SAFETY AND EFFICACY STUDY OF HERBAL POLYPHYTO FORMULATIONS: FOR ITS LEARNING AND MEMORY ENHANCING PROPERTIES

Objective: The present study was designed to evaluate the effectiveness of the poly photo formulation for its learning and memory activity.Methods: The Indian origin test drug, FM7 phyto formulation compose of Convolulus pluricaulis, Habiscus rosasinnsis, Withania somnifera, Terminalia arjuna and Emblica officinalis, having the potential effect to improving memory studied at a dose of 50 mg and 100 mg/kg p. o. by using three different animal model like Elevated plus maze (EPM), Morris water maze (MWM) and Pole Climbing apparatus (PCA) for the effect of nootropic action; against standard drug Bacopa monnieri evaluated on the basis of transfer latency reduction on a rat, before and after drug administration.Results: On treatment with polyherbal formulation FM7 showed a significant effect on enhancing learning and memory properties. It was observed that significant (p<0.001) reduction in transfer latency in Elevated Plus Maze, Morris Water Maze test and escape latency in Pole Climbing Apparatus test as compared with the control; as well as standard Bacopa monnieri. Conclusion: The polyphyto formulation FM7 composed of (Convolulus pluricaulis 20%, Habiscus rosasinnsis 20%, Withania somnifera 20%, Terminalia arjuna 20% and Emblica officinalis 20%) found to be safe and effective in enhancing learning and memory properties.Keywords: Learning and memory, Convolulus pluricaulis, Phyllanthus Emblica, Withania somnifera, Habiscus rosasinnsis, Terminalia arjun

Original Article Nootropic Medicinal Plants; Evaluating Potent Formulation By Novelestic High throughput Pharmacological Screening (HTPS) Method: Evaluating Potent Formulation By Novelestic HTPS Method

The principle of this method was to screen the pharmacological activity of six prepared polyphyto formulations by using high throughput screening method for their nootropic action. The study was performed in three stages using one, two and three animals, respectively in a group. Test formulations were given p.o daily at the dose of 50 and 100 mg/kg body weight. The test formulations were compared with the standard drug Bacopa monnieri for learning and memory enhancement. The pharmacological response shown in single experimental animal was compared with that shown in two animals by using regression analysis. R2 value was determined by Least squares linear regression analysis to interpret as the proportion of the variance in the dependent variable that is predictable from the independent variable. Of the six formulations, three were considered for further evaluation in three groups (n=3). Finally, one formulation that showed maximum response was tested in a group of 12 animals. One way ANOVA was used for estimating the statistical significance. The best formulations FM-06 (compose of Celastrus paniculatus, Habiscus rosasinensis, Bacopa monnieri, Convolvulus pluricaulis, Phyllanthus emblica, and Mentha piperita) was selected by high throughput screening and results for twelve animals in a group were found to be statistically similar

Cell line toxicity study and pharmacological screening of effective nootropic herbal formulation in rat

The polyphyto drugs FM27; compose of Mentha piperita (10%), Ribes nigrum (20%), Cinnamomum zeylanicum (20%), Aloe vera (10%), Zingiber officinalis (5%), Glycine max (5%) and Avena sativa (30%) on memory acquisition and retention was studied using EPM, MWM and PCA test in rat using two different routes, one is per oral (FM27O) and another one is transcranial (FM27T). The test drug studied for in vitro safety profile screening by MTT assay in a different cell line. As per the result, cell line toxicity confirmed that formulation FM27 was non toxic in SH-SY-5Y human neuroblastoma cell line, Vero monkey normal kidney epithelial cells, and Chang liver cell line. Test drug FM27O studied at a dose of (PO 50 and 100 mg/kg), showed graded dose response and it was observed significantly (p <0.01) effect of memory improvement comparing standard group containing Bacopa monnieri (PO 50 and 100 mg/kg), whereas transcranially applied FM27T (10 mg/kg) showed a significant (p <0.001) effect of memory improvement comparing control group and observed similar effect like standard drug. Herbal formulations FM27 was found to be potent for treating dementia to improve memory. The cell line toxicity study report confirmed it was safe in higher dose also

Cell line toxicity study and pharmacological screening of effective nootropic herbal formulation in rat

23-30The polyphyto drugs FM27; compose of Mentha piperita (10%), Ribes nigrum (20%), Cinnamomum zeylanicum (20%), Aloe vera (10%), Zingiber officinalis (5%), Glycine max (5%) and Avena sativa (30%) on memory acquisition and retention was studied using EPM, MWM and PCA test in rat using two different routes, one is per oral (FM27O) and another one is transcranial (FM27T). The test drug studied for in vitro safety profile screening by MTT assay in a different cell line. As per the result, cell line toxicity confirmed that formulation FM27 was non toxic in SH-SY-5Y human neuroblastoma cell line, Vero monkey normal kidney epithelial cells, and Chang liver cell line. Test drug FM27O studied at a dose of (PO 50 and 100 mg/kg), showed graded dose response and it was observed significantly (p Bacopa monnieri (PO 50 and 100 mg/kg), whereas transcranially applied FM27T (10 mg/kg) showed a significant (p <0.001) effect of memory improvement comparing control group and observed similar effect like standard drug. Herbal formulations FM27 was found to be potent for treating dementia to improve memory. The cell line toxicity study report confirmed it was safe in higher dose also