose is to study air launch orbital payload delivery systems and related technologies. The project is a first step toward an operational commercial air launch system that will use a multistage solid rocket to deliver small payloads on the order of 100 to 200 kilograms into Low Earth Orbit (LEO). An air drop type launch approach to space transportation provides high reliability, flexibility, and responsiveness to meet the future needs of small satellite operators. ALSET culminates in a series of drop tests of an inert launch vehicle (a mass simulator) to demonstrate the technologies necessary for the operational system. This paper will show the progress of system design and drop test planning. Trade studies are presented leading to a baseline system design and concept of operations. Factors considered in the trade studies include aircraft/launch vehicle interface, extraction and deceleration methods, and operational regulations. Commercial concept of operations to respond the demand to launch small satellites with responsiveness, advantages of the air launch system over existing launch system will be shown in this paper. The baseline system design uses a carriage extraction system method whereby the rocket is extracted from the aircraft on a carriage. A 15-foot pilot parachute is used to draw two 28-foot extraction parachutes and pull the carriage from the aircraft. Three G-11 cargo parachutes are then deployed for deceleration prior to release of the rocket from the carriage for launch. The baseline test site selected for the drop test is the Yuma Test Center (YTC) in Arizona, USA. The large drop zones available at the YTC are ideal for ALSET testing. Additionally, the YTC’s considerable experience with similar test activities, including the NASA Ares Jumbo Drop Test Vehicle drop tests, minimizes technical risks

Evolutional Launch Concept for Pico/Nano Satellite

As the capabilities (through standardization and modular design approaches) and users (from universities to research laboratories to private companies) of nanosatellites increase, there is a commensurate need for dedicated launch access to space. This paper reviews recent development efforts related to Nano-Launcher, an orbital payload launch service for nano and microsatellites (1-10 kg and 10-100 kg to orbit). The system uses mainly existing elements in combination, based upon existing solid stages (such as the SpaceSpike-1 and 2, stages evolved from the JAXA/ISAS S-520 solid rocket) along with existing air-launch aircraft (such as the F-104 and F-15). Nano-Launcher is deemed to have a lower development risk/cost and will be designed to be more responsive to nanosatellite customers than competing services. The program is being led by the authors with cooperation with Japan’s Ministry of Economy, Industry and Trade and Institute of Space (METI) and Space and Astronautical Science (ISAS) of JAXA. Key technologies currently being developed for the system include boost motor propulsion, non-pyrotechnic stage separation system, and lightweight and low-cost avionics. There is envisioned to be a breadth of Nano-Launcher payload delivery services available for suborbital and orbital customers utilizing different combinations of rocket stages and carrier aircraft