In vitro anti- biofilm and anti-bacterial activity of Sesbania grandiflora extract against Staphylococcus aureus

The main objective of this research is to investigate the anti-biofilm and anti-bacterial activity of Sesbania grandiflora (S. grandiflora) against Staphylococcus aureus. S. grandiflora extract were prepared and analyzed with UV –Vis spectroscopy, Fourier transform infrared spectroscopy, Dynamic light scattering. Biofilm forming pathogens were identified by congo-red assay. Quantification of Extracellular polymeric substance (EPS) particularly protein and carbohydrate were calculated. The efficacy of the herbal extract S. grandiflora and its inhibition against the pathogenic strain of S. aureus was also evaluated. The gradual decrease or disappearance of peaks reveals the reduction of protein and carbohydrate content in the EPS of S. aureus when treated with S. grandiflora. The antibacterial activity of S. grandiflora extract against the bacterial strain S. aureus showed that the extract were more active against the strain. To conclude, anti-biofilm and antibacterial efficacy of S. grandiflora plays a vital role over biofilm producing pathogens and act as a good source for controlling the microbial population

Biosynthesis of zinc oxide nanoparticles using culture filtrates of Aspergillus niger: Antimicrobial textiles and dye degradation studies

The main goal of undertaking the current investigation was to evaluate the extracellular synthesis of ZnO NPs using culture filtrates of Aspergillus niger. The obtained UV–Visible spectrum of the culture filtrate showed an absorption peak at 320 nm. The FTIR band showed the appearance of carboxylic acid and strong aromatic ring that could be responsible for the synthesis of ZnO NPs. Studies of the antimicrobial activity of ZnO NPs coated fabric showed a reduction of viable E. coli and S. aureus. 90% of decolorization of Bismarck brown dye was obtained by treating it with 100 µl ZnO NPs. The Germination (%) of the seeds of plants was lower with the raw dye when compared to the dye treated with ZnO NPs. The present study concludes that the A. niger mediated synthesis of ZnO NPs proved well in degrading dye and can be incorporated onto cotton fabrics since it reveals antibacterial activity. Keywords: A. niger, Zinc oxide nanoparticles, Bismarck brown, Pisum sativu