15 research outputs found
Synthesis by coprecipitation of india-stabilized zirconia and codoping with MoO3, WO3, TaO2.5, or NbO2.5 for application as thermal barrier coatings
Effect of Feedstock Powders on the Microstructural and Electrical Characteristics of 8 mol% Yttria-Stabilized Zirconia Plasma-Sprayed Coatings
Fine-Needle Aspiration Biopsy as a Preoperative Procedure in Patients with Malignancy in Solitary and Multiple Thyroid Nodules
An ubiquitin-dependent balance between mitofusin turnover and fatty acids desaturation regulates mitochondrial fusion
Production and Characterization of Ceramic Bushings from Alumina-Toughened Yttrium-Stabilized Tetragonal Zirconia Composites for Projection Welding Applications
Altered RNA processing and export lead to retention of mRNAs near transcription sites and nuclear pore complexes or within the nucleolus
Many protein factors are required for mRNA biogenesis and nuclear export, which are central to the eukaryotic gene expression program. It is unclear, however, whether all factors have been identified. Here we report on a screen of >1000 essential gene mutants in Saccharomyces cerevisiae for defects in mRNA processing and export, identifying 26 mutants with defects in this process. Single-molecule FISH data showed that the majority of these mutants accumulated mRNA within specific regions of the nucleus, which included 1) mRNAs within the nucleolus when nucleocytoplasmic transport, rRNA biogenesis, or RNA processing and surveillance was disrupted, 2) the buildup of mRNAs near transcription sites in 3′-end processing and chromosome segregation mutants, and 3) transcripts being enriched near nuclear pore complexes when components of the mRNA export machinery were mutated. These data show that alterations to various nuclear processes lead to the retention of mRNAs at discrete locations within the nucleus