Morphological characteristics and cell viability of coffee plants calli

The aim of this research was to characterize and compare two types of calli from leaf explants of Coffea arabica (cultivar Catiguá). Cells of different types of callus were successfully characterized regarding viability and internal and external morphological characteristics. It was obtained two morphologically distinct types of callus: (i) yellow friable and (ii) transparent watery. The yellow friable calli showed higher cell viability and embryogenic characteristics. Scanning and transmission electron microscopy showed embryogenic characteristics in cells of the yellow friable calli evidenced by the presence of small and isodiametric cells, while transparent watery calli showed elongated cells and large cytoplasm vacuolization

Genotypic variation of agronomic traits as well as concentrations of Fe, Zn, P and phytate in soybean cultivars

ABSTRACT The staple diet of most of the world population is provided by cereal, which present levels of iron (Fe) and zinc (Zn) below the nutritional demand of humans. Other factors that may substantially interfere for the low intake of Fe and Zn are the anti- nutritional factors present in the edible parts of plants, such as heavy metals and phytate. Hence, the objective of this study was to evaluate the genotypic variation in terms of yield (kg ha-1), plant height, insertion height of first pod, as well as concentrations of Fe, Zn, phosphorus (P) and the anti-nutrient phytate in grains of 24 soybean cultivars and to identify cultivars with potential for biofortification. The field experiment was conducted in Rio Verde, state of Goiás by the technological center of COMIGO. The experimental design used in the study was a randomized block design with 24 treatments and three replications. The results showed that there is genotypic variation for grain yield (kg ha-1), plant height, and insertion height of first pod, as well as for the concentrations of Zn, Fe, P and concentration of anti-nutient phytate in grains among the soybean cultivars. Non-significant or low correlations were found among the evaluated parameters except for the relationship between P and phytate (r=0.733). The diversity found among soybean cultivars offers genotypes such as 8197RR, M 7908RR and BRS 262 with potential to develop cultivars with better ability to accumulate nutrients in grains

Morphological characteristics and cell viability of coffee plants calli

The aim of this research was to characterize and compare two types of calli from leaf explants of Coffea arabica (cultivar Catiguá). Cells of different types of callus were successfully characterized regarding viability and internal and external morphological characteristics. It was obtained two morphologically distinct types of callus: (i) yellow friable and (ii) transparent watery. The yellow friable calli showed higher cell viability and embryogenic characteristics. Scanning and transmission electron microscopy showed embryogenic characteristics in cells of the yellow friable calli evidenced by the presence of small and isodiametric cells, while transparent watery calli showed elongated cells and large cytoplasm vacuolization